We narrowed to 119,134 results for: mpi

1. pFastBacMam4_N_Flag_His8_eGFP_TEV

   Plasmid

   #178808

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Tags

   Flag, His8, eGFP, TEV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-ymTurquoise2-Kan

   Plasmid

   #224092

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with Kan selection

   Depositor

   Bas Teusink

   Insert

   ymTurquoise2
   

   Tags

   ymTurquoise2

   Expression

   Yeast

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAD12

   Plasmid

   #34832

   Depositor

   Cynthia Kenyon

   Article

   Dillin et al Science. 2002 Oct 25;298(5594):830-4.

   Insert

   None
   

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   June 11, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pAG36

   Plasmid

   #35126

   Depositor

   John McCusker

   Article

   Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

   Insert

   nat1
   

   Use

   Yeast replicating

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. IDG_TMC7_OE_1

   Plasmid

   #161674

   Purpose

   Overexpresses TMC7

   Depositor

   Mike McManus

   Insert

   TMC7 (TMC7 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EMSV-Myf5 (puro)

   Plasmid

   #14711

   Depositor

   Michael Rudnicki

   Article

   Sabourin et al Clin Genet. 2000 Jan . 57(1):16-25.

   Insert

   Myf5 (Myf5 Mouse)
   

   Expression

   Mammalian

   Available Since

   April 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOpen-BstpolLF

   Plasmid

   #165553

   Purpose

   Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   Bst DNA Polymerase, Large Fragment
   

   Use

   Synthetic Biology

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. CAG-FLEX-MCS-WPRE-SV40 - GCH2

   Plasmid

   #230815

   Purpose

   AAV cloning vector for CRE-dependent expression under the CAG promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR Ef1a-dCas9-BFP-KRAB

   Plasmid

   #217304

   Purpose

   lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

   Depositor

   Luke Gilbert

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Promoter

   Ef1alpha

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR]

    Plasmid

    #86449

    Purpose

    pBAc transposon with transformation marker for Tribolium transgenesis; Drives EGFP expression in the eyes; contains Gal4_ driven by Tribolium heatshock promoter; (Transformation plasmid)

    Depositor

    Gregor Bucher

    Insert

    3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR
    

    Expression

    Insect

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRL-TK CXCR4 4x

    Plasmid

    #11307

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    4 bulged bind sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Rr-luc

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-tMCK-mem-TurboID

    Plasmid

    #149410

    Purpose

    Muscle-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Insert

    mem-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Promoter

    tMCK

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX601-miniCMV-SaCas9-U6-LacZvsSaCas9 sgRNA

    Plasmid

    #107049

    Purpose

    All-in-one vectors expressing both SaCas9 and LacZ sgRNA

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-2T-Cdc42-wt

    Plasmid

    #12969

    Depositor

    Gary Bokoch

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

15. RABEP1A-c019

    Plasmid

    #175477

    Purpose

    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    RABEP1 (RABEP1 Human)
    

    Tags

    TEV-TwinStrep

    Expression

    Insect

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. BC-GPR56

    Plasmid

    #44197

    Depositor

    Lei Xu

    Insert

    human GPR56 (ADGRG1 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    LTR

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mScarletI-T2A-mTq2 pcDNA3.1

    Plasmid

    #230048

    Purpose

    Expresses mScarletI-T2A-mTq2 in human cells

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    mScarletI-T2A-mTq2
    

    Available Since

    Jan. 23, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pBS xenopus Islet1 (DM#219)

    Plasmid

    #15015

    Depositor

    Douglas Melton

    Article

    Kelly et al Dev Dyn. 2000 Aug . 218(4):615-27.

    Insert

    islet1
    

    Expression

    Bacterial

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid

    #221503

    Purpose

    CRISPRi machinery fish codon optimized expressing plasmid

    Depositor

    Alessandro Ori

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    Fish expression

    Mutation

    D10A and H839A point mutations

    Promoter

    Zebrafish Ubiquitin promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330 Mouse 3' Hp1a gRNA

    Plasmid

    #127898

    Purpose

    WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene

    Depositor

    Mark Groudine

    Insert

    gRNA/Cas9
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLIX403-EBFP2-ccdB-NatMx

    Plasmid

    #158559

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDDRFP-B1

    Plasmid

    #36290

    Depositor

    Robert Campbell

    Article

    Alford et al Chem Biol. 2012 Mar 23;19(3):353-60.

    Insert

    Dimerization dependent RFP-B1
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

23. AAV-CAG-FLEX-NES-jGCaMP8s-WPRE

    Plasmid

    #186046

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8s under the CAG promoter, Cre-dependent expression

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8s
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAS1-rab3P::his-24::mNeptune

    Plasmid

    #124350

    Purpose

    Nuclear Localized Pan-Neuronal mNeptune

    Depositor

    Andrew Leifer

    Insert

    rab-3, mNeptune (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. USH1CA1-TwinStrepII

    Plasmid

    #83202

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    USH1C (Ush1c Mouse)
    

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFastBacMam7_N_Flag_TEV

    Plasmid

    #178817

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Tags

    Flag, TEV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHN872

    Plasmid

    #178830

    Purpose

    Bacterial expression for human Saposin A

    Depositor

    Kazuhiro Abe

    Insert

    artificial gene encoding human Saposin A
    

    Tags

    GST, His8, TEV

    Expression

    Bacterial

    Mutation

    Codon-usage was optimized for bacterial expressio…

    Promoter

    T7

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTKIP-neo

    Plasmid

    #41064

    Depositor

    Edward Cox, Thomas Kuhlman

    Article

    Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAVS1-CAG-hCAS9-neo

    Plasmid

    #166026

    Purpose

    AAVS1 targeting vector carrying CAG-SpCas9-neo

    Depositor

    Kosuke Yusa

    Insert

    AAVS1-CAGCas9IRESneo
    

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME.E (pDHg16)

    Plasmid

    #24573

    Depositor

    David Pulleyblank

    Article

    Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

    Insert

    pME.E (pDHg16)
    

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

31. Flag-hTEX14

    Plasmid

    #190430

    Purpose

    Expresses Flag-tagged human TEX14 in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    TEX14 (TEX14 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ORFtag_LambdaN-SD_Frame3

    Plasmid

    #220986

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame3

    Depositor

    Alexander Stark

    Insert

    lambdaN
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ORFtag_LambdaN-SD_Frame1

    Plasmid

    #220984

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame1

    Depositor

    Alexander Stark

    Insert

    lambdaN
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ORFtag_LambdaN-SD_Frame2

    Plasmid

    #220985

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame2

    Depositor

    Alexander Stark

    Insert

    lambdaN
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV-CAG-FLEX-NES-jGCaMP8m-WPRE

    Plasmid

    #186045

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expression

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8m
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLIX403-EYFP-ccdB-Blast

    Plasmid

    #158551

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA5-FRT-TO-AID-EGFP

    Plasmid

    #80076

    Purpose

    Mammalian expression vector for C-terminal AID-EGFP tagging

    Depositor

    Andrew Holland

    Insert

    AID-EGFP
    

    Tags

    AID and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5-FRT-TO-EGFP-AID

    Plasmid

    #80075

    Purpose

    Mammalian expression vector for N-terminal EGFP-AID tagging

    Depositor

    Andrew Holland

    Insert

    EGFP-AID
    

    Tags

    AID and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSAR-MT-beta-gal

    Plasmid

    #16486

    Depositor

    Bert Vogelstein

    Article

    Morin et al Proc Natl Acad Sci U S A. 1996 Jul 23. 9

    Insert

    beta-galactosidase
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid

    #171939

    Purpose

    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein

    Depositor

    David Berson

    Insert

    APEX2-eGFPf
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUBC-Pfv-Sapphire

    Plasmid

    #203649

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. HOXB9-pMSCV-neo

    Plasmid

    #20998

    Depositor

    Corey Largman

    Insert

    HOXB9 (Hoxb9 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

43. pKSB- 3xP3-YFPsv40 GGGG AAGA

    Plasmid

    #174530

    Purpose

    3xP3-YFP fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    3xP3-YFP BsaI cassette
    

    Expression

    Insect

    Promoter

    3xP3

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAG Retro Puro Empty

    Plasmid

    #234458

    Purpose

    Retroviral vector for cloning and expressing your gene of interest. Puromycin selection.

    Depositor

    Melina Fan, Addgene Research Program

    Type

    Empty backbone

    Use

    Retroviral

    Available Since

    March 21, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pLNCX chick src K295R

    Plasmid

    #13659

    Depositor

    Joan Brugge

    Insert

    src K295R (SRC Chicken)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    K295R. Kinase dead mutant.

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

46. hTLR1 flag

    Plasmid

    #13081

    Depositor

    Ruslan Medzhitov

    Insert

    TLR1 (TLR1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-18 have been replaced with mammalia…

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

47. pRS40B

    Plasmid

    #35478

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast integrating vector

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pJJR81

    Plasmid

    #75029

    Purpose

    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Insert

    tagBFP2^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized tagBFP2

    Expression

    Worm

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pPro18

    Plasmid

    #17806

    Depositor

    Jay Keasling

    Article

    Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3-His-SUMO1

    Plasmid

    #159323

    Purpose

    Mammalian cell expression of mouse His-SUMO1

    Depositor

    Jianjun Zhao

    Insert

    His-SUMO1 (Sumo1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge