We narrowed to 17,075 results for: Por

1. pKrox24(2xD-E)dTomato

   Plasmid

   #200110

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element, no other promoter elemen…

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rlucrarr3-Flash5

   Plasmid

   #74133

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pMXs-IP- SCAT3.2

   Plasmid

   #172497

   Purpose

   SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.

   Depositor

   David Scadden

   Article

   Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

   Insert

   Amcyan
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   MLV LTR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-BLRR

   Plasmid

   #158958

   Purpose

   Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   BLRR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Rlucrarr3-Flash6

   Plasmid

   #74134

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pEMS2144

   Plasmid

   #104357

   Purpose

   AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

   Insert

   ssAAV-smCBA-EmGFP
   

   Use

   AAV

   Promoter

   smCBA

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Rlucrarr3-Flash1

   Plasmid

   #74129

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

8. Rlucrarr3-Flash4

   Plasmid

   #74132

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

9. Rlucrarr3-Flash3

   Plasmid

   #74131

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Rlucrarr3-Flash2

    Plasmid

    #74130

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Available Since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLentiPGK Puro DEST JNKKTRmCerulean3

    Plasmid

    #90232

    Purpose

    Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHMR1A

    Plasmid

    #102957

    Purpose

    gene cloning of MR1A

    Depositor

    Gennaro De Libero, Lucia Mori

    Article

    Lepore et al Nat Commun. 2014 May 15;5:3866. doi: 10.

    Insert

    MR1A cDNA (MR1 Human)
    

    Use

    Cloning

    Mutation

    Stop codon has been removed by HindIII site

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. cyt-GafD-mTurboID-V5

    Plasmid

    #184640

    Purpose

    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    cyt-GlycoID-V5
    

    Tags

    Nuclear export sequence and V5 tag

    Expression

    Mammalian

    Mutation

    GafD -> expressed aa22-178 ; BirA* -> expre…

    Promoter

    CMV

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. UAS-FRT-GFP-FRT-u(M)Cas9

    Plasmid

    #127388

    Purpose

    Cas9 inducible by removal of a GFP flip-out cassette

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    FRT-GFP-FRT-uORF(M)Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJET-CMV-GFP-P2A-K20-P2A-mCherry

    Plasmid

    #185618

    Purpose

    Ribosomal stalling reporter: positive control

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-K20(AAA)-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJET-CMV-GFP-P2A-K0-P2A-mCherry

    Plasmid

    #185619

    Purpose

    Ribosomal stalling reporter: empty

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-empty-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJET-CMV-GFP-P2A-I15-P2A-mCherry

    Plasmid

    #185620

    Purpose

    Ribosomal stalling reporter: 15x isoleucine

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-I15-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEvol-pAcFRS.1.t1

    Plasmid

    #73545

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.1.t1
    pAcFRS.1.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-Hygro-EF1A-LivePARBackbone

    Plasmid

    #176526

    Purpose

    Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21a-GFP-AURKA_K162M-mCherry

    Plasmid

    #99881

    Purpose

    Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Mutation

    K162M (kinase-dead)

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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