We narrowed to 6,530 results for: nls

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pCMV-160

Plasmid

#79840

Purpose

Expresses NLS-PpsR2-mVenus under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-mVenus

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRS513

Plasmid

#17283

Depositor

Nancy Craig

Article

Skelding et al EMBO J. 2002 Jul 1. 21(13):3494-504.

Insert

TnsB (tnsB E. coli transposon Tn7)

Tags

Chitin-binding domain-intein

Expression

Bacterial

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
L1_GATA23_2

Plasmid

#195912

Purpose

Level 1 PhiC31 cassette with LR dvp promoter: pGATA23-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pGATA23-PhiC31-NLS-tUBQ1

Expression

Plant

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P79

Plasmid

#195940

Purpose

Level 1 pGATA23 reporter : pGATA23-mScarlet-NLS-tRBCS

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pGATA23-mScarlet-NLS-tRBCS

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-267

Plasmid

#68770

Purpose

Sensor pair 1 V1

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-terminal Sensor 1 V1
N-Terminal Sensor 1 V1

Tags

NLS, flag, and nls

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-271

Plasmid

#68772

Purpose

Sensor pair 1 V3

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V3
N-Terminal Sensor 1 V3

Tags

NLS, flag, and nls

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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Sgf73_Sgf11/PCDFDuet

Plasmid

#26964

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

sgf11 (SGF11, SGF73 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pPS2192

Plasmid

#8923

Depositor

Pamela Silver

Article

Hood et al J Cell Sci 2001 Feb;114(Pt 3):589-97.

Insert

Clb2-deltaNLS-GFP (CLB2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Deletion of CLB2 bipartite NLS (aa 183-200), KpnI…

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only

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pMH186

Plasmid

#122837

Purpose

MS2-NLS (no stop codon) with GreenGate C-D flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

MS2-NLS, codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Tags

Nuclear localisation signal

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBC2101-reci

Plasmid

#117170

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 with SV40 NLS

Use

Lentiviral

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAP702e

Plasmid

#253945

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Article

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Begin-mNeonGreen:: SV40-NLS::Linker::Nucleoplasmin-NLS::mScarlet::Linker::T2A::Linker

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pAP701e

Plasmid

#253942

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Article

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Begin-mScarlet::SV40-NLS::Linker::Nucleoplasmin-NLS::mNeonGreen::Linker::T2A::Linker

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pGY251

Plasmid

#239813

Purpose

Express H2B-NLS-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

NLS of H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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His-MBP-FL-Efg1

Plasmid

#216395

Purpose

Expresses MBP-tagged full length Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-FL

Tags

6xHis tags-MBP

Expression

Bacterial

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-C-PrLD

Plasmid

#216398

Purpose

Expresses 6xHis-tagged C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-C-PrLD

Tags

6xHis-tags

Expression

Bacterial

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-DBD

Plasmid

#216399

Purpose

Expresses 6xHis-tagged DBD of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-DBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 2-181 and 357-554

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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L1_ARF19_2

Plasmid

#195914

Purpose

Level 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF19-PhiC31-NLS-tUBQ1

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P2

Plasmid

#195918

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3-PhiC31-NLS-tUBQ1

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P5

Plasmid

#195920

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3-PhiC31-NLS-DST-tUBQ1

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P17

Plasmid

#195922

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10-PhiC31-NLS-tUBQ1

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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