We narrowed to 31,527 results for: Eng;

1. pRA1EGFP

   Plasmid

   #193791

   Purpose

   Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PR promoter

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY2-MET-IV

   Plasmid

   #218117

   Purpose

   MET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY2-LYS-IV

   Plasmid

   #218118

   Purpose

   LYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETM6-T7-VHH72

   Plasmid

   #202487

   Purpose

   Expresses the nanobody VHH72 with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody VHH72
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNVLTv2-pvdIJD-Prha-base

   Plasmid

   #169244

   Purpose

   Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   rhaRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhaS

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPC009.106

   Plasmid

   #171142

   Purpose

   Expression of J106 scRNA on pBBR1-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J106 scRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. KO plasmid

   Plasmid

   #135970

   Purpose

   Expresses SpCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPMVAu8

   Plasmid

   #98297

   Purpose

   Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   PRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Actinin-sstFRET-GR

   Plasmid

   #83416

   Purpose

   FRET-based actinin tension sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

   Insert

   Actinin-sstFRET-GR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pBbA5c-MevT-MBI (JBEI-3085)

    Plasmid

    #87950

    Purpose

    the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    MevT-MBI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #83946

    Purpose

    Cas9-carrying vector with kanMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCK01_PalkS_alkS

    Plasmid

    #166502

    Purpose

    Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

    Depositor

    Sven Panke

    Article

    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

    Insert

    alkS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    L401G

    Promoter

    PalkS

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAB_PalkB_sfgfp_KanR

    Plasmid

    #166503

    Purpose

    Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

    Depositor

    Sven Panke

    Article

    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PalkB

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCas2C

    Plasmid

    #82374

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

    Plasmid

    #169586

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ARK2aa

    Plasmid

    #81013

    Purpose

    E.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Inserts

    MevTo-BBa1002-pTrc-Mkco-aphA
    and PMD
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    LacUV5 and Trc promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ptrc99a-pVhb-tdTER-fdh

    Plasmid

    #215649

    Purpose

    pBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    trans-enoyl-CoA reductase
    formate dehydrogenase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRC3EGFP

    Plasmid

    #193790

    Purpose

    Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PR promoter

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVH231-Tier1-PmPGK1-mTagBFP2

    Plasmid

    #169520

    Purpose

    Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric Tag blue fluorescent protein 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVH607-Tier1-PCREm4-Nluc

    Plasmid

    #169594

    Purpose

    Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVH233-Tier1-PmPGK1-eYFP

    Plasmid

    #169522

    Purpose

    Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTCW012

    Plasmid

    #83527

    Purpose

    Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET30b_T7Sav

    Plasmid

    #138588

    Purpose

    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    T7-tagged streptavidin variant with native C-terminus
    

    Use

    Synthetic Biology

    Tags

    T7-tag

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCI-T7Max-UTR1-deGFP-8xHis-T500

    Plasmid

    #178422

    Purpose

    Expression of deGFP via a T7 Promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

    Insert

    deGFP
    

    Use

    Synthetic Biology

    Tags

    8xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCORE-Kp53

    Plasmid

    #72241

    Purpose

    Contains the CORE cassette with KanMX4 and human p53 gene markers

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    kanMX4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBTRCk-pVhb-maACR

    Plasmid

    #215651

    Purpose

    pBBR1 Origin, KanR, with maACR gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    acyl-CoA reductase
    

    Use

    Tags

    Maltose binding protein and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACYC-pVhb-vfFadBA

    Plasmid

    #215650

    Purpose

    pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pY2

    Plasmid

    #218115

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pY3

    Plasmid

    #218116

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pY3-LYS-IV

    Plasmid

    #218119

    Purpose

    LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNVLTv2-pvdIJD-Ptrc-base

    Plasmid

    #169243

    Purpose

    Suicide vector for integrating expression cassette in P. putida (IPTG induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pENTR-Casp2

    Plasmid

    #50023

    Purpose

    pENTR vector for mouse Caspase-2

    Depositor

    Jamie Davies

    Article

    Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

    Insert

    Casp2 (Casp2 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pCORE-Hp53

    Plasmid

    #72242

    Purpose

    Contains the CORE cassette with hyg and human p53 gene markers

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    hyg
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCTCON2-GFP-TAG

    Plasmid

    #158131

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Introduced a stop codon in place of the tyrosine …

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCas5

    Plasmid

    #82378

    Purpose

    sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PJ23119

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFOX6-Tier1-PhCMV-TdTomato

    Plasmid

    #169515

    Purpose

    Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven dimeric red fluorescent protein TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pVH613

    Plasmid

    #169602

    Purpose

    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBbE1k-2

    Plasmid

    #59945

    Purpose

    dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase
    pterin-4a-carbinolamine dehydratase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pVH232-Tier1-PmPGK1-mRuby2

    Plasmid

    #169523

    Purpose

    Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge