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Showing: 481 - 520 of 947 results

  3. gRNA NIA TLS2

    Plasmid
    #196981
    Purpose
    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    AtNIA1 gRNAs fused to TLS2 tags
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pU6-26, pU6-29
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196981-plasmid-map-sequence-id-390454

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  4. gRNA NIA TLS1

    Plasmid
    #196980
    Purpose
    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    AtNIA1 gRNAs fused to TLS1 tags
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pU6-26, pU6-29
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196980-plasmid-map-sequence-id-390456

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  8. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Jeff Coleman
    Article
    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112065-plasmid-map-sequence-id-217190

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  12. pRH2502

    Plasmid
    #84379
    Purpose
    Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter
    Depositor
    Robert Husson
    Article
    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw
    Insert
    dcas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Aspartate at position 10 changed to Alanine, Hist…
    Promoter
    uv15tetO
    Available since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    84379-plasmid-map-sequence-id-173256

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  15. pMT1-24X-NOG(CmYLCV)

    Plasmid
    #202014
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue
    Inserts
    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    Use
    Tags
    GB1 and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    CmYLCV
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202014-plasmid-map-sequence-id-401203

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  17. pNHEJ-Cas9-recX

    Plasmid
    #158715
    Purpose
    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    cas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158715-plasmid-map-sequence-id-321939

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  22. pMT1-24X-NOG(AtUBI10)

    Plasmid
    #202016
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue
    Inserts
    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    Use
    Tags
    GB1 and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    AtUBI10
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202016-plasmid-map-sequence-id-401205

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  29. pCT

    Plasmid
    #60620
    Purpose
    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60620-plasmid-map-sequence-id-99574

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Showing: 481 - 520 of 947 results