We narrowed to 1,143 results for: nms

1. HA-Beclin 1

   Plasmid

   #86748

   Purpose

   N-terminal HA-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

   Insert

   human Beclin 1 (BECN1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ZIKV_Sensor_27B_LacZ

   Plasmid

   #75006

   Purpose

   Toehold switch sensor to detect Zika Virus

   Depositor

   Alexander Green, James Collins

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_sensor_27B_LacZ
   

   Use

   Tags

   LacZ

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2plus-mCerulean 156-239-GGGS

   Plasmid

   #162616

   Purpose

   Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   mCerulean
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   156-239

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2plus-mVenus1-155-GGGS

   Plasmid

   #162610

   Purpose

   Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   mVenus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains aa1-155 of mVenus

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MSCV-mir-17-92

   Plasmid

   #64100

   Purpose

   expresses mir-17-92

   Depositor

   Lin He

   Article

   Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

   Insert

   miR-17-92 (MIR17HG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p51.SRE.mCMV.CD63-GFP.wpre

   Plasmid

   #226773

   Purpose

   CD63-GFP in p52 (SRE backbone)

   Depositor

   Ferdinando Pucci

   Article

   Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

   Insert

   CD63 (CD63 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hSUMO1-HIS

   Plasmid

   #118361

   Purpose

   This plasmid expresses the full length human SUMO1.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hSUMO1 (SUMO1 Human)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Please see Depositor Comments

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hHSF1 S303/7A

   Plasmid

   #118365

   Purpose

   This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   S303A and S307A

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MiniCoopR mitfa:BRAF

   Plasmid

   #118846

   Purpose

   Expresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   BRAF V600E (BRAF Human, Zebrafish)
   

   Use

   Zebrafish expression, tol2

   Tags

   Expression

   Mutation

   BRAF V600E

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MiniCoopR mitfa:KIT K642E

    Plasmid

    #118849

    Purpose

    Expresses human KIT K642E mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    KIT K642E (KIT Human, Zebrafish)
    

    Use

    Zebrafish expression, tol5

    Tags

    Expression

    Mutation

    KIT K642E

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE

    Plasmid

    #122056

    Purpose

    Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P

    Depositor

    Stephen Tapscott

    Article

    Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

    Insert

    MD(ND2bHLH)WCS (Myod1, Neurod2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    MyoD chimeric protein substituted with the NeuroD…

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hSUMO1 GFP

    Plasmid

    #118360

    Purpose

    This plasmid expresses the full length human SUMO1.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hSUMO1 (SUMO1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MiniCoopR mitfa:NRAS

    Plasmid

    #118847

    Purpose

    Expresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    NRAS Q61R (NRAS Human, Zebrafish)
    

    Use

    Zebrafish expression, tol3

    Tags

    Expression

    Mutation

    NRAS Q61R

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHDR-CDKN2A-Ex2-CMV-EGFP

    Plasmid

    #110734

    Purpose

    Recombination template for replacing CDKN2A Exon2 with CMV-EGFP

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pECNUS4

    Plasmid

    #184887

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC009 LshCas13a locus with targeting spacer

    Plasmid

    #91900

    Purpose

    LshCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with targeting spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. FAK2_HUMAN_D0

    Plasmid

    #79747

    Purpose

    This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    FAK2 (PTK2B Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pC011 LwCas13a locus with targeting spacer

    Plasmid

    #91903

    Purpose

    LwCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with targeting spacer
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. MiniCoopR mitfa:KIT L576P

    Plasmid

    #118848

    Purpose

    Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    KIT L576P (KIT Human, Zebrafish)
    

    Use

    Zebrafish expression, tol4

    Tags

    Expression

    Mutation

    KIT L576P

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. STK24_HUMAN_D0

    Plasmid

    #79742

    Purpose

    This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK24 (STK24 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pCyCEN_Lisp2mCherry_hsp70_GFP

    Plasmid

    #137169

    Purpose

    Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

    Depositor

    Clemens Kocken

    Article

    Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

    Inserts

    mCherry
    Nanoluc
    dihydrofolate reductase
    Green Fluorescent Protein
    

    Use

    Unspecified

    Tags

    T2A

    Expression

    Mutation

    Promoter

    P. cynomolgi hsp70 and P. cynomolgi lisp2

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3.1-TUBG2

    Plasmid

    #171966

    Purpose

    Expression in cell lines of the TUBG2 gene

    Depositor

    Maria Alvarado-Kristensson

    Article

    Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

    Insert

    TUBG2 (TUBG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Removal of the internal TUBG2-EcoRI site by chang…

    Promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. myc-Atg14 (71-181)

    Plasmid

    #86753

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 71-181

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. myc-Atg14 (396-492)

    Plasmid

    #86755

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 396-492

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. myc-Atg14 (181-413)

    Plasmid

    #86754

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 181-413

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. myc-Atg14 (1-181)

    Plasmid

    #86752

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 1-181

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. myc-Atg14 (201-492)

    Plasmid

    #86751

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 201-492

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. myc-Atg14 (1-200)

    Plasmid

    #86750

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    Atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 1-200

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

    Plasmid

    #118845

    Purpose

    Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    ptena and ptenb (ptena, ptenb Zebrafish)
    

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2plus-mVenus1-155-I152L-GGGS

    Plasmid

    #162613

    Purpose

    Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-155-I152L

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. hHSF1 K298R

    Plasmid

    #118362

    Purpose

    This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K298R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ZIKV_Sensor_32B_LacZ

    Plasmid

    #75007

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    Alexander Green, James Collins

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_32B_LacZ
    

    Use

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pC012 LwCas13a locus with nontargeting spacer

    Plasmid

    #91904

    Purpose

    LwCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with non-targeting spacer
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. SLK_HUMAN_D0

    Plasmid

    #79733

    Purpose

    This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SLK (SLK Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

35. ZIKV_Trigger_27B

    Plasmid

    #75008

    Purpose

    Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

    Depositor

    Alexander Green, James Collins

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_27B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. myc-Atg14 S29D

    Plasmid

    #86757

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S29D

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAVA-LGF2(fs)-Gal11p(fs)

    Plasmid

    #127483

    Purpose

    Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    One nucleotide inserted between lambda CI and LGF…

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAVA-Gal11p-LGF2(fs)

    Plasmid

    #127482

    Purpose

    Negative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    Gal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LGF2 is frame shifted by the insert of one nucleo…

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. myc-Atg14 S29A

    Plasmid

    #86756

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ZIKV_Trigger_32B

    Plasmid

    #75009

    Purpose

    Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

    Depositor

    Alexander Green, James Collins

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_32B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge