We narrowed to 818 results for: VILL;

1. CRAF_S259A_pcDNA3.1

   Plasmid

   #86505

   Purpose

   Expresses CRAF_S259A in mammalian cells

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CRAF (RAF1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S259A

   Promoter

   CMV

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-6P-1 AUP1 292-410

   Plasmid

   #185331

   Purpose

   Bacterial expression of AUP1 with GST fusion protein for binding assays

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 292-410 only

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL-p21UTRm1&2

   Plasmid

   #20880

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Predicted miRNA binding site 1 (Position ~420) GC…

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

   Plasmid

   #107163

   Purpose

   Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme

   Depositor

   Jose Carlos Cameselle

   Article

   Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.

   Insert

   F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pECE-M2-BAIAP2 S366A

   Plasmid

   #31657

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   BAI1-associated protein 2 S366A (BAIAP2 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   changed Serine 366 to Alanine

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

6. CRAF_S29A_pcDNA3.1

   Plasmid

   #86506

   Purpose

   Expresses CRAF_S29A in mammalian cells [Edit]

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CRAF (RAF1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S29A

   Promoter

   CMV

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LRG/Foxa1 e2.4

   Plasmid

   #105511

   Purpose

   Lentiviral expression of Foxa1 DNA-binding domain (exon 2) targeting sgRNA

   Depositor

   Christopher Vakoc

   Article

   Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

   Insert

   Foxa1 (sgRNA, e2.4)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FUW-M2-PPP1R12C S452A

   Plasmid

   #31679

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   Protein Phosphatase 1 Regulatory Subunit 12C S452A (PPP1R12C Human)
   

   Use

   Lentiviral

   Tags

   M2

   Expression

   Mammalian

   Mutation

   changed Serine 452 to Alanine

   Promoter

   Available since

   April 12, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pGEX-BAIAP2 S366A

   Plasmid

   #31676

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   BAI1-associated protein 2 S366A (BAIAP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed Serine 366 to Alanine

   Promoter

   Available since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pSpCas9 (BB)-2A-GFP-RB1sg

    Plasmid

    #229857

    Purpose

    single guide RNA targeting human RB1 gene

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

    Insert

    RB1sgRNA (RB1 Human)
    

    Use

    Tags

    The gamma-tubulin sgRNA is coexpressed with 3xFLA…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-GG-hUbC-EBPF2-gRNA-FAM13A

    Plasmid

    #185550

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting FAM13A

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    FAM13A gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p2702-AGER-gRNA1

    Plasmid

    #216471

    Purpose

    to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Insert

    sgRNA targeting human AGER locus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p2703-AGER-gRNA2

    Plasmid

    #216472

    Purpose

    to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Insert

    sgRNA targeting human AGER locus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p2704-AGER-gRNA3

    Plasmid

    #216473

    Purpose

    to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Insert

    sgRNA targeting human AGER locus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p2705-AGER-eGFP

    Plasmid

    #216474

    Purpose

    donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Insert

    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

    Plasmid

    #216477

    Purpose

    lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-XPACK-DNAJC5 (L115R)- HaloTag

    Plasmid

    #205712

    Purpose

    Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    HaloTag and XPACK (GCINSKRKD)

    Expression

    Mammalian

    Mutation

    L115R

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-DEAD XPACK-DNAJC5 (L115R)

    Plasmid

    #205718

    Purpose

    Mammalian expression of DEAD XPACK-DNAJC5 (L115R)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Dead XPACK (GCINIKRKD)

    Expression

    Mammalian

    Mutation

    L115R

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCINeo-gp78 Mutant G2BR

    Plasmid

    #185355

    Purpose

    Assessing cellular requirement for G2BRgp78

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    aa 595-600 KRFLNK to AGAAGG

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-6P-1 AUP1 61-410

    Plasmid

    #185334

    Purpose

    Bacterial expression of AUP1 with GST fusion protein for binding assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 61-610 only

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA3-AUP1 A397D

    Plasmid

    #185342

    Purpose

    Assessing G2BR function in AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    Flag, 6xHis

    Expression

    Mammalian

    Mutation

    A397D

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-C1-G2BRAUP1

    Plasmid

    #185343

    Purpose

    Expression of soluble G2BR, amino acids 378-404 of human AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 aa 378-404 (G2BR) (AUP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 378-404 only

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Chr1q_Cassette-Proximal_gRNA

    Plasmid

    #195127

    Purpose

    gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr1q gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV-GG-hUbC-EBPF2-gRNA-DSP

    Plasmid

    #185549

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting DSP

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    DSP gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR-Mm_MyoGEF(WT)

    Plasmid

    #136327

    Purpose

    Mouse MyoGEF-WT in Gateway pENTR

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    PLEKHG6 (Plekhg6 Mouse)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV-PIG-N3FLAG-TAF12

    Plasmid

    #105593

    Purpose

    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    silent mutations to make it resistant to the targ…

    Promoter

    MSCV-LTR

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MSCV-PIG-N3FLAG-TAF12(50-130)

    Plasmid

    #105595

    Purpose

    retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    truncation fragments containing aa (50-130), and …

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. xCIAR_pcDNA5/FRT/TO

    Plasmid

    #86504

    Purpose

    Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    xCIAR (SOS1 Human)
    

    Use

    Frt

    Tags

    Expression

    Mammalian

    Mutation

    F929A and T968L in SOScat

    Promoter

    CMV (tet operator)

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDY1623 NOLC1 sgRNA 2

    Plasmid

    #234834

    Purpose

    sgRNA 2 for NOLC1 STITCHR insertion

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDY1622 NOLC1 sgRNA 1

    Plasmid

    #234833

    Purpose

    sgRNA 1 for NOLC1 STITCHR insertion

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

    Plasmid

    #207878

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAVS1-PDi-CRISPRn

    Plasmid

    #73500

    Purpose

    Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    Cas9
    rtTA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid

    #170977

    Purpose

    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    CShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    SspB and mCherry

    Expression

    Mutation

    Isoform X2, deleted aminoacids 1 - 1388

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pW17

    Plasmid

    #158207

    Purpose

    2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLX303-PCDH7-a

    Plasmid

    #108538

    Purpose

    Expresses human PCDH7 transcript variant a in mammalian cells

    Depositor

    Kathryn O’Donnell

    Article

    Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

    Insert

    PCDH7 transcript variant a (PCDH7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid

    #73499

    Purpose

    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDY0750 pCAG-NLS-BceINT

    Plasmid

    #179112

    Purpose

    BceINT expression vector

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLV-Hygro-EF1A-EGFP-PARP1

    Plasmid

    #176146

    Purpose

    EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUC119-gRNA

    Plasmid

    #52255

    Purpose

    Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    Arabidopsis U6 polymerase III promoter

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

    Plasmid

    #207880

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLV-Hygro-EF1A-BRCT2-Linker-eGFP

    Plasmid

    #176085

    Purpose

    EGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    BRCT2 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pLV-Hygro-EF1A-BRCT1-Linker-eGFP

    Plasmid

    #176074

    Purpose

    EGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    BRCT1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

    Plasmid

    #216478

    Purpose

    lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    YAP5SA
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    S61A, S109A, S127A, S164A, S381A

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCDNA3-AUP1-FLAG-DATDCUE

    Plasmid

    #185340

    Purpose

    Assessing domain requirement sof AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    deletion of aa 90-337 corresponding to the acyl t…

    Promoter

    Available since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDY0738 pCAG-NLS-SscINT

    Plasmid

    #179113

    Purpose

    SscINT expression vector

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-SscINT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pDY0739 pCAG-NLS-SacINT

    Plasmid

    #179114

    Purpose

    SacINT expression vector

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-SacINT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLVX-TetOne-Puro-PSMD4

    Plasmid

    #202666

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    proteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCDNA3-AUP1-FLAG-DCUE

    Plasmid

    #185339

    Purpose

    Assessing domain requirements of AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    deletion of aa 293-337 corresponding to the CUE d…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pDY0312 PhiBT Integrase

    Plasmid

    #179111

    Purpose

    PhiBT Integrase expression vector

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    PhiBT Integrase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge