We narrowed to 5,300 results for: pcdna3.1

1. pcDNA3.1-TFEBS211A-MYC (#805)

   Plasmid

   #99957

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   6xHIs and Myc

   Expression

   Mammalian

   Mutation

   S211A

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

   Plasmid

   #182287

   Purpose

   Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   

   Use

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

   Plasmid

   #117484

   Purpose

   Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   SpyCatcher002-6aa-sfCherry2(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-hAsCpf1(TYCV) (pY210)

   Plasmid

   #89351

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-HLA-Flag-natT1R3

   Plasmid

   #113950

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from HLA class I …

   Expression

   Mammalian

   Mutation

   residues 21-852

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1+-Tornado-split-nLuc

   Plasmid

   #212612

   Purpose

   For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-split-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   C373T mutation in CVB3

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/Myc-His(-)-HSulf-2

   Plasmid

   #13004

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 2 (SULF2 Human)
   

   Use

   Tags

   6x His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/hChR2(H134R)-mCherry

   Plasmid

   #20938

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. RGS-6xHis-BLIMP-1-pcDNA3.1-

    Plasmid

    #52518

    Purpose

    expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Blimp1 (PRDM1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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