4,134 results

1. hAAVS1 1R TALEN

   Plasmid

   #35432

   Purpose

   TALEN designed to target the human AAVS1 locus

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   hAAVS1 1R TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-Flag MKK6

   Plasmid

   #13517

   Depositor

   Roger Davis

   Article

   Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3 HA Arf6 ActQ67L

   Plasmid

   #10835

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 Q67L (ARF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q67L. Defective in GTP hydrolysis. Constitutive…

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HA-EglN1-pcDNA3

   Plasmid

   #18963

   Depositor

   William Kaelin

   Article

   Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

   Insert

   Egg laying Nine - 1 (EGLN1 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. Puro-Cas9 donor

   Plasmid

   #58409

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. hAAVS1 1L TALEN

   Plasmid

   #35431

   Purpose

   TALEN designed to target the human AAVS1 locus

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   hAAVS1 1L TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PH-Btk-GFP

   Plasmid

   #51463

   Purpose

   Is a biosensor for PIP3

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   BTK (aa 1–177) (BTK Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-HA2-DDB1

   Plasmid

   #19909

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   Damage-specific DNA binding protein 1 (DDB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   please see depositor comment below

   Promoter

   Available since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXs-Stat3-C

   Plasmid

   #13373

   Purpose

   Retroviral expression of dominant active mouse Stat3

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   signal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed Alanine 661 to cysteine and asparagine 66…

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA4TO-24xGCN4_v4-sfGFP

    Plasmid

    #61058

    Purpose

    A direct fusion of sfGFP to the 24xGCN4_v4 peptide array

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    24xGCN4_v4 peptide array and sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1436 pcDNA3 Flag HA

    Plasmid

    #10792

    Purpose

    pcDNA3 vector with added Flag, HA tags

    Depositor

    William Sellers

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

    Plasmid

    #31482

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce Target) EF Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry has M9S and M16L mutations. This is what …

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid

    #84563

    Purpose

    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    

    Use

    Lentiviral

    Tags

    myc tag (9x)

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-Cyclin D1

    Plasmid

    #19927

    Depositor

    Yue Xiong

    Article

    Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

    Insert

    Cyclin D1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP-Actin-7

    Plasmid

    #56421

    Purpose

    Localization: Actin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pmScarlet_alphaTubulin_C1

    Plasmid

    #85045

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Alpha-Tubulin (TUBA1B Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWPXL-Sox9

    Plasmid

    #36979

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Sox9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

    Plasmid

    #141386

    Purpose

    Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-M (M SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. MSCV-BCL6-IRES-GFP

    Plasmid

    #31391

    Depositor

    Hilda Ye

    Article

    Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

    Insert

    B-cell lymphoma 6 (BCL6 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge