9,002 results

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pAAV-mDlx-GFP-Fishell-1

Plasmid

#83900

Purpose

GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

eGFP

Use

AAV

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pmScarlet_C1

Plasmid

#85042

Purpose

Mammalian expression of mScarlet

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiPGK DEST H2B-iRFP670

Plasmid

#90237

Purpose

Lentiviral vector to express H2B-iRFP670 under PGK promoter

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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YFP-FKBP (YF)

Plasmid

#20175

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP (YF): (FKBP1A Human)

Expression

Mammalian

Mutation

FKBP was taken out from pC4M-F2E with a flanking …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pFGC-pcoCas9

Plasmid

#52256

Purpose

A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

Depositor

Jen Sheen

Insert

35SPPDK:pcoCas9:NOS cassette

Use

CRISPR; Plant expression

Promoter

constitutive 35SPPDK promoter

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cmyc

Plasmid

#16011

Purpose

mammalian expression of human cmyc

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

cmyc (MYC Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

Plasmid

#85428

Purpose

Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-12

Use

Lentiviral

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-EN479

Plasmid

#86234

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse rosa26 locus

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163056

Purpose

mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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Pyronic/pcDNA3.1(-)

Plasmid

#51308

Purpose

Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

Insert

Pyronic

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pBT374

Plasmid

#125615

Purpose

Expresses evoFERNY-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max-NG

Expression

Mammalian

Mutation

H102P D104N

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-L1

Plasmid

#52637

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Left AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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dimericTomato-2xrGBD

Plasmid

#176098

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Lentiviral

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pD40-His/V5-c-Myc

Plasmid

#45597

Depositor

Rosalie Sears

Article

Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

Insert

c-Myc (Myc Mouse)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pBS598 EF1alpha-EGFPcre

Plasmid

#11923

Purpose

Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

EGFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pET264-pUC 24xMS2V6 Loxp KANr Loxp

Plasmid

#104393

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pFPV-mCherry

Plasmid

#20956

Depositor

Olivia Steele-Mortimer

Article

Drecktrah et al Traffic. 2008 Dec . 9(12):2117-29.

Insert

mCherry

Expression

Bacterial

Available Since

June 3, 2009

Availability

Academic Institutions and Nonprofits only

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