We narrowed to 8,446 results for: gnal

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HA-MBP-TEVpCS-ADGRG4 CTFdeltaTA-FLAG

Plasmid

#217735

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG4 (ADGRG4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2727

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG2 CTFdeltaTA-FLAG

Plasmid

#217733

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG2 (ADGRG2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-612

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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CMV BARK D110A p2A mCherry

Plasmid

#117690

Purpose

CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Tags

HA / FLAG

Expression

Mammalian

Mutation

D110A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PTEN

Plasmid

#116866

Purpose

Recruitable PTEN dual-specificity phosphatase

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PTEN (PTEN Human)

Tags

FKBP12 and mCherry

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHL 1158

Plasmid

#53007

Purpose

pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
DsrA sRNA
ompC
sodB

Use

Synthetic Biology

Tags

gfp and mCherry

Expression

Bacterial

Mutation

contains -56 to +141 relative to start codon and …

Promoter

pCon, pLlacO-1, and pLtetO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pPBbsr2-Raichu-454HRasCT

Plasmid

#176029

Purpose

A FRET biosensor for HRas activity

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

Raichu-Ras-HRasCT

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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AP-1 mu1-GFP

Plasmid

#173432

Purpose

Expression of AP-1 Mu1 subunit with a terminal GFP

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP1M1 (Ap1m1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4

Plasmid

#14615

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(2)-CRY2-mCherry

Plasmid

#160441

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CKAR2

Plasmid

#123348

Purpose

Improved FRET-based biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

CKAR2

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pScalps eSpCas9_Zsgreen gRNA scaffold

Plasmid

#188449

Purpose

Expression of eSpCas9 tagged with Zsgreen

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF1-alpha

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERalpha (ESR1 Human)

Expression

Yeast

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
CMV10-3xFlag Skp2 WT

Plasmid

#81115

Purpose

mammalian expression of Skp2 WT

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-GIT1

Plasmid

#15226

Depositor

Rick Horwitz

Article

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

L425V, E476K and L583P

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-pcDNA3-PKCgamma-cys1Acys1B

Plasmid

#21190

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKCgamma-cys1Acys1B (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

Available Since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab5a-c-Myc

Plasmid

#79801

Purpose

Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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bArr1-V5

Plasmid

#201492

Purpose

Expression of the beta-arrestin1 containing a C-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr1-V5 (ARRB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP682-Smad2

Plasmid

#118943

Purpose

Mammalian expression of human Smad2 protein

Depositor

Won Do Heo

Article

Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

Insert

iRFP682-Smad2 (SMAD2 Human)

Tags

iRFP682

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TetON-Bmpr1A-ca

Plasmid

#217536

Purpose

AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

constitutively active BMP receptor 1a (BMPR1A Human)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

Tet operon (TetO)

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pRN3P-NLS-EGFP-deadBtgh-3NLS

Plasmid

#194470

Purpose

Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

Depositor

Mathieu Boulard

Article

Formichetti et al Dev Cell 10 Jan 2024

Insert

BtGH84

Use

For in vitro transcription from t3 promoter after…

Tags

SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

Mutation

Aspartic Acid 242 (in Dennis et al.) changed to A…

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-Eevee-ROCK-NES

Plasmid

#209918

Purpose

A FRET biosensor for ROCK activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

Eevee-ROCK-NES

Use

Lentiviral

Tags

ECFP and YPet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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VPS26C_pcDNA6.2/EmGFP-Bsd

Plasmid

#176982

Purpose

Mammalian expression vector encoding VPS26C and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

VPS26C (VPS26C Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35516

Purpose

AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

Arch3.0-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1

Plasmid

#19838

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-HBXIP

Plasmid

#42326

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

HBXIP (LAMTOR5 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hGfap-R-CaMP1.07-WPRE-SV40

Plasmid

#164140

Purpose

AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

Depositor

Hirokazu Hirai, Hajime Hirase

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

R-CaMP1.07

Use

AAV

Promoter

hGFAP

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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12x PRE TK luc

Plasmid

#206163

Purpose

Luciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

12X PRE TK luciferase

Use

Luciferase

Expression

Mammalian

Promoter

min TK

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K118-119R

Plasmid

#63564

Purpose

Expression of human H2A with K118R and K119R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 118 and Lysine 119 are now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pRK5 TGF beta type I receptor (T202D) Flag

Plasmid

#14833

Depositor

Rik Derynck

Article

Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Tags

flag

Expression

Mammalian

Mutation

Threonine 202 mutated to aspartate. Constitutivel…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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