We narrowed to 16,553 results for: por

1. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174647

   Purpose

   Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   CMV (with TetOn)

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS1210

   Plasmid

   #29107

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple238 (TRH Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcBac1-EcTyr-TAG

   Plasmid

   #228782

   Purpose

   EcTyr aaRS/tRNA system for mammalian ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   BstR
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.23-WIPI1promotor

   Plasmid

   #206840

   Purpose

   The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

   Depositor

   Tassula Proikas-Cezanne

   Article

   Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

   Insert

   WIPI1 promotor (partial)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-Teton-puro-shFDPS #2

   Plasmid

   #198760

   Purpose

   conditional knockdown of FDPS

   Depositor

   Hyonchol Jang

   Article

   Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

   Insert

   shFDPS #2 (FDPS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-Teton-puro-shFDPS #1

   Plasmid

   #198759

   Purpose

   conditional knockdown of FDPS

   Depositor

   Hyonchol Jang

   Article

   Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

   Insert

   shFDPS #1 (FDPS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174646

   Purpose

   Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

   Promoter

   CMV (with TetOn)

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500

   Plasmid

   #92315

   Purpose

   Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   B1 domain of protein L (PL) mutant K16TAG
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70b (p70a promoter with one mutation)

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS1396

   Plasmid

   #29194

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple146 (NTSR1 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEMS1174

    Plasmid

    #29088

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple240 (UGT8 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

    Plasmid

    #89991

    Purpose

    Donor template for generation of SOX2-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

    Insert

    SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-pHluorin_M153R-CD63-mScarlet

    Plasmid

    #172118

    Purpose

    Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet and pHluorin_M153R in a small extracellu…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-pHluorin_M153R-CD63

    Plasmid

    #172117

    Purpose

    Fluorescent reporter for CD63-positive exosome secretion

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    pHluorin_M153R in a small extracelluar loop of CD…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-Beatrix

    Plasmid

    #167528

    Purpose

    Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

    Depositor

    Gian Michele Ratto

    Article

    Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

    Insert

    Beatrix
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGolt-mCherry / pGolt3-mCherry

    Plasmid

    #73297

    Purpose

    Visualization of Dendritic Golgi Satellite

    Depositor

    Michael Kreutz

    Article

    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

    Inserts

    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    

    Use

    Tags

    ER export and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MLRV

    Plasmid

    #118069

    Purpose

    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Inserts

    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Proinsulin-NanoLuc in pLX304

    Plasmid

    #62057

    Purpose

    Luminescent reporter of insulin secretion

    Depositor

    David Altshuler

    Article

    Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

    Insert

    Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Inserted NanoLuc into C-peptide of mouse Ins2 cod…

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMS1408

    Plasmid

    #29205

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple201 (SLC6A5 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-shMcu-1

    Plasmid

    #181868

    Purpose

    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-shMcu-2

    Plasmid

    #181867

    Purpose

    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge