We narrowed to 78,412 results for: rest
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Plasmid#211517PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterSV40 and minProAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only
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Lenti:FlipGFP-T2A-mCherry
Plasmid#223198PurposeLentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)DepositorInsertFlipGFP-T2A-mCherry
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
mycBioID2-13X Linker-MCS
Plasmid#92308PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsHumanized A. aeolicus BioID2 with R40G and MycExpressionMammalianPromoterCMVAvailable SinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-BioID2-MCS
Plasmid#74223PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsMyc and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minCMV
Plasmid#211518PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterSV40 and minCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PH1(WT)
Plasmid#86599PurposeExpresses eGFP-tagged PH(WT) domain of human Akt1DepositorInsertAkt1 PH domain (AKT1 Human)
ExpressionMammalianAvailable SinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVL-5
Plasmid#104583PurposeContains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functionsDepositorAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
SMT205
Plasmid#134817PurposeSMT201 with mutated RBS for DHFR, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 WT
Plasmid#78260PurposepsiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertfully complimentary let-7 target site
UseLuciferase; Microrna activityExpressionMammalianAvailable SinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
RNA motif plasmid cloning backbone
Plasmid#107253PurposeRNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kbDepositorInsertRNA motif plasmid cloning backbone
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1-F-tractin-mCherry
Plasmid#155218PurposeMonitor global F-actin distributionDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
TagsmCherryExpressionMammalianAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sst44-GFP
Plasmid#172310PurposeSST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneuronsDepositorInsertSst44
UseAAVPromoterpB-GlobinAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt1(WT)
Plasmid#86637PurposeExpresses eGFP-tagged human Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
ExpressionMammalianAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGAselect
Plasmid#195714PurposepGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceFeb. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLyGo-Kp-1
Plasmid#163143PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastPromoterAOX1Available SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastPromoternoAvailable SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR
Plasmid#126679PurposeFluorescent reporter for cyclin E/A-CDK and CDK4/6 activitiesDepositorInsertRb (a.a 886-928)
UseLentiviralTagsmCherryExpressionMammalianAvailable SinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
hJAM-A pcDNA3.1
Plasmid#70073PurposeExpresses human junctional adhesion molecule-A in mammalian cellsDepositorAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 MT
Plasmid#78261PurposepsiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated let-7 target site
UseLuciferase; Microrna activityExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CTCC to GA…Available SinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only