We narrowed to 7,236 results for: ALP

1. LXSN-Cx43-S282D

   Plasmid

   #60711

   Purpose

   retroviral expression of rat Cx43 S282D mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S282D

   Promoter

   LTR

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. 1068 pGL3 FasL promoter (no SV40 prom)

   Plasmid

   #9029

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   Fas ligand promoter (FASLG Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Removed the SV40 promoter from pGL3

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pHH0103_EEF1A1_GTP-EFTU-D2

   Plasmid

   #109811

   Purpose

   Protein expression and purification of EEF1A1_GTP-EFTU-D2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   EEF1A1_GTP-EFTU-D2 (EEF1A1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

   Plasmid

   #170217

   Purpose

   Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CASP8 (CASP8 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   C360A

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKTop::tse5-CT (1169-1281)

   Plasmid

   #192955

   Purpose

   For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT (1169-1281)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1281)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Ef1a-DIO-Egr3-EYFP-WPRE

   Plasmid

   #186417

   Purpose

   Overexpression of Egr3 transcript variant 1 CDS under EF-1α promoter

   Depositor

   Mary Kay Lobo

   Article

   Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

   Insert

   Early growth response 3 (Egr3 Mouse)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   EF-1-alpha

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pE-cadherin-GFP-N-WASP deltaVCA

   Plasmid

   #101283

   Purpose

   Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   delta VCA domain

   Promoter

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

   Plasmid

   #120563

   Purpose

   Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmCherry-3xNLS-OptoJNKi

   Plasmid

   #89740

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGB 35s:dCas:EDLL:tNos (GB1190)

    Plasmid

    #75404

    Purpose

    Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional Activator

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    dCas:EDLL
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40

    Plasmid

    #186967

    Purpose

    Lentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    DNMT3L (DNMT3L Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    rTetR fusion

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FKBP-DCRD-YFP

    Plasmid

    #87450

    Purpose

    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    DCRD (distal C-terminal regulatory domain) (CACNA1F Human)
    

    Use

    Tags

    6xHis-T7, FKBP, and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKCAG-mCherry-OptoJNKi

    Plasmid

    #89738

    Purpose

    Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmCherry-NES-OptoJNKi

    Plasmid

    #89739

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)-RIα(D/D+linker)-EGFP

    Plasmid

    #181842

    Purpose

    EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains only residues 12-112 of PKA RIα.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKCAG-H2BmCherry-OptoJNKi

    Plasmid

    #89741

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Optogenetics

    Tags

    Histone2B and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBullet-cg-c

    Plasmid

    #53070

    Purpose

    destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRIM24 gRNA (BRDN0001147238)

    Plasmid

    #77889

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM24

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PKN1 gRNA (BRDN0001162227)

    Plasmid

    #76276

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN1 (PKN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CFP-PCRD-FRB

    Plasmid

    #87449

    Purpose

    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
    

    Use

    Tags

    6xHis-T7, CFP, and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-FLEXfrt-SaCas9-U6-sgGria3

    Plasmid

    #124869

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. His-GINA3

    Plasmid

    #74980

    Purpose

    Expresses His tagged GINA3 protein in bacteria.

    Depositor

    Pradipta Ghosh

    Article

    Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

    Insert

    Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-45b-RUNX2(+10A)-IDR-mCherry

    Plasmid

    #145287

    Purpose

    Bacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2(+10A)-IDR (RUNX2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGB 35s:dCas:VP64:tNos (GB1189)

    Plasmid

    #75403

    Purpose

    Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional Activator

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    dCas:VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CD8a-cNLS-EGFP

    Plasmid

    #86052

    Purpose

    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    CD8a (CD8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CD8a luminal and transmembrane along with two SV4…

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PRKAA1 gRNA (BRDN0001147273)

    Plasmid

    #76254

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBullet-cg-n

    Plasmid

    #53069

    Purpose

    destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (N-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HNF4A_P2-285/-192

    Plasmid

    #31053

    Depositor

    Gerhart Ryffel

    Article

    Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -285 to -1 of P2 HNF4A promoter with mutation -19…

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TRIM24 gRNA (BRDN0001148301)

    Plasmid

    #77888

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM24

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HNF4A_P2-285/-169

    Plasmid

    #31052

    Depositor

    Gerhart Ryffel

    Article

    Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -285 to -1 of P2 HNF4A promoter with mutation -16…

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HNF4A_P2-285/-136

    Plasmid

    #31051

    Depositor

    Gerhart Ryffel

    Article

    Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -285 to -1 of P2 HNF4A promoter with mutation -13…

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LXSN-Cx43-Myc

    Plasmid

    #60694

    Purpose

    retroviral expression of Myc-tagged rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

33. LXSN-Cx43-YA/VD-Myc

    Plasmid

    #60702

    Purpose

    retroviral expression of Myc tagged rat Cx43 YA/VD mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    YA/VD (Y286A/V289D)

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

34. PRKCD gRNA (BRDN0001145526)

    Plasmid

    #76002

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCD

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCD (PRKCD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PRKCD gRNA (BRDN0001147700)

    Plasmid

    #76003

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCD

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCD (PRKCD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PRKCD gRNA (BRDN0001147061)

    Plasmid

    #76004

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCD

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCD (PRKCD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PIP4K2A gRNA (BRDN0001147809)

    Plasmid

    #76574

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP4K2A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP4K2A (PIP4K2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PIP4K2A gRNA (BRDN0001149528)

    Plasmid

    #76575

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP4K2A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP4K2A (PIP4K2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PIP4K2A gRNA (BRDN0001148000)

    Plasmid

    #76576

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP4K2A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP4K2A (PIP4K2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA-Cx43-YA/VD-Myc

    Plasmid

    #60701

    Purpose

    mammalian expression of Myc tagged rat Cx43 YA/VD mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    YA/VD (Y286A/V289D)

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

41. PRKAA1 gRNA (BRDN0001149089)

    Plasmid

    #76255

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pYI2165

    Plasmid

    #228347

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
    

    Use

    Affinity Reagent/ Antibody

    Tags

    AAA linker followed by FLAG-His6 and MF_ secretio…

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pYI2216

    Plasmid

    #228349

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    

    Use

    Affinity Reagent/ Antibody

    Tags

    AAA linker followed by FLAG-His6 and MF_ secretio…

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. TFORF0457

    Plasmid

    #142629

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pmCherry-AHPH

    Plasmid

    #187278

    Purpose

    Express mCherry-AHPH (RhoA biosensor)

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin and Pleckstrin homology domain (ANLN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    AHPH

    Promoter

    CMV

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pTag4C_ADRA1A-V2R-NTEV-tcs-GV

    Plasmid

    #227101

    Purpose

    ADRA1A receptor for split TEV assay

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    ADRA1A-NTEV (ADRA1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid

    #227321

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pPPI4-SNAP-CTLA-4-His6

    Plasmid

    #223584

    Purpose

    Protein purification of human CTLA4 with SNAP and 6xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Tags

    His6 and SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pGAL4-DBD-CEBPa_WT(N)

    Plasmid

    #215635

    Purpose

    For overexpression of Gal4-DBD CEBPa WT(N)

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    CEBPa(N) (CEBPA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pX330_FIGLA_cterm4

    Plasmid

    #222909

    Purpose

    Cas9/sgRNA plasmid for targeting FIGLA

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, FIGLA sgRNA 4 (FIGLA Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge