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pEE058

Plasmid

#176815

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

609

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE059

Plasmid

#176816

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

610

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE035

Plasmid

#176792

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

404

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Clim Actin MCH Puro (CAMP)

Plasmid

#104446

Purpose

Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.

Depositor

Elena Casacuberta, Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

mCherry

Use

Expression in corallochytrium (single-celled euka…

Promoter

actin

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-URH49 MUT

Plasmid

#110126

Purpose

Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

URH49 (DDX39A Human)

Tags

Flag

Expression

Insect

Mutation

4 amino acid mutations (RSFS motif changed to KSL…

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E MUT

Plasmid

#110122

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

4 mutations (KKLN motif changed to RSFS)

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Oct4deltaPE-53BP1mCherry

Plasmid

#141122

Purpose

Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells

Depositor

John Schimenti

Article

Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.

Insert

Fragment of human 53BP1 (TP53BP1 Human)

Use

Mouse Targeting

Tags

mCherry

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)

Plasmid

#112802

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 14_22 and 447_454 deleted

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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p3R112C/T180CHFm

Plasmid

#85091

Purpose

Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C in PCNA3, A74G in heme-FMN domain of …

Promoter

T7 promoter

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS1207

Plasmid

#29104

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple235

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

Plasmid

#113862

Purpose

Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc

Use

Lentiviral

Expression

Mammalian

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Expression

Mammalian

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Article

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Bmal1

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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CMV-LUC2CP/intron/ARE

Plasmid

#62858

Purpose

splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

Depositor

Gideon Dreyfuss

Article

Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

Insert

Luciferase

Use

Luciferase

Expression

Mammalian

Mutation

insertion of chimeric intron at nueotide poclsiti…

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pUltraI-Tet3.0[TAA]

Plasmid

#164580

Purpose

Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Inserts

Mb Tet3.0RS (R2-3)
Mm tRNA[TAA]

Expression

Bacterial

Mutation

L170G, N311G, C313A

Promoter

ProK and TacI

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHCD1C

Plasmid

#102954

Purpose

gene cloning of human CD1C

Depositor

Gennaro De Libero, Lucia Mori

Article

Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

Insert

CD1C cDNA (CD1C Human)

Use

Cloning

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAcBac1-EcTrp-TGA

Plasmid

#228780

Purpose

aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTrpRS
EcWtR

Use

Baculoviral

Expression

Mammalian

Mutation

TGA suppressor

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAcBac1-EcTyr-TAG

Plasmid

#228782

Purpose

EcTyr aaRS/tRNA system for mammalian ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTyrRS
EcYtR
BstR

Use

Baculoviral

Expression

Mammalian

Mutation

TAG suppressor

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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fluoPEER-GFP>BFP

Plasmid

#185477

Purpose

Fluorescent reporter for a Y66H mutation to convert a GFP to BFP.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

GFP-Y66-editing-site

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.23-5xE-box

Plasmid

#206841

Purpose

The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

Depositor

Tassula Proikas-Cezanne

Article

Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

Insert

5x E-box ((GCCACGTGCA)

Use

Luciferase

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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