We narrowed to 7,507 results for: PAC
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Plasmid#19146DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
UseTagsMycExpressionMammalianMutationhuman BRG1 aa 597-1407 (PCR product)PromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt
Plasmid#157702Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMMB67EH-dddAI-FLAG
Plasmid#186560PurposeBroad host range regulated expression of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)DepositorInsertdddAI
UseTagsFLAGExpressionBacterialMutationPromotertacAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-VPR-WT CjCas9 V-WT
Plasmid#169914PurposeExpression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.DepositorInsertCjCas9
UseCRISPRTags2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-FKBP-INPP5ED477N
Plasmid#202738Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-mHP1alpha
Plasmid#181911PurposeBacterial protein expression vector for HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAD54_CAD29_H104A_pvp008
Plasmid#124876PurposeExpresses hCAD His103Ala mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationmutated His103 to Ala, deleted amino acids 1-3 an…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt
Plasmid#157701Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)
Plasmid#197892PurposeCan be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInserthM4D/2A/GFP
UseAAVTagsExpressionMutationN/APromoterEF1aAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMYC BRGI aa1400-1648
Plasmid#19145DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
UseTagsMycExpressionMammalianMutationhuman BRG1 aa 1400-1648 (PCR product)PromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC
Plasmid#140203PurposeFungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.DepositorInsertcrRNA array elcA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPgpdAAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp8-HF_GC3opt
Plasmid#157699Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertNsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPSV39-dddAI-FLAG
Plasmid#186558PurposeBroad host range regulated expression of the DddA with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)DepositorInsertdddAI
UseTagsFLAGExpressionBacterialMutationPromoterlacUV5Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB(C460S)
Plasmid#183677PurposeRecombinant Salmonella Typhimurium SopB (catalytically inactive)DepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
UseTagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialMutationC460SPromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAD108_Arg273His_pCMV6Entry-hIRG1
Plasmid#124878PurposeExpresses hCAD Arg273His mutant in mammalian cellsDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsmyc and FLAG tagsExpressionMammalianMutationmutated Arg273 to HisPromoterCMVAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
G30, Cdc20-mCherry-AID tagging
Plasmid#130270PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombinationDepositorInsertCdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt
Plasmid#157693Purposemammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAD96_hIRG1_4-461_pvp008
Plasmid#124874PurposeExpresses hCAD Asn152Ser mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationmutated Asn152 to Ser, deleted amino acids 1-3 an…PromoterT7Available sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-APLP1ICD-IRES-hrGFP
Plasmid#107546PurposeAAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cellsDepositorInsertAPLP1ICD (APLP1 Human)
UseAAVTagsExpressionMutationNonePromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC131: pAAV.CMV-CasRx-VEGFA sgRNA array
Plasmid#203444PurposePlasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAsDepositorInsertsU6-VEGFA sgRNA array
RfxCas13d
UseAAVTagsHA and NLSExpressionMammalianMutationPromoterCMV and U6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM156: pAAV.CMV-Cas13e (GenScript CO)-VEGFA sgRNA
Plasmid#203452PurposePlasmid expressing active and codon optimised Cas13e with VEGFA gRNADepositorInsertsU6-VEGFA sgRNA
Cas13e
UseAAVTagsHA and NLSExpressionMammalianMutationPromoterCMV and U6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD139A
Plasmid#218129PurposeHybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of luxABDepositorInsertsUseTagsscP22ExpressionMutationW384A, Y386APromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine
Plasmid#202064PurposeInducible AAV activatorDepositorInsertAAV-Rapamycin Inducible-ZNF473KRAB-Citrine
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT312 AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator
Plasmid#202061PurposeInducible AAV activatorDepositorInsertAAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator
UseAAV; Aav with reporterTagsExpressionMammalianMutationITRs intactPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SopB(172-554)
Plasmid#183664PurposeN-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expressionDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium)
UseTagsEGFPExpressionMammalianMutationAmino acids 172-554PromoterCMVAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SopB(211-554)
Plasmid#183665PurposeN-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expressionDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium)
UseTagsEGFPExpressionMammalianMutationAmino acids 211-554PromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-2xMS2-lacO
Plasmid#181909PurposeSingle guide RNA with 2XMS2 loops targeting bacterial lac operatorDepositorInsertsgRNA-2XMS2-lacO
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-2xMS2-mSAT
Plasmid#181908PurposeSingle guide RNA with 2XMS2 loops targeting mouse major satellite repeatsDepositorInsertsgRNA-2XMS2-mSAT
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-10R
Plasmid#178664PurposeBacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues removed (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-10R (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationten Arg residues removedPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+8D
Plasmid#178674PurposeBacterial expression of N-terminally 6His tagged A1-LCD with eight Asp residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+8D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationeight Asp residues addedPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7R
Plasmid#178667PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+7R (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationseven Arg residues addedPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-2K
Plasmid#178668PurposeBacterial expression of N-terminally 6His tagged A1-LCD with two Lys residues removed (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-2K (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwo Lys residues removedPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+4D
Plasmid#178673PurposeBacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+4D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationfour Asp residues addedPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-6R
Plasmid#178665PurposeBacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues removed (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-6R (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationsix Arg residues removedPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+2R
Plasmid#178666PurposeBacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+2R (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwo Arg residues addedPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-6R+6K
Plasmid#178670PurposeBacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-6R+6K (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationsix Arg residues replaced with LysPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+12D
Plasmid#178675PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twelve Asp residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+12D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwelve Asp residues addedPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-3R+3K
Plasmid#178669PurposeBacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-3R+3K (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationthree Arg residues replaced with LysPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7F-7Y
Plasmid#178660PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD +7F-7Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationseven Tyr residues replaced with PhePromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+12E
Plasmid#178676PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twelve Glu residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+12E (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwelve Glu residues addedPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only