Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 15,549 results for: pET

Clear keywords
Showing: 6601 - 6650 of 15549 results

  2. pVRC8400-SARS-CoV-2-S2P-B.1.526-S477N-AVI

    Plasmid
    #176329
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.526 variant containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.429 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T95I, D253G, S477N, D614G, A701V
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176329-plasmid-map-sequence-id-347138

    Enlarge

  3. pVRC8400-SARS-CoV-2-S2P-B.1.526-E484K-AVI

    Plasmid
    #176330
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.526 variant containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.429 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T95I, D253G, E484K, D614G, A701V
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176330-plasmid-map-sequence-id-347139

    Enlarge

  4. pVRC8400-SARS-CoV-2-S2P-B.1.617.1-AVI

    Plasmid
    #176333
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617.1 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176333-plasmid-map-sequence-id-347142

    Enlarge

  6. Desmoplakin II donor only control (FL-based no force control)

    Plasmid
    #118722
    Purpose
    The donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y72L mutation in mCherry…
    Promoter
    CMV
    Available Since
    Dec. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118722-plasmid-map-sequence-id-230466

    Enlarge

  7. Desmoplakin II acceptor only control (F40-based no force control)

    Plasmid
    #118720
    Purpose
    The acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)(Y67G)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y67G mutation in YPet(sh…
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118720-plasmid-map-sequence-id-230457

    Enlarge

  8. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Synthetic, Human)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

    Enlarge

  9. pVRC8400-SARS-CoV-2-RBD-SD1-B.1.1.529-AVI

    Plasmid
    #181704
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.529 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181704-plasmid-map-sequence-id-354499

    Enlarge

  10. pVRC8400-SARS-CoV-2-RBD(aa330-526)-B.1.1.529-AVI

    Plasmid
    #182930
    Purpose
    Expresses SARS-CoV-2 RBD (aa324-526) domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD(aa324-526)-B.1.1.529 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    182930-plasmid-map-sequence-id-358143

    Enlarge

  11. pVRC8400-SARS-CoV-2-RBD(aa335-526)-B.1.1.529-AVI

    Plasmid
    #181701
    Purpose
    Expresses SARS-CoV-2 RBD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-B.1.1.529 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181701-plasmid-map-sequence-id-354500

    Enlarge

  12. pVRC8400-SARS-CoV-2-NTD-B.1.1.529-AVI

    Plasmid
    #181698
    Purpose
    Expresses SARS-CoV-2 NTD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-NTD-B.1.1.529 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    A67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181698-plasmid-map-sequence-id-354496

    Enlarge

  13. Desmoplakin II donor only control (F40-based tension sensor)

    Plasmid
    #118717
    Purpose
    The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118717-plasmid-map-sequence-id-230455

    Enlarge

  17. pVRC8400-SARS-CoV-2-RBD-SD1-BA.2-AVI

    Plasmid
    #184534
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-BA.2 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …
    Available Since
    June 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    184534-plasmid-map-sequence-id-364434

    Enlarge

  19. pVRC8400-SARS-CoV-2-RBD-SD1-K417N-L452R-T478K-AVI

    Plasmid
    #176457
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-K417N-L452R-T478K (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    K417N, L452R, T478K
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176457-plasmid-map-sequence-id-347378

    Enlarge

  20. pVRC8400-SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y-AVI

    Plasmid
    #176450
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176450-plasmid-map-sequence-id-347372

    Enlarge

  21. pVRC8400-SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y-AVI

    Plasmid
    #176451
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing K417T-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    K417T, E484K, N501Y
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176451-plasmid-map-sequence-id-347374

    Enlarge

  22. pVRC8400-SARS-CoV-2-S2P-B.1.617-E154K-L452R-E484Q-D614G-P681R-AVI

    Plasmid
    #176331
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing E154K-L452R-E484Q-D614G-P681R mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    E154K, L452R, E484Q, D614G, P681R
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176331-plasmid-map-sequence-id-347140

    Enlarge

  27. pVRC8400-SARS-CoV-2-RBD-XBB.1.5-AVI

    Plasmid
    #198466
    Purpose
    Expresses SARS-CoV-2 RBD protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-XBB.1.5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339H, R346T, L368I, S371F, S373P, S375F, T376A, …
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198466-plasmid-map-sequence-id-388113

    Enlarge

  28. pVRC8400-SARS-CoV-2-S2P-B.1.1.529-AVI

    Plasmid
    #181695
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.529 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    A67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181695-plasmid-map-sequence-id-354495

    Enlarge

  32. pVRC8400-SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D-AVI

    Plasmid
    #176332
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176332-plasmid-map-sequence-id-347141

    Enlarge

  33. pVRC8400-SARS-CoV-2-RBD-L455RA475RG502R-AVI

    Plasmid
    #160480
    Purpose
    Expresses SARS-CoV-2 RBD-L455RA475RG502R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:
    Insert
    SARS-CoV-2-RBD-L455RA475RG502R
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    changed Leucine 455 to Arginine, Alanine 475 to A…
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160480-plasmid-map-sequence-id-314491

    Enlarge

  37. pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVI

    Plasmid
    #198465
    Purpose
    Expresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-XBB.1.5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198465-plasmid-map-sequence-id-388112

    Enlarge

  46. pEMS1401

    Plasmid
    #29198
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available Since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29198-plasmid-map-sequence-id-13613

    Enlarge

  48. pEMS1538

    Plasmid
    #29261
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available Since
    Oct. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29261-plasmid-map-sequence-id-13676

    Enlarge

  49. pJTB126

    Plasmid
    #248910
    Purpose
    expresses the yeast analog-sensitive Smk1-Q120A and Ssp2 fused to glutathione-S-transferase in bacteria
    Depositor
    Edward Winter
    Article
    Rimal et al Mol Cell Biol. 2020 Jul 29;40(16). pii:
    Inserts
    SMK1 Mitogen-activated Protein Kinase -analog-sensitive form (SMK1 Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes from S288C form, Budding Yeast)
    Ssp2 Activator of the Smk1 MAPK (SSP2 Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form, Budding Yeast)
    Tags
    glutathione-S-transferase
    Expression
    Bacterial
    Mutation
    codon 120 has been changed from Q to A (Smk1-Q120…
    Promoter
    T7
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248910-plasmid-map-sequence-id-497716

    Enlarge

Showing: 6601 - 6650 of 15549 results