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pKeratin-Electra2

Plasmid

#184928

Purpose

Keratin labeling with blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Keratin-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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p347_DG

Plasmid

#234677

Purpose

Golden gate domesticated pET28b with His10-Thrombin

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-Thrombin

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3-KASH5-DN

Plasmid

#171492

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

KASH5-DN (CCDC155 B. tarus (bovine))

Use

Pgehme

Tags

mClover3

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-GST-KASH5-DN

Plasmid

#171493

Purpose

T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

KASH5-DN (CCDC155 B. tarus (bovine))

Use

Pgehme

Tags

GST

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRepair-MPP8_mAID-P2A-BFP

Plasmid

#175036

Purpose

Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

mAID-P2A-BFP

Use

Hdr donor template

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FAM20C:V5

Plasmid

#63584

Purpose

FAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.

Depositor

Kenneth Irvine

Article

Ishikawa et al PLoS One. 2012;7(8):e42988. doi: 10.1371

Insert

FAM20C (Fam20c Mouse)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223_ERRFI1_WT

Plasmid

#82116

Purpose

Gateway Donor vector containing ERRFI1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERRFI1 (ERRFI1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAPE

Plasmid

#217451

Purpose

For digestion with restriction enzymes to create DNA size marker ladders

Depositor

Michael Lardelli

Article

Allen et al Biol Methods Protoc. 2023 Jul 31;8(1):bp

Type

Empty backbone

Use

Dna size markers

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 NHE-RF

Plasmid

#11635

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

NHE-RF (NHERF1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
JAM3-bio-His

Plasmid

#51807

Purpose

Expresses full-length Junctional adhesion molecule C precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

JAM3 (JAM3 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPAkt

Plasmid

#181914

Purpose

Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

Insert

InPAkt

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

Plasmid

#196829

Purpose

Express dpdpCas13b with AAAA mutation and Clover

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

dpspCas13b(AAAA)

Tags

3XFLAG and NES

Expression

Mammalian

Mutation

K367A, D369A and K370A

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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mOrange2-Peroxisomes-2

Plasmid

#54596

Purpose

Localization: Peroxisomes, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

mOrange2-Peroxisomes

Tags

Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pME Actin Vhh Halo Tag (JDW 1223)

Plasmid

#224498

Purpose

Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-Halo-Tag

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Plastin-C-10

Plasmid

#55438

Purpose

Localization: Fimbrin/Cytoskeleton, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Plastin (PLS1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS11D-puro

Plasmid

#158475

Purpose

Constitutive lentiviral expression of human TMPRSS11D.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS11D (TMPRSS11D Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST- IP2.0(codon for C.elegans)

Plasmid

#109216

Purpose

IP2.0 for C. elegans (Codon optimization and introns).

Depositor

Takeshi Ishihara

Article

Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

Insert

IP2.0 (Inverse Pericam 2.0)

Tags

His-tag

Expression

Worm

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5-FLAG-Gene33

Plasmid

#21633

Depositor

John Kyriakis

Article

Makkinje et al J Biol Chem. 2000 Jun 9. 275(23):17838-4

Insert

Gene33 (Errfi1 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pET-S9

Plasmid

#132948

Purpose

Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S9 (rpsI E.coli)

Tags

N-terminal 6xHis-tag

Expression

Bacterial

Mutation

E52C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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