159,258 results

1. APEX2-NLS

   Plasmid

   #124617

   Purpose

   proximity biotinylation

   Depositor

   Alice Ting

   Article

   Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

   Insert

   APEX2-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_tre_dCas9_KRAB

   Plasmid

   #126030

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with KRAB
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-OptoSTIM1

   Plasmid

   #70159

   Purpose

   Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

   Depositor

   Won Do Heo

   Article

   Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

   Insert

   OptoSTIM1 (STIM1 Human, Mustard Weed)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_AGIA-AirID

   Plasmid

   #182210

   Purpose

   Express AirID in mammalian cells.

   Depositor

   Sohei Ito, Akira Nozawa, Tatsuya Sawasaki, Shogo Nakano

   Article

   Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

   Insert

   AGIA-AirID
   

   Use

   Tags

   AGIA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCre

   Plasmid

   #236212

   Purpose

   Arabinose-inducible Cre recombinase

   Depositor

   Matthew Waldor

   Article

   Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

   Insert

   Cre recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Cre

   Plasmid

   #123133

   Purpose

   Mammalian expression vector for wild-type Cre.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Cre recombinase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. SrtA-PDGFR Tm

   Plasmid

   #121168

   Purpose

   pMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR Tm

   Depositor

   Gabriel Victora

   Article

   Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

   Insert

   SrtA-PDGFR
   

   Use

   Retroviral

   Tags

   Tomato and flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TRE-dCas9-VPR

   Plasmid

   #63800

   Purpose

   SP-dCas9-VPR with doxycycline-inducible expression

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETa mEos2

   Plasmid

   #20341

   Purpose

   bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

   Depositor

   Loren Looger

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   mEos-2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_SETDB1_WT

    Plasmid

    #82291

    Purpose

    Gateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SETDB1 (SETDB1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PE2

    Plasmid

    #132775

    Purpose

    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HA-HIF1alpha-wt-pBabe-puro

    Plasmid

    #19365

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt human cDNA

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

    Plasmid

    #192617

    Purpose

    AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    FLEX-RiboL1-jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-mTH-GFP

    Plasmid

    #99128

    Purpose

    An AAV genome that expresses GFP from the mouse TH promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mTH

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWPI-N1ICD

    Plasmid

    #185525

    Purpose

    Constitutive overexpression of human NOTCH1 intracellular domain

    Depositor

    Eric Shusta, Sean Palecek

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

    Plasmid

    #126705

    Purpose

    donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CL20_mEGFP_RUNX1_RUNX1T1

    Plasmid

    #205915

    Purpose

    Express mEGFP-tagged fusion protein, RUNX1_RUNX1T1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    RUNX1_RUNX1T1 (RUNX1, RUNX1T1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-6xHis-SUMO-MarathonRT

    Plasmid

    #109029

    Purpose

    Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

    Depositor

    Anna Pyle

    Article

    Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

    Insert

    ERE_01490
    

    Use

    Tags

    6xHis-SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHyperTEV60

    Plasmid

    #228496

    Purpose

    10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

    Depositor

    Richard Baker

    Insert

    TEV Protease
    

    Use

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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