We narrowed to 4,269 results for: Bre;
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Plasmid#164832PurposemiHiFiCas9 expressing plasmidDepositorInsertmiHiFiCas9 (contains Brex27 at sequence 5031-5138).
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationchange R 691 to APromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmiCas9
Plasmid#162758Purposeexpress miCas9DepositorInsertmiCas9
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationContains Brex27 sequence range is 4958-5065PromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gαi*-BERKY3
Plasmid#158424PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsmycExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Actin Binding Domain Tensionless Control
Plasmid#215371PurposeExpresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).DepositorInsertActin Binding Domain Tensionless Control
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSc-humanADAR2
Plasmid#171106PurposeExpresses human ADAR2 in yeast.DepositorInserthuman ADAR2 (ADARB1 Human)
UseTagsHis12_TEVExpressionYeastMutationPromoterGAL1Available sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (S939A/T1462A)
Plasmid#14131DepositorInsertTSC2 S939A/T1462A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationS939A/T1462APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (S939A)
Plasmid#14132DepositorInsertTSC2 S939A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationS939A.PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK7 T194D
Plasmid#80886Purposemammalian expression of ALK7 T194DDepositorInsertALK7 (Acvr1c Rat)
UseTagsHAExpressionMammalianMutationT194D (constitutively active)PromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYB2 reporter
Plasmid#197276PurposeEncodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Ash2L full
Plasmid#15549DepositorInsertAsh2L (ASH2L Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (T1462A)
Plasmid#14130DepositorInsertTSC2 T1462A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationT1462APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 G183S ONE-GO
Plasmid#204338PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3 G183S ONE-GO
UseLentiviralTagsExpressionMutationG183S mutation in Galpha-i3 (renders it RGS insen…PromoterAvailable sinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-ALK7 K222R
Plasmid#80887Purposemammalian expression of ALK7 K222RDepositorInsertALK7 (Acvr1c Rat)
UseTagsHAExpressionMammalianMutationK222RPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS7
Plasmid#111764PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gβγ-BERKY1
Plasmid#158429PurposeBRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK3ct
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
VAMP2-12aa-mCit
Plasmid#53237PurposeFluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags12aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS5
Plasmid#111763PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTag-P2A-QFGal4-SV40pA
Plasmid#155127PurposeDonor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld methodDepositorInsertP2A-QFGal4
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
p40phox-PX (1-144)
Plasmid#119123PurposeBacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)DepositorInsertp40phox-PX (1-144)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VinculinI997A_TurboID_Venus
Plasmid#200274PurposeExpressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculinI997A_TurboID_Venus
UseTagsExpressionMammalianMutationchange isoleucine to alanine at 997 of vinculinPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY3
Plasmid#158428PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsMycExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-hSyn-Gαq*-BERKY1
Plasmid#158430PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK2(RH)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)
Plasmid#117908PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p47phox-PX (1-122)
Plasmid#119124PurposeBacterial expression of human phox homology (PX) domain, p47phox-PX (1-122)DepositorInsertp47phox-PX (1-122)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mPLD1-PX (71-210)
Plasmid#119127PurposeBacterial expression of human phox homology (PX) domain, mPLD1-PX (71-210)DepositorInsertmPLD1-PX (71-210)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY1
Plasmid#158427PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-KB1753-myc
UseLentiviralTagsmycExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)
Plasmid#117911PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A & H45Q on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-IDS_DF_C2c_attB_rev
Plasmid#182151PurposeTo insert attB-rev attachment site at IDS in human cells via twinPEDepositorInsertIDS-C2c-attBrev pegRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
Plasmid#117909PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR98A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only