We narrowed to 13,587 results for: TIM

1. pC13N-dCas9-BFP-KRAB

   Plasmid

   #127968

   Purpose

   constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   CLYBL-CAG-dCas9-NLS-BFP-KRAB
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

   Plasmid

   #174007

   Purpose

   Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

   Depositor

   Mark Histed

   Article

   LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

   Has Service

   AAV9

   Insert

   jGCaMP8s-P2A-ChrimsonR-ST
   

   Use

   AAV

   Tags

   6xHis and soma targeting motif

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. VAMP3-mCherry

   Plasmid

   #92423

   Purpose

   Expression of VAMP3 C-terminally conjugated to mCherry.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   VAMP3 (Vamp3 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-mPGK-eGFP (beta-globin intron)

   Plasmid

   #162513

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

   Depositor

   Bart Nieuwenhuis

   Article

   Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mPGK

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJR98

   Plasmid

   #187239

   Purpose

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-ABE7.10

   Plasmid

   #102919

   Purpose

   expresses ABE7.10 in mammalian cells

   Depositor

   David Liu

   Article

   Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

   Insert

   ABE7.10
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-IP- SCAT3.2

   Plasmid

   #172497

   Purpose

   SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.

   Depositor

   David Scadden

   Article

   Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

   Insert

   Amcyan
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   MLV LTR

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-SRRM2-mCherry

   Plasmid

   #174089

   Purpose

   Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

   Depositor

   Katie Ullman, Wesley Sundquist

   Article

   Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

   Insert

   SRRM2 (SRRM2 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available since

   Sept. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458 V3

   Plasmid

   #226957

   Purpose

   CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. tetO.Sox9.Puro

    Plasmid

    #117269

    Purpose

    Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Sox9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A and puromycin resistant cassette (C terminal …

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. tetO.Nfib.Hygro

    Plasmid

    #117271

    Purpose

    Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Nfib (Nfib Mouse)
    

    Use

    Lentiviral

    Tags

    IRES and hygromycin resistant cassette (C termina…

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

    Plasmid

    #110854

    Purpose

    Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    sgRNA scaffold with spacer
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    U6

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. RNASEL gRNA (BRDN0001149358)

    Plasmid

    #77052

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRES proHB-EGF WT

    Plasmid

    #11602

    Depositor

    Rosalyn Adam

    Article

    Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

    Insert

    HB-EGF (HBEGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pSLQ1658-dCas9-EGFP

    Plasmid

    #51023

    Purpose

    Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    dCas9 fuse to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-CjABE8e

    Plasmid

    #189929

    Purpose

    Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    CjABE8e
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CjCas9 D8A

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1/cyto-iAspSnFR

    Plasmid

    #201394

    Purpose

    CMV overexpression of cyto-iAspSnFR in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

    Insert

    cyto-iAspSnFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

    Promoter

    CMV

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-CD28-mCherry

    Plasmid

    #223593

    Purpose

    Lentiviral expression of human CD28 with mCherry tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pYFP-ErbB2

    Plasmid

    #66948

    Purpose

    Mammalian expression of rat ErbB2 tagged with YFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    (See depositor comment below on mutations A24T an…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

22. AAV-CamKII-TRPV1-P2A-mCherry

    Plasmid

    #200829

    Purpose

    Transduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporter

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-mCherry (Trpv1 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. lenti SYN-FLAG-dCas9-VPR

    Plasmid

    #114196

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SYN

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. p35sheLL

    Plasmid

    #40626

    Depositor

    Simon Beddows

    Article

    Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

    Inserts

    HPV35 L1
    HPV35 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression

    Promoter

    CMV and Polio IRES

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMV306DIhsp+LuxG13

    Plasmid

    #49999

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    G13 from M. marinum G13 promoter driving luxC

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pEB2-mScarlet-I

    Plasmid

    #104007

    Purpose

    Plasmid encoding mScarlet-I

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mScarlet-I
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. FGFR1-optoDroplet

    Plasmid

    #111509

    Purpose

    For optogenetic clustering of FGFR1

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    FGFR1 (FGFR1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2, FUS, Fusion Red, and Myristoylation sequenc…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEB2-mScarlet

    Plasmid

    #104006

    Purpose

    Plasmid encoding mScarlet

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mScarlet
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC18T-mini-Tn7T-Gm-Pc-sfGFP

    Plasmid

    #199248

    Purpose

    Fluorescent labelling of bacterial cells with sfGFP

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized sfGFP gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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