We narrowed to 9,346 results for: UTY

1. pPD527 VP16-ZF14

   Plasmid

   #138756

   Purpose

   Constitutive expression of VP16-ZF14 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF14
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLsCas13a-gRNA-NT

   Plasmid

   #164857

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-NT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSB3C5-proC-B0032-E0051

   Plasmid

   #107242

   Purpose

   Expresses lacZa-GFP fusion from constitutive proC promoter

   Depositor

   Robert Sauer, Joseph Davis

   Article

   Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

   Insert

   ProC-RBS(B0032)-Gemini (E0051)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   proC

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSB3C5-proB-B0032-E0051

   Plasmid

   #107243

   Purpose

   Expresses lacZa-GFP fusion from constitutive proB promoter

   Depositor

   Robert Sauer, Joseph Davis

   Article

   Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

   Insert

   ProB-RBS(B0032)-Gemini (E0051)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   proB

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBbB12c-GFP

   Plasmid

   #126127

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23151

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBzCas13b-gRNA-NT

   Plasmid

   #164861

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-NT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBzCas13b-gRNA-1

   Plasmid

   #164858

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBbA11a-RFP

   Plasmid

   #126084

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23150

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJUMP19-Pbad_P

   Plasmid

   #127003

   Purpose

   Basic Part P- Promoter (non-constitutive); Pbad (I13453), arabinose induced; controlled by AraC.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part Pbad
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJUMP19-T7pro_P

    Plasmid

    #127002

    Purpose

    Basic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymerase

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part T7 promoter_P
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK_proK6_eGFP

    Plasmid

    #59850

    Purpose

    Constitutively expresses eGFP with proK6 promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    S2R,S65G,S72A

    Promoter

    proK6

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFGL1010

    Plasmid

    #119081

    Purpose

    Introducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).

    Depositor

    Naweed Naqvi

    Article

    Patkar et al Nat Chem Biol. 2015 Sep;11(9):733-40. do

    Type

    Empty backbone

    Use

    Fungal expression (in magnaporthe oryzae)

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. piHGpd/p82

    Plasmid

    #1105

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wild-type HSP82 regulated by the strong constitut…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

14. piHGpd/G170D

    Plasmid

    #1113

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HSP82 with glycine 170 changed to aspartic acid; …

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pMM725

    Plasmid

    #68895

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL3), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBbS18c-RFP

    Plasmid

    #126201

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23117

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJUMP19-J23107_P

    Plasmid

    #127000

    Purpose

    Basic Part P- Promoter (constitutive); J23107.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part J23107_P
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJUMP19-J23100_P

    Plasmid

    #126996

    Purpose

    Basic Part P- Promoter (constitutive); J23100

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part J23100_P
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBbS10a-RFP

    Plasmid

    #126173

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23116

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbB18k-GFP

    Plasmid

    #126137

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23117

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBbB14c-GFP

    Plasmid

    #126129

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBbB13c-GFP

    Plasmid

    #126128

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMM750

    Plasmid

    #68899

    Purpose

    IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBbS15a-RFP

    Plasmid

    #126190

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23100

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUASp YFP Rab23 CA

    Plasmid

    #37728

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab23 (Rab23 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q96L (constitutive active)

    Promoter

    GAL4

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pOGG203

    Plasmid

    #115504

    Purpose

    Clone of a level 1 unit which encodes a constitutively-expressed mCherry

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    pL1M-F2-pNeo pOGG001, mCherry EC15071, T-pharma(pOGG003
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMM732

    Plasmid

    #68897

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p2HGAL/CGR/CYC

    Plasmid

    #1218

    Depositor

    Susan Lindquist

    Article

    Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

    Insert

    CGR (Nr3c1 Rat)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    pRS423 with GAL promoter and CYC terminator gene…

    Promoter

    Available since

    July 12, 2006

    Availability

    Academic Institutions and Nonprofits only

29. P16ΔS:(MCS)-S11

    Plasmid

    #108184

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    P16ΔS

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pL-rpb1-C67Y

    Plasmid

    #10982

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-C67Y (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 200 G to A in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

31. pL-rpb1-C70Y

    Plasmid

    #10983

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-C70Y (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 209 G to A in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

32. pL-rpb1-H80Y

    Plasmid

    #10984

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-H80Y (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 238 C to T in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

33. pJK_pro6_eGFP

    Plasmid

    #59851

    Purpose

    Constitutively expresses eGFP with pro6 promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    S2R,S65G,S72A

    Promoter

    pro6

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJK_proK14_eGFP

    Plasmid

    #59848

    Purpose

    Constitutively expresses eGFP with proK14 promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    S2R,S65G,S72A

    Promoter

    proK14

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUAST YFP mouse Rab35 CA

    Plasmid

    #46015

    Depositor

    Matthew Scott

    Article

    Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

    Insert

    Rab35 (Rab35 Mouse)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q67L (constitutively active)

    Promoter

    GAL4

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

36. piHGpd/T101I

    Plasmid

    #1112

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HSP82 with threonine 101 changed to isoleucine; r…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

37. pL-rpb1-D260N

    Plasmid

    #10985

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-D260N (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 778 G to A in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

38. pL-rpb1-D261N

    Plasmid

    #10986

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-D261N (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 781 G to A in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

39. pL-rpb1-R320C

    Plasmid

    #10987

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-R320C (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 958 C to T in the RPB1 ORF resulting i…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

40. pL-rpb1-E1230K

    Plasmid

    #10990

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-E1230K (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 3688 G to A in the RPB1 ORF resulting in…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

41. pMS09

    Plasmid

    #230073

    Purpose

    Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal iLOV fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

    Depositor

    Sean Crosson

    Article

    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    3xFLAG and iLOV

    Expression

    Bacterial

    Mutation

    Promoter

    RpoD (bt_1311/BT_RS06635)

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMS10

    Plasmid

    #230074

    Purpose

    Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal mNeonGreen fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

    Depositor

    Sean Crosson

    Article

    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    3xFLAG and mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    RpoD (bt_1311/BT_RS06635)

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMS03

    Plasmid

    #230937

    Purpose

    Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

    Depositor

    Sean Crosson

    Article

    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    RpoD (bt_1311/BT_RS06635)

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMS02

    Plasmid

    #230066

    Purpose

    Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

    Depositor

    Sean Crosson

    Article

    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    RpoD (bt_1311/BT_RS06635)

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSEVA26_sgRNA-A0_M13ps

    Plasmid

    #231413

    Purpose

    M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Insert

    sgRNA-A0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pP4-C

    Plasmid

    #225097

    Purpose

    gfpUV reporter vector with a high performance adenine-dependent riboswitch

    Depositor

    Robert Batey

    Article

    Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

    Insert

    synthetic riboswitch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSEVA47_dCas9-GFP_YES_i2

    Plasmid

    #231419

    Purpose

    This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    dCas9
    GFP
    sgRNA-Y
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pSEVA47_dCas9-GFP_NOT_i1

    Plasmid

    #231416

    Purpose

    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    dCas9
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pPOM024_P2_pMPA1

    Plasmid

    #216449

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter mpa1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pPOM022_P2_pRPS1002

    Plasmid

    #216447

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter rps1002
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge