We narrowed to 29,636 results for: Ide

1. CMV Flag PAI-RBP

   Plasmid

   #45452

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP (SERBP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pOPO685

   Plasmid

   #195300

   Purpose

   pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   McCAST Cascade and native array with PaqCI entry site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose inducible arapBAD

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1a-nSpCas9

   Plasmid

   #171691

   Purpose

   Expresses nSpCas9 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   nSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF1a

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. bdSUMO-HA2-GFP-HiBit

   Plasmid

   #171017

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-HA2-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSP2372

   Plasmid

   #73196

   Purpose

   Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, and Q926A in SpCas9

   Promoter

   CMV

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet23a_RBPMS_FL

   Plasmid

   #59392

   Purpose

   Plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HeFm2SpCas9

   Plasmid

   #92111

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   “Highly enhanced Fidelity” mut2 SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIneoLuc-PP1A

   Plasmid

   #128348

   Purpose

   Vector for LUMIER assay

   Depositor

   Yutaka Hata

   Article

   Kawano et al J Biochem. 2015 May 14. pii: mvv051.

   Insert

   PP1A (PPP1CA Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. bdSUMO-pHD118-GFP-HiBit

   Plasmid

   #171015

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-pHD118-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PKM-IRES-hrGFP

    Plasmid

    #73405

    Purpose

    Mammalian expression of mouse PKM and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKM (Prkcz Mouse)
    

    Use

    Tags

    3xFLAG and pIRES-hrGFP-2a

    Expression

    Mammalian

    Mutation

    truncated, constitutively active form

    Promoter

    CMV

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. DsRed +9e

    Plasmid

    #191766

    Purpose

    Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    DsRed
    

    Use

    Tags

    N terminal fusion of positively charged polypetod…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET23a_mod_RBPMS_FL

    Plasmid

    #59393

    Purpose

    Plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    6xHis, FLAG, and HA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. bdSUMO-pHlip-GFP-HiBit

    Plasmid

    #171016

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-pHlip-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pet23a_RBPMS_1_101

    Plasmid

    #65728

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-101

    Promoter

    T7

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-X1-Neo-HA-UFL1-RAMP4

    Plasmid

    #139863

    Purpose

    Lentiviral expression of fusion of human UFL1 (full length) with RAMP4

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    UFL1-RAMP4 (UFL1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    recombinant fusion of UFL1 (full length) with RAM…

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET23a_mod_RBPMS2_FL

    Plasmid

    #59394

    Purpose

    Plasmid for bacterial overexpression of RBPMS2

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Use

    Tags

    6xHis, FLAG, HA, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDM358_DPAKa(GBD)-YFP

    Plasmid

    #128700

    Purpose

    expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

    Insert

    GBD domain (aa 731-890) of DPAKa
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP

    Expression

    Mutation

    none

    Promoter

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pet28a_RBPMS_1_129

    Plasmid

    #65738

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-129

    Promoter

    T7

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pet28a_RBPMS_1_100

    Plasmid

    #65735

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-100

    Promoter

    T7

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR4-RBPMS

    Plasmid

    #59387

    Purpose

    Entry vector with RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge