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We narrowed to 12,214 results for: LEA

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Showing: 2661 - 2680 of 12214 results

  7. pResQ shFEN3 3XF-FEN1 wt

    Plasmid
    #17752
    Depositor
    Sheila Stewart
    Insert
    Flap Endonuclease I (FEN1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Triple Flag
    Expression
    Mammalian
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  9. GFP-DroshaS300/302A

    Plasmid
    #62530
    Purpose
    Serines 300 and 302 of Drosha are mutated to alanines
    Depositor
    Bharat Ramratnam
    Publication
    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    changed serines 300 and 302 to alanines
    Available since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. SpCas9-ABE8e(V106W)

    Plasmid
    #198547
    Purpose
    Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198547-plasmid-map-sequence-id-388882

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  11. SpCas9-NG-YE1-BE4max

    Plasmid
    #198535
    Purpose
    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198535-plasmid-map-sequence-id-388892

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  12. pET28-AviTag-DogCatcher-MBP

    Plasmid
    #171928
    Purpose
    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Publication
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogCatcher-MBP
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    DogCatcher added to N-terminus of MBP via a GSGES…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171928-plasmid-map-sequence-id-338570

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  13. pDN41

    Plasmid
    #61344
    Purpose
    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)
    Depositor
    Barbara Di Ventura, Roland Eils
    Publication
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    GS linker-AsLov2-biNLS9 and PKIt NES
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61344-plasmid-map-sequence-id-102122

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  14. CBX2

    Plasmid
    #25158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX2_04 (CBX2 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    25158-plasmid-map-sequence-id-472908

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  15. pCDNA3 V5 mDispatched WT 2x HA

    Plasmid
    #126410
    Purpose
    Expression of V5 Dispatched WT HA in mammalian cell lines.
    Depositor
    Stacey Ogden
    Publication
    Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    m-Dispatched1 (Disp1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    V5 iserted between A106 and A107
    Available since
    June 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126410-plasmid-map-sequence-id-246847

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  16. pALS099 (pGEX-2TP-cTevHis-DEST)

    Plasmid
    #139787
    Purpose
    Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis
    Depositor
    Jeannie Lee, Arneet Saltzman
    Publication
    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:
    Type
    Empty backbone
    Tags
    6xHis (cleavable by TEV protease) and GST (cleava…
    Expression
    Bacterial
    Available since
    April 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139787-plasmid-map-sequence-id-325052

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  17. pOCC304

    Plasmid
    #118897
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with TEVprotease and TwinStrep-MB…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118897-plasmid-map-sequence-id-232369

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  19. pDisplay-GRAB_OT1.0

    Plasmid
    #185384
    Purpose
    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells
    Depositor
    Yulong Li
    Publication
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based oxytocin sensor GRAB_OT1.0
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    185384-plasmid-map-sequence-id-366026

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  20. 2xFKBP-mCh-KDEL

    Plasmid
    #173010
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2xFKBP-mCh-KDEL
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173010-plasmid-map-sequence-id-339203

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Showing: 2661 - 2680 of 12214 results