We narrowed to 8,094 results for: gnal

1. Dox-SOScat

   Plasmid

   #207329

   Purpose

   PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)

   Depositor

   Jared Toettcher

   Article

   Underhill et al Development. 2023 Aug 15;150(16):dev2016

   Insert

   son of sevenless homolog 2 variant, catalytic domain
   

   Use

   Tags

   myristoylation tag - BFP

   Expression

   Bacterial

   Mutation

   range: 593 to 1078

   Promoter

   Tet Response Element

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL0_DE-Per_sig_pep

   Plasmid

   #202152

   Purpose

   Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   Peroxisome signal peptide
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Oct. 19, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. GFP hSmad4-HomDonor

   Plasmid

   #206044

   Purpose

   Donor plasmid for CRISPR knock-in

   Depositor

   Ali Hemmati Brivanlou

   Article

   Nemashkalo et al Development. 2017 Sep 1;144(17):3042-305

   Insert

   Smad4 (SMAD4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. NbV5-TEV219S153N

   Plasmid

   #201488

   Purpose

   TANGO detection of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-TEV219S153N
   

   Use

   Tags

   TEV219 S153N

   Expression

   Mammalian

   Mutation

   S153N in TEV219

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TagBFP2-Mss4D636K-Kina

   Plasmid

   #202725

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Mss4D636K-Kina

   Plasmid

   #202740

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mCherry:S. cerevisiae Mss4 (377-756)(D636K) (MSS4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACT2-μ4

   Plasmid

   #198176

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-4 μ4
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGADT7-αC

   Plasmid

   #197650

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-2 αC
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Ser677T substitution

   Promoter

   ADH1

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACT2-μ2

   Plasmid

   #198173

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-2 μ2
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSyn-ASAP3ER

    Plasmid

    #195358

    Purpose

    ASAP3ER under control of human synapsin promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    ASAP3ER
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMpGE010-Target1 (Mpphot)

    Plasmid

    #186728

    Purpose

    Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

    Depositor

    Yutaka Kodama

    Article

    Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

    Insert

    Mphot
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p8271 LentiCRISPR v2 hygro sgSIGIRR-4

    Plasmid

    #193980

    Purpose

    Expression of spCas9 and sgRNA targeting SIGIRR

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-mVenusC-14-HP1b Chromo

    Plasmid

    #191398

    Purpose

    HP1b chromo domain fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    FLAG-mVenusC-14-HP1b Chromo
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-NLS-MaSat ZF-18-mVenusN

    Plasmid

    #191392

    Purpose

    Zinc finger fused to N-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    Flag-NLS-MaSat ZF-18-mVenusN
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1-mScarlet-AMPKα2

    Plasmid

    #192451

    Purpose

    mScarlet-tagged alpha2 isoform of AMPK catalytic subunit.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    mScarlet-AMPKalpha2 (Prkaa2 Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV hUbC-Cas9-P2A-Puro_ST-sgRNA

    Plasmid

    #188705

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting loci

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    ST sgRNAs
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV103-PRNR2-mCherry-Elk1DM-1SIM

    Plasmid

    #190252

    Purpose

    Expression mCherry-Elk1DM in yeast cells

    Depositor

    Liam Holt

    Article

    Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

    Insert

    ELK1DM-SIM
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV105-PPAB1-GFP-Erk1R84S-1SIM

    Plasmid

    #190253

    Purpose

    Expression Erk1-GFP-1SIM in yeast cells

    Depositor

    Liam Holt

    Article

    Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

    Insert

    ERK1R84S-SIM
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    ERK1R84S

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMpGE010-Target2 (Mpphot)

    Plasmid

    #186729

    Purpose

    Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

    Depositor

    Yutaka Kodama

    Article

    Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

    Insert

    Mphot
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p-preSufI(IAC)

    Plasmid

    #168516

    Purpose

    Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    pre-SufI(IAC)
    

    Use

    Tags

    6xHisC tag and HSV tag

    Expression

    Bacterial

    Mutation

    C17I and C295A

    Promoter

    T7

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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