59,474 results

1. GFP-polyQ74-NLS

   Plasmid

   #231892

   Purpose

   Forms nuclear HTT partial exon 1 Q74-NLS aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74-NLS (HTT Human)
   

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. TJP1_PDZ_1

   Plasmid

   #103909

   Purpose

   Protein expression and purification of ZO1 PDZ1 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   TJP1 (TJP1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. nLuc-GLP-1R-His-FLAG

   Plasmid

   #124831

   Purpose

   Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells

   Depositor

   Sam Gellman

   Article

   Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. d

   Insert

   GLP1R (GLP1R Synthetic, Human)
   

   Use

   Luciferase

   Tags

   FLAG, His6, and NanoLuc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1a-GFP-SFPQY527A-IRES-mCherry

   Plasmid

   #166951

   Purpose

   Lentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ-Y527A (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   GFP and mCherry

   Mutation

   SFPQ-Y527A

   Promoter

   eF1a

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-C1-Nir1-LNS2

   Plasmid

   #220084

   Purpose

   high affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Tags

   mCherry-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP.N3-SLC35A1-GFP

   Plasmid

   #186281

   Purpose

   transient expression of C-terminal GFP tagged SLC35A1 isoform a in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

   Insert

   SLC35A1 (SLC35A1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK1253

   Plasmid

   #133058

   Purpose

   Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.

   Depositor

   Martin Kampmann

   Article

   Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

   Insert

   Tau.K18(LM)
   

   Use

   Lentiviral

   Tags

   mClover2

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. px458_2A_GFP_sgRNA_LTN1

   Plasmid

   #127125

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA LTN1 (LTN1 Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-GFP-AURKA-mCherry

   Plasmid

   #99876

   Purpose

   Expression of GFP-AURKA-mCherry for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p414-TEF1p-Cas9-CYC1t

    Plasmid

    #43802

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGST1-TOM1-VHS

    Plasmid

    #44423

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

    Insert

    TOM1-VHS (1-152) (TOM1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

12. plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA

    Plasmid

    #218527

    Purpose

    sgRNA targeting human EIF2AK2/PKR

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI-Puro_hCOL4A2-Myc-His-WPRE

    Plasmid

    #198080

    Purpose

    Express hCOL4A2 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A2 (COL4A2 Human)
    

    Tags

    TEV-Myc-His

    Expression

    Mammalian

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCLX-PGK-hTERT

    Plasmid

    #114315

    Purpose

    Constitutive expression of the hTert protein

    Depositor

    Patrick Salmon

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N1 alpha-actinin 1

    Plasmid

    #11908

    Depositor

    Carol Otey

    Article

    Edlund et al Cell Motil Cytoskeleton. 2001 Mar . 48(3

    Insert

    alpha-actinin 1 (ACTN1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGFP Cortactin

    Plasmid

    #50728

    Purpose

    mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Cortactin (CTTN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. DLG1_PDZ_3

    Plasmid

    #103917

    Purpose

    Protein expression and purification of DLG1 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG1 (DLG1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. KRAB-dCas9

    Plasmid

    #112195

    Purpose

    KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    KRAB (ZNF10 Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-KRAB-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. DLG1_PDZ_2

    Plasmid

    #103916

    Purpose

    Protein expression and purification of DLG1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG1 (DLG1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DLG1_PDZ_1

    Plasmid

    #103915

    Purpose

    Protein expression and purification of DLG1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG1 (DLG1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge