We narrowed to 34,434 results for: CaS;

1. pXD71Cas10.0-Pct5.1-crRNA(AarI)

   Plasmid

   #191633

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-NRCH-YE1-BE4max

   Plasmid

   #198549

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-EF1alpha-dCas9-p300_Blast

   Plasmid

   #192653

   Purpose

   3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKRG3-mU6-PUb-hSpCas9

   Plasmid

   #162162

   Purpose

   Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   N/A

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-TRE3G-dCas9-HaloTag

   Plasmid

   #169448

   Purpose

   expression of dCas9-HaloTag

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   dCas9-HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0043-PspCas13b-gRNA LINE1

   Plasmid

   #223699

   Purpose

   gRNA of dPspCas13b-FTO targeting LINE1

   Depositor

   Chuan He

   Article

   Wei et al Science. 2022 May 27;376(6596):968-973.

   Insert

   gRNA target sequence for LINE1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHLS-EF1a-FRB-SpCas9-A

   Plasmid

   #138477

   Purpose

   Expresses FRB fused SpCas9 for NanoMEDIC packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   SpCas9, human codon optimized
   

   Use

   CRISPR

   Tags

   3x HA tag and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1- dCas9-2xNLS-EGFP

   Plasmid

   #74710

   Purpose

   Expresses dCas9-2xNLS-EGFP in mammalian cells

   Depositor

   Eugene Yeo

   Article

   Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

   Insert

   dCas9-2xNLS-EGFP
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2T-CMV-evoCjCas9-BlastR

   Plasmid

   #194059

   Purpose

   Mammalian expression of evoCjCas9 nuclease

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB1619 hU6-BpiI-Cas13bt1-DR

    Plasmid

    #176314

    Purpose

    hU6-BpiI-Cas13bt1-DR (crRNA expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    Cas13bt1 crRNA direct repeat
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NRCH-ABE8e(V106W)

    Plasmid

    #198556

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. BICstim-Gag-dCAS9-VPR

    Plasmid

    #120922

    Purpose

    encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

    Depositor

    Emiliano Ricci, Philippe Mangeot, Théophile Ohlmann

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GAG (FMLV)-dCAS9-VPR
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-fFuCas9-HTBNLS-hph

    Plasmid

    #121092

    Purpose

    Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

    Depositor

    Xiao-Jun Ji

    Article

    Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

    Insert

    Cas9 and hygromycin resist gene
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    gpdA;trpC

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. px335-sgRNA-EF(Cas9n)-Dnmt3b-R

    Plasmid

    #122333

    Purpose

    Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Dnmt3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-dCas13-inactive M3nls

    Plasmid

    #157854

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZLCv2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106357

    Purpose

    Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB[vasa-Cas9 PUb-ZsGreen]

    Plasmid

    #169012

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster vasa

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. px335-sgRNA-EF(Cas9n)-Dnmt3b-L

    Plasmid

    #122334

    Purpose

    Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Dnmt3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBT/EFS-Cas9-PuroTk

    Plasmid

    #170542

    Purpose

    Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    PuroTK
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-FLEX-SaCas9-U6-sgGrin1

    Plasmid

    #124852

    Purpose

    Mutagenesis of Grin1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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