We narrowed to 7,568 results for: Lif

1. UAS-dsarm(E,K450R)

   Plasmid

   #187881

   Purpose

   Gal4/UAS expression of dSarm(isoE,K450R)

   Depositor

   Lukas Neukomm

   Article

   Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

   Insert

   dSarm isoform E (Sarm )
   

   Use

   Tags

   Expression

   Mutation

   K450R

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP

   Plasmid

   #190039

   Purpose

   Expression of FIP200 mutant

   Depositor

   James Hurley

   Article

   Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Tags

   GST and MBP

   Expression

   Mammalian

   Mutation

   (641-779)deletion & 1363 RDKDLIES 1370 to GSS…

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUGW-shRIIα

   Plasmid

   #183454

   Purpose

   sgRNA targeting rat PKA-RIIα subunits

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA targeting rat PKA-RIIα (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. p8322 pDONR ATG-YAP1 S94A-Stop

   Plasmid

   #184526

   Purpose

   Subcloning YAP1 S94A

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

   Insert

   YAP1 S94A (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   S94A

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p8321 pDONR ATG-YAP1 S127A-Stop

   Plasmid

   #184525

   Purpose

   Subcloning YAP1 S127A

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

   Insert

   YAP1 S127A (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   S127A

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJET-50-DExogron-mCherry-R11b

   Plasmid

   #179904

   Purpose

   Donor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJET-23-DExCon-mCherry-R11b

   Plasmid

   #179898

   Purpose

   Donor with homologous arms for Rab11b to knock in DExCon-mCherry module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJET-67-DExCon-mNeonGreen-R11b

   Plasmid

   #179899

   Purpose

   Donor with homologous arms for Rab11b to knock in DExCon-mNeonGreen module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJET-59-DExCon-mTagBFP2-R25

   Plasmid

   #179900

   Purpose

   Donor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB25, member RAS oncogene family (RAB25 Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mTagBFP2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-PD-1 (BTLA ITIM)-mGFP

    Plasmid

    #180817

    Purpose

    Expression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

    Insert

    PD-1 (BTLA ITIM) (PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    replaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…

    Promoter

    SFFV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX-anti-MIR328-3p

    Plasmid

    #153319

    Purpose

    Expresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.

    Depositor

    Robert Judson-Torres

    Article

    McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

    Insert

    zsGreen with MIR328-3p sponge
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-anti-MIR211-5p

    Plasmid

    #153318

    Purpose

    Expresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.

    Depositor

    Robert Judson-Torres

    Article

    McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

    Insert

    zsGreen with MIR211-5p sponge
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo

    Plasmid

    #179440

    Purpose

    Expresses rTetR-HDAC4(human) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-HDAC4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. iDianiSnFR

    Plasmid

    #177741

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    iDianiSnFR
    

    Use

    Tags

    HA, His, and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-FLAG-APEX2-GABARAPL2

    Plasmid

    #175751

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    GABARAPL2 (GABARAPL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A

    Plasmid

    #175752

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50A

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    GABARAPL2 (GABARAPL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Y49A L50A

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCB’ CsoS2 ΔNTD

    Plasmid

    #162705

    Purpose

    Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deletion of residues 2-256 of CsoS2, Y131D

    Promoter

    PLtet0-1 promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Halo-WIPI2B H85E K88E I92E

    Plasmid

    #175033

    Purpose

    Expresses WIPI2B (H85E K88E I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

    Depositor

    Andrea Stavoe

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2B (H85E K88E I92E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Histadine 85 to Glutamate (CAC to GAG); Lysine 88…

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. iCytSnFR

    Plasmid

    #177738

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    iCytSnFR
    

    Use

    Tags

    HA, His, and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. iCyt_BrEt_SnFR

    Plasmid

    #177740

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    iCyt_BrEt_SnFR
    

    Use

    Tags

    HA, His, and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge