We narrowed to 23,191 results for: his

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PET-HeFSpCas9-NLS-6xHis

Plasmid

#207391

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HeFSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PET-evoSpCas9-NLS-6xHis

Plasmid

#207389

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDL25_Cas9-His Δcys E1207C

Plasmid

#206286

Purpose

Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E1207C

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDL23_Cas9-His Δcys E945C

Plasmid

#206284

Purpose

Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E945C

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDL24_Cas9-His Δcys E1026C

Plasmid

#206285

Purpose

Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E1026C

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDL22_Cas9-His Δcys E532C

Plasmid

#206283

Purpose

Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-K62D/K64D

Plasmid

#202786

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

K62D, K64D

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET24-aCatenin(82-906)-His6

Plasmid

#203332

Purpose

Bacterial expression of wild-type alpha-catenin

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2012 May 25;287(22):18492-9

Insert

CTNNA1 (CTNNA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 82-906

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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Atg11 [699-800] pHis2

Plasmid

#190264

Purpose

Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

Insert

ATG11

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC-Bio2_MVPI_SMAC(delta(55))_9His

Plasmid

#197978

Purpose

E. coli expression of MVPI-SMAC(delta(55))_9His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

Smac (DIABLO Human)

Tags

9His

Expression

Bacterial

Mutation

delta (1-55), A56M

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC-Bio2_SMAC(delta(55))_Cys_9His

Plasmid

#197977

Purpose

E. coli expression of SMAC(delta(55))_Cys-9His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

Smac (DIABLO Human)

Tags

9His

Expression

Bacterial

Mutation

delta (1-55), ins240C

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmXRN1_3xPmut-dsRNAres-V5His6_P

Plasmid

#147205

Purpose

Insect Expression of DmXRN1-3xPmut-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1-3xPmut-dsRNAres (pcm Fly)

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmXRN1_1-573-V5His6_J

Plasmid

#146703

Purpose

Insect Expression of DmXRN1_1-573

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1-573 (pcm Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmXRN1_1-1141-V5His6_J

Plasmid

#146704

Purpose

Insect Expression of DmXRN1_1-1141

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1-1141 (pcm Fly)

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmXRN1_574-1141-V5His6_J

Plasmid

#146705

Purpose

Insect Expression of DmXRN1_574-1141

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_574-1141 (pcm Fly)

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmXRN1_574-1612-V5His6_J

Plasmid

#146706

Purpose

Insect Expression of DmXRN1_574-1612

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_574-1612 (pcm Fly)

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmXRN1_1142-1612-V5His6_J

Plasmid

#146707

Purpose

Insect Expression of DmXRN1_1142-1612

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1142-1612 (pcm Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-V5His6_H

Plasmid

#146499

Purpose

Insect Expression of DmGW182

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-delLN-DmPABPC1-V5His6_H

Plasmid

#146477

Purpose

Insect Expression of DmPABPC1

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1 (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and seven silent mu…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmPABPC1-Nterm-V5His6_H

Plasmid

#146478

Purpose

Insect Expression of DmPABPC1-Nterm

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-Nterm (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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