We narrowed to 5,107 results for: PEP

1. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

   Plasmid

   #106462

   Purpose

   Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(N163,242Q)
   

   Use

   Tags

   PA

   Expression

   Bacterial and Yeast

   Mutation

   changed Asparagines at positions 163 and 242 to G…

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

   Plasmid

   #106466

   Purpose

   Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(S219A)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Changed Serine 219 to Alanine

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]

   Plasmid

   #106467

   Purpose

   Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(H474A)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Changed Histidine 474 to Alanine

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

   Plasmid

   #106468

   Purpose

   Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(D415A)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Changed Aspartate 415 to Alanine

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. csd6_4-330/pET28b(+) N-tag

   Plasmid

   #71351

   Purpose

   csd6, construct:4-330, pET28b(+) N-tag

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 )
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

7. csd6_13-330/pET28b(+) N-tag

   Plasmid

   #71352

   Purpose

   csd6, construct:13-330, pET28b(+) N-tag

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 )
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

8. csd6_1-330/pET28b(+) N-tag

   Plasmid

   #71350

   Purpose

   csd6, construct:1-330, pET28b(+) N-tag

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 )
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

9. SL542

   Plasmid

   #49951

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD RH-8 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

10. SL544

    Plasmid

    #49970

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-10 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

11. SL545

    Plasmid

    #49953

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-11 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

12. SL543

    Plasmid

    #49952

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-9 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

13. SL541

    Plasmid

    #49950

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-7 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

14. SL540

    Plasmid

    #49949

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-6 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCAP03-sp44/p21

    Plasmid

    #120232

    Purpose

    Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    aac(3)IV (apramycin resistance gene)

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)_Granulin 4-no linker

    Plasmid

    #176925

    Purpose

    express Granulin 4 without linker 5 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 4 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid

    #128604

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

    Plasmid

    #199390

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi-SUMOEu

    Expression

    Bacterial

    Mutation

    Promoter

    T5-LacO

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-DYRK1A-2A-GFP

    Plasmid

    #118270

    Purpose

    Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
    

    Use

    AAV

    Tags

    2A peptide and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80062

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Sox10 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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