162,325 results

1. pcDNA3.1(+)Gα15-244-NLuc

   Plasmid

   #207154

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNA15 (GNA15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position E(244)N

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-iPE-Plus

   Plasmid

   #214062

   Purpose

   inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXY027

   Plasmid

   #98915

   Purpose

   Express FtsZ tagged with GFP in E coli

   Depositor

   Jie Xiao

   Article

   Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

   Insert

   FtsZ
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV.CMVenh.gp91.eGFP.cHS4

   Plasmid

   #30471

   Depositor

   Didier Trono

   Article

   Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

   Insert

   CMVenh/gp91/eGFP/cHS4
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-cCFP

   Plasmid

   #185361

   Purpose

   For mammalian expression of C-term CFP for split-CFP

   Depositor

   Ayaz Najafov

   Insert

   CFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDESTtol2pACrymCherry

   Plasmid

   #64023

   Purpose

   Gateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lens

   Depositor

   Joachim Berger, Peter Currie

   Article

   Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

   Insert

   mCherry
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   alpha-Acrystallin promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHygG2

   Plasmid

   #200066

   Purpose

   hygR selection nls-mNG marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PP7_mCherry

   Plasmid

   #61763

   Purpose

   Expresses PP7 coat protein fused to mCherry

   Depositor

   Daniel Larson

   Article

   Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

   Insert

   PP7 coat protein fused to mCherry
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB1 (CK2alpha)

   Plasmid

   #27083

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha (CSNK2A1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-HA-BACE1

    Plasmid

    #165032

    Purpose

    HA-BACE1

    Depositor

    Juan Bonifacino

    Article

    Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

    Insert

    BACE1 (BACE1 Human)
    

    Use

    Tags

    HA (embedded after propeptide cleavage site)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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