165,343 results

1. M13cp-dg3 hp

   Plasmid

   #218095

   Purpose

   Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

   Depositor

   Kevin Esvelt

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

   Plasmid

   #205443

   Purpose

   human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD46-HLA-G SCT-hCD47-hCD59
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-NeoR-CAG-EGFPnls-WPRE

   Plasmid

   #191692

   Purpose

   For knocking pCAG-EGFPnls into AAVS1 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   AAVS1 homology arms and pCAG-EGFPnls
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_FASN

   Plasmid

   #107138

   Purpose

   His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells

   Depositor

   Brijesh Kumar Singh

   Insert

   Fatty acid synthase (FASN Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BoNT/A-LC

   Plasmid

   #31602

   Depositor

   Axel Brunger

   Article

   Zuniga et al Structure. 2008 Oct 8. 16(10):1588-97.

   Insert

   BoNT/A LC
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. tau exon 9–12 splicing minigene

   Plasmid

   #121165

   Purpose

   Generates circular RNAs from a Tau minigene

   Depositor

   Stefan Stamm

   Article

   Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

   Insert

   Tau exons 9–12 (MAPT Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBR1MCS-2

   Plasmid

   #85168

   Purpose

   Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

   Depositor

   Kenneth Peterson

   Article

   Kovach et al Gene. 1995 Dec 1;166(1):175-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Constitutive (derepressed P-lac)

   Available Since

   Nov. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. JUP_pLX307

   Plasmid

   #98347

   Purpose

   Lentiviral expression of JUP

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   JUP (JUP Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GEPIR Sox4.2137

   Plasmid

   #101119

   Purpose

   shRNA Vector

   Depositor

   Joan Massague

   Article

   David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

   Insert

   Sox4.2137
   

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ262-ABE8e

    Plasmid

    #161523

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    ABE8e-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-IRES Hyg SMAD4

    Plasmid

    #107128

    Purpose

    Constitutive Flag-tagged SMAD4 expression in Mammalian cells

    Depositor

    Joan Massague

    Article

    David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

    Insert

    SMAD4 (SMAD4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDA010.EL222

    Plasmid

    #206801

    Purpose

    J23106.EL222

    Depositor

    James Carothers

    Article

    Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

    Insert

    EL222
    

    Promoter

    J23106

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1_Puro_hPGK1_EGFP_Donor

    Plasmid

    #178088

    Purpose

    HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human PGK1

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-GGA3-VHS-GAT

    Plasmid

    #173719

    Purpose

    VHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.

    Depositor

    Juan Bonifacino

    Article

    Dell'Angelica et al J Cell Biol. 2000 Apr 3;149(1):81-94. do

    Insert

    GGA3 (GGA3, GGA2, GGA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pnCas9-miniSdd7-PBE

    Plasmid

    #204859

    Purpose

    For cytosine base editing using miniSdd7 in monocotyledon plants (transient transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    ZmUbi-1

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. iCas

    Plasmid

    #84232

    Purpose

    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

    Depositor

    Meng How Tan

    Article

    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

    Insert

    Cas9
    

    Tags

    2A-OFP co-expression and ERT2-ERT2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHalo-MyosinIIB-C25

    Plasmid

    #128574

    Purpose

    Studies of cellular contractility.

    Depositor

    Dylan Burnette

    Article

    Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

    Insert

    Myosin IIB (MYH10 Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-PFKP

    Plasmid

    #23869

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PFKP (PFKP Human)
    

    Use

    Gateway donor vector

    Mutation

    1MM

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-SARS2SΔC-H2gp41

    Plasmid

    #170389

    Purpose

    Generation of SARS-CoV-2 spike pseudotyped lentivirus

    Depositor

    Nir Hacohen

    Article

    Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2)
    

    Tags

    HIV gp41

    Expression

    Mammalian

    Mutation

    Deletion of C terminal and fusion with H2 from HI…

    Promoter

    CMV

    Available Since

    June 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCMV-Hs.cargo(Cre)

    Plasmid

    #174863

    Purpose

    Expresses human Peg10 cargoRNA encoding Cre recombinase

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    Cre flanked by HsPeg10 UTRs
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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