We narrowed to 6,336 results for: WUS;

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WT-β3

Plasmid

#166579

Purpose

For expression of cytoplasmic tail of mouse beta3 integrin with N-terminal GST fusion protein in bacterial cells.

Depositor

Vesa Hytönen

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

Insert

β3 (Itgb3 Mouse)

Tags

GST

Expression

Bacterial

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B-E2F2

Plasmid

#202521

Purpose

Expressing human E2F2 protein with FLAG-tag

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

E2F2 (E2F2 Human)

Tags

FLAG-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata3

Plasmid

#128824

Purpose

dox-dependent expression of mouse Gata3

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Gata3 (Gata3 Mouse)

Use

Lentiviral

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Eomes

Plasmid

#128825

Purpose

dox-dependent expression of mouse Eomes

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Eomes (Eomes Mouse)

Use

Lentiviral

Mutation

Shorter isoform

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-K525E

Plasmid

#91985

Purpose

Expresses FLAG-HA-AGO2 (K525E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

K525E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P2-MDMX (1-120)

Plasmid

#62065

Purpose

Expression of human MDM2 (1-120) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

MDM4 (MDM4 Human)

Tags

GST

Expression

Bacterial

Mutation

contains residues 1-120

Promoter

tac

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-BEACON1

Plasmid

#171697

Purpose

Expresses BEACON1 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

Insert

BEACON1

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_W104A/Y132D

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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px330-CIB1-dCas9

Plasmid

#63665

Purpose

Mammalian gRNA expression vector also expressing CIB1-dCas9

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 and CMV

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-WT

Plasmid

#92009

Purpose

Expresses Lambda-N-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

Lambda-N-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgNCKAP1-2

Plasmid

#210134

Purpose

knock out NCKAP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Nck-associated protein 1 (NCKAP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgNCKAP1-1

Plasmid

#210133

Purpose

knock out NCKAP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Nck-associated protein 1 (NCKAP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTMB340_ZFcharm_Kv1_Prnp_DPM

Plasmid

#220843

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-FL

Plasmid

#202515

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ265G2

Plasmid

#164723

Purpose

Gateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A(Y130F)-ziSpyMac(D10A)-UGI)

Use

CRISPR; Gateway compatible a3a/y130f-ispymaccas9-…

Expression

Plant

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-UCHL5

Plasmid

#31219

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

UCHL5 (UCHL5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 19, 2011

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-NDC80

Plasmid

#31215

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NDC80 (NDC80 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-SM

Plasmid

#136578

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-E2A-cMyc (Sox2, Myc Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-D358K

Plasmid

#91983

Purpose

Expresses FLAG-HA-AGO2 (D358K)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D358K

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.27_eSOX17

Plasmid

#195498

Purpose

Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the extended endoderm distal enhancer of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Full distal endoderm enhancer region of SOX17 (SOX17 Human)

Tags

minP_luc2-hPEST

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.27_eSOX17.2

Plasmid

#195499

Purpose

Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Core distal endoderm enhancer region of SOX17 (SOX17 Human)

Tags

minP_luc2-hPEST

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-90) (72P)

Plasmid

#62080

Purpose

Expression of human p53 (1-90) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD/PP residues 1-90; 72P

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
T7pr-His6-MBP-TEV-dCas9-BirA*

Plasmid

#159994

Purpose

Bacterial expression of dCas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

dCas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAdEasy-hALDOA

Plasmid

#210812

Purpose

To package adenovirus expressing wild-type aldolaseA

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Adenoviral

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCOQ2

Plasmid

#186025

Purpose

knock out COQ2 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Coenzyme Q2, Polyprenyltransferase (COQ2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-SK

Plasmid

#136577

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-T2A-Klf4 (Klf4, Sox2 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ269C2

Plasmid

#164716

Purpose

Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

hAID-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible haid-cbe_v04 entry clo…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)

Plasmid

#209775

Purpose

CRISPRi for STK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-Sniper-KRAB, sgSTK3i (STK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-mouse Gfi1-IRES-GFP

Plasmid

#91893

Purpose

Mammalian expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pMXs-hSOX2

Plasmid

#193351

Purpose

Retroviral vector expressing human SOX2

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-WT

Plasmid

#91986

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge