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We narrowed to 18,759 results for: nls

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Showing: 5521 - 5560 of 18759 results

  11. pDN-D2irTN6kwh

    Plasmid
    #44724
    Depositor
    Gabor Balazsi
    Publication
    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pCMV-D2i promoter
    htetR::NLS
    Use
    Synthetic Biology; Expression regulator/reporter;…
    Tags
    Rabbit β-globin intron II and WPRE
    Expression
    Mammalian
    Mutation
    Initiator motif (Inr) displaced relative to pCMV-…
    Promoter
    pCMV-D2i
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    44724-plasmid-map-sequence-id-61266

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  16. pGAL1-VP1 (LEU2)

    Plasmid
    #182480
    Purpose
    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al bioRxiv 2022.11.24.517869
    Insert
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182480-plasmid-map-sequence-id-366129

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  18. SpCas9-NRCH-SsAPOBEC3B

    Plasmid
    #198550
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-SsAPOBEC3B
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198550-plasmid-map-sequence-id-388894

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  19. SpCas9-NG-CGBE1

    Plasmid
    #198537
    Purpose
    Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-CGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198537-plasmid-map-sequence-id-388909

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  20. MTK0_015

    Plasmid
    #123930
    Purpose
    Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB Destination - Insulator + tagBFP
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123930-plasmid-map-sequence-id-250082

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  21. pHS1518

    Plasmid
    #205981
    Purpose
    FLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas6(Cse3/CasE) from MWEE01000013, Cas5-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    205981-plasmid-map-sequence-id-424945

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  22. pHS1514

    Plasmid
    #205977
    Purpose
    FLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas8/Cse1 from MWEE01000013, Cas5-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205977-plasmid-map-sequence-id-416243

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  23. pHS1515

    Plasmid
    #205978
    Purpose
    FLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas11/Cse2 from MWEE01000013, Cas5-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205978-plasmid-map-sequence-id-415384

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  24. pHS1516

    Plasmid
    #205979
    Purpose
    FLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas7 from MWEE01000013, Cas5-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205979-plasmid-map-sequence-id-416228

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  26. pPyCAG-NE-EGFP-IRES-Pac

    Plasmid
    #183617
    Purpose
    Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.
    Depositor
    Sally Lowell
    Publication
    Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Tags
    Human EMD transmembrane domain and NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183617-plasmid-map-sequence-id-361613

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  29. VRER variant

    Plasmid
    #138561
    Purpose
    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRER
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138561-plasmid-map-sequence-id-274783

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  30. VRQR variant

    Plasmid
    #138562
    Purpose
    Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138562-plasmid-map-sequence-id-274786

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  31. frq[ΔNLS, mNG_V5]_nat-1

    Plasmid
    #251399
    Purpose
    Encodes an NLS1-deleted frq allele with C-terminal mNeonGreen and V5 tags, enabling fluorescent visualization and detection following replacement of the endogenous frq locus.
    Depositor
    Jay C Dunlap
    Publication
    Wang et al Genetics. 2026 May 18:iyag126. doi: 10.1
    Insert
    frq
    Use
    Neurospora expression
    Tags
    V5 and mNeonGreen
    Mutation
    deleted amino acids 95-100
    Available since
    May 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    251399-plasmid-map-sequence-id-499116

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  32. pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-L22Q/W23S/W53Q/F54S-lin-hCRY2

    Plasmid
    #241847
    Purpose
    Expresses a negative control of the actuator of Opto-p53 in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Publication
    Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.
    Insert
    p53 (TP53 Human)
    Tags
    CRY2 and miRFP703
    Expression
    Mammalian
    Mutation
    N-terminus (1-97 aa) containing L22Q/W23S/W53Q/F5…
    Promoter
    CAGGS promoter
    Available since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    241847-plasmid-map-sequence-id-485118

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  33. pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210743
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 3
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210743-plasmid-map-sequence-id-420083

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  34. pUC57-ITR2-H1-GFP sgRNA Merry Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210744
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 4
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 4
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210744-plasmid-map-sequence-id-420033

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  35. pUC57-ITR2-H1-GFP sgRNA Gollum Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210747
    Purpose
    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 3
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Gollum sgRNA GFP Target 3
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210747-plasmid-map-sequence-id-418044

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  36. pUC57-ITR2-H1-GFP sgRNA Gollum Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210748
    Purpose
    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 4
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Gollum sgRNA GFP Target 4
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210748-plasmid-map-sequence-id-417999

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  37. pUC57-ITR2-H1-GFP sgRNA Gollum Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210746
    Purpose
    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 2
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Gollum sgRNA GFP Target 2
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210746-plasmid-map-sequence-id-417992

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  38. pUC57-ITR2-H1-GFP sgRNA Merry Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210741
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 1
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 1
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210741-plasmid-map-sequence-id-417990

    Enlarge

  39. pUC57-ITR2-H1-GFP sgRNA Gollum Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210745
    Purpose
    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 1
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Gollum sgRNA GFP Target 1
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210745-plasmid-map-sequence-id-417963

    Enlarge

  40. pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210742
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 2
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210742-plasmid-map-sequence-id-417959

    Enlarge

Showing: 5521 - 5560 of 18759 results