We narrowed to 8,864 results for: BLI;
-
Plasmid#216573Purposegateway cloning donor vector containing DDX3X ASD mutationDepositorInsertDDX3X (DDX3X Human)
UseGateway entry plasmidTagsExpressionMutationI107VPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
SOX2-P2A-NLS-mClover3-IRES-Puro
Plasmid#215544PurposeDonor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locusDepositorInsertSOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApaLIE225A, K227A-HA
Plasmid#211460PurposeExpress mitochondrial localized ApaLI with E225A and K227A mutationsDepositorInsertApaLI-E225A-K227A
UseTagsHAExpressionMammalianMutationE225A, K227APromoterCMVAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep
Plasmid#208644Purposefor long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
UseCRISPR; Ssdna donnor preparationTagsTwin StrepExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-msEGFP1.8
Plasmid#208619PurposeMammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.DepositorInsertmsEGFP1.8
UseTagsExpressionMammalianMutation(compared with pEGFP-C1, protein level) S30R, Y39…PromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
GOSR2(G144W)-mCherry
Plasmid#209887PurposeExpresses human GOSR2 (GS27) G144W-mutant fused to mChery in mammalian cellsDepositorInsertGOSR2 (GOSR2 Human)
UseTagsmCherryExpressionMammalianMutationChanged glycine 144 to tryptophan; this mutation …PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_69.11
Plasmid#194214PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI
Plasmid#192113PurposeExpresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-BA.4/5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
human KANK2-A670V Ankyrin Repeats pET151
Plasmid#164055PurposeExpresses human KANK2 Ank domain - A670V mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK3 Ankyrin Repeats pET151
Plasmid#164056PurposeExpresses mouse KANK3 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 3 (Kank3 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4 Ankyrin Repeats pET151
Plasmid#164057PurposeExpresses mouse KANK4 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
loxP-T2A-Myr-DeadCasp8-ERT2-rox-FNF-loxP-T2A-Myr-Casp8-ERT2-rox
Plasmid#182360PurposeShuttle vector for T2A-Myr-DeadCasp8-ERT2 and T2A-Myr-Casp8-ERT2DepositorInsertMyr-DeadCasp8-ERT2 and Myr-Casp8-ERT2
UseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDZ2FE_Champ1_1-812_2PA
Plasmid#105676PurposeExpresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 bindingDepositorInsertChamp1 (CHAMP1 Human)
UseTags2X Flag tagExpressionMammalianMutationMutations: P336A, P341APromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT5T_Rev7_1-211_SA112_KVK_YAK
Plasmid#105630PurposeExpresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3DepositorInsertRev7 (Mad2l2 Mouse)
UseTagsExpressionBacterialMutationMutations: F11S, G12A, I41Y, V132K, C133V, A135K,…PromoterT7Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_B.1.640
Plasmid#180228PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the B.1.640 SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationB.1.640 (Amino Acid 319-869)PromoterT7Available sinceJan. 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
CD44U-c010
Plasmid#175453PurposeProtein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649.DepositorInsertCD44 (CD44 Human)
UseTagsHis6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_C.1.2
Plasmid#177367PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the C1.2 SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationC1.2 (Amino Acid 319-869)PromoterT7Available sinceJan. 20, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Mu
Plasmid#177366PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Mu SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationMu (Amino Acid 319-869)PromoterT7Available sinceJan. 20, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Kappa
Plasmid#175953PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Kappa SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationKappa (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Eta
Plasmid#175954PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Eta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationEta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Gamma
Plasmid#175047PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Gamma SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationGamma (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Theta
Plasmid#175951PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Theta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationTheta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Zeta
Plasmid#175952PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Zeta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationZeta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pChd4EosHaloTAF_Puro
Plasmid#172099PurposeTargeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusionDepositorInsertchromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)
UseMouse TargetingTags3xFLAG, Avi, Eos, Halo, and TEVExpressionMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)
Plasmid#174139PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationVRQR=D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons
Plasmid#172480PurposeH2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin geneDepositorInsertH2B-sfGFP-T2A-tdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB6-Id1-T2A-tdTomato-FNF-TK
Plasmid#139281PurposeB6J Id1-T2A-tdTomato allele targeting vector, low copy numberDepositorInsertId1 (Id1 Mouse)
UseMouse TargetingTagsC-terminal fusion of T2A-tdTomatoExpressionMammalianMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB6-Gfap-T2A-td-sfGFP-FNF-DTA
Plasmid#137044PurposeB6J Gfap-T2A-td-sfGFP allele targeting vector, low copy numberDepositorInsertGfap (Gfap Mouse)
UseMouse TargetingTagsT2A-td-sfGFPExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFE-sfGFP
Plasmid#132998PurposepFE carrying a sfGFP geneDepositorInsertsfGFP
UseTags6xhisExpressionBacterialMutationPromotertetRAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabA2
Plasmid#133001PurposepFE carrying a dabA2 gene (Uniprot: D0KWS7)DepositorInsertdabA2
UseTagsExpressionBacterialMutationPromotertetRAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabB2
Plasmid#133000PurposepFE carrying a dabB2 gene (Uniprot: D0KWS8)DepositorInsertdabA2
UseTagsExpressionBacterialMutationPromotertetRAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ad-mMKP3 S159A S197A-Flag
Plasmid#131579PurposeExpresses MKP-3 with S159A mutation and S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsFlagExpressionMutationchanged Serine 159 to Alanine and Serine 197 to A…PromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse 3' Ubf AID GFP PuroR
Plasmid#127901PurposeHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf GeneDepositorInsertUbf (Ubtf Mouse, Mustard Weed)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationInserting GFP AID 2A Puro into mouse 3' UbfPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI119
Plasmid#126059PurposeModule 0 vector of PlaMiNGo toolkitDepositorInsertchloroplastic FNR transit peptide
UseSynthetic Biology; Module 0TagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI120
Plasmid#126060PurposeModule 0 vector of PlaMiNGo toolkitDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseSynthetic Biology; Module 0TagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGRNA-LbCpf1-SpCas9
Plasmid#119673PurposeEmpty vector for cloning LbCpf1-SpCas9 fgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGRNA-AsCpf1-SpCas9
Plasmid#119674PurposeEmpty vector for cloning AsCpf1-SpCas9 fgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGRNA-LbCpf1-spCas9-MS2
Plasmid#119675PurposeEmpty vector for cloning LbCpf1-spCas9 MS2-fgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1 (in pCAGGS)
Plasmid#118039PurposeGABAA receptor expression (gamma1 subunit) Myc-tag near N-terminusDepositorInsertGABRG1 (Gabrg1 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.zeo/hKGA.S286A
Plasmid#110357PurposeMammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tagDepositorInsertKGA glutaminase (GLS Human)
UseRetroviralTagsV5ExpressionMammalianMutationS286APromotergagAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6-Halo-TEV-Akt2
Plasmid#87606Purposeexpresses His-halo tagged at the N-terminus of human Akt2 proteinDepositorInsertAKT 2 (AKT2 Human)
UseZebrafish (danio rerio) expressionTagsHis-Halo-TevExpressionMammalianMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b M72C
Plasmid#83160PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), M72C mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationM72CPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b R73K
Plasmid#83163PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R73K mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationR73KPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b R73E
Plasmid#83162PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R73E mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationR73EPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b Y40F
Plasmid#83161PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Y40F mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationY40FPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b Y40C
Plasmid#83159PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Y40C mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationY40CPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b E37G
Plasmid#83158PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), E37G mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationE37GPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b T35S
Plasmid#83157PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), T35S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationT35SPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b K147Q
Plasmid#83155PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K147Q mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationK147QPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only