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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,659 results for: SUB;

Showing: 6901 - 6950 of 9659 results

  1. pOpen-BsupolLF

    Plasmid
    #165563
    Purpose
    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Bsu DNA Polymerase I, Large Fragment
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165563-plasmid-map-sequence-id-340036

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  4. NR4A1

    Plasmid
    #25477
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR4A1 (NR4A1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pAAV-IV-3xFLAG/HA/NBL10

    Plasmid
    #110568
    Purpose
    Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag
    Depositor
    Alexander Nectow, Jeffrey Friedman
    Insert
    3xFLAG/HA-tagged anti-GFP VHH
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    110568-plasmid-map-sequence-id-213334

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  13. NR1D2

    Plasmid
    #25186
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR1D2_07 (NR1D2 Human)
    Use
    Baculovirus expression
    Tags
    His
    Promoter
    polyhedrin
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. p203 Dync1li1 N235Y

    Plasmid
    #26451
    Depositor
    Elizabeth Fisher
    Insert
    cytoplasmic dynein light intermediate chain 1 N235Y (Dync1li1 Mouse)
    Use
    Subcloning and sequencing
    Mutation
    changed Asparagine 235 to Tyrosine
    Available Since
    Dec. 2, 2010
    Availability
    Academic Institutions and Nonprofits only

  16. CavB1/pFLAG-myc-CMV

    Plasmid
    #197301
    Purpose
    Plasmid expressing an antigen targeting the Cavbeta1 calcium channel subunit
    Depositor
    Ricardo Dolmetsch
    Insert
    Cavb1 (CACNB1 Human)
    Tags
    Myc, FLAG
    Expression
    Mammalian
    Available Since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    197301-plasmid-map-sequence-id-407093

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  17. Slack Slo2.2 max1-ECFP

    Plasmid
    #197318
    Purpose
    Plasmid expressing an antigen used to generate an antibody that targets the Slack K+ channel subunit
    Depositor
    Christopher Lingle
    Insert
    KCNT1/Slo2.2/Slack K+ channel (Kcnt1 Mouse)
    Tags
    ECFP
    Expression
    Mammalian
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197318-plasmid-map-sequence-id-388160

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  18. pVP16-tev-PRT1-C195A

    Plasmid
    #83408
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C195A substitution
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83408-plasmid-map-sequence-id-175084

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  19. pVP16-tev-PRT1-C29A

    Plasmid
    #83407
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C29A substitution
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83407-plasmid-map-sequence-id-175083

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  20. pVP16-tev-PRT1-V28A

    Plasmid
    #83406
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    V28A substitution
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83406-plasmid-map-sequence-id-175082

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  21. pDRGFP

    Plasmid
    #26475
    Purpose
    In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker
    Depositor
    Maria Jasin
    Article
    Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.
    Insert
    DRGFP
    Expression
    Mammalian
    Available Since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26475-plasmid-map-sequence-id-432928

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  22. TRMT10C (RG9MTD1A-c113)

    Plasmid
    #110248
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RG9MTD1 (TRMT10C Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110248-plasmid-map-sequence-id-212286

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  24. pSEVA3b67Rb

    Plasmid
    #58311
    Purpose
    Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Insert
    mRFP1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58311-plasmid-map-sequence-id-89824

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Showing: 6901 - 6950 of 9659 results