We narrowed to 4,490 results for: GCA;

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VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck

Plasmid

#58524

Purpose

Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S, E154A)-TS-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine; changed Glutamate…

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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QUAS-jGCaMP7s-T2A-tdTomato

Plasmid

#200010

Purpose

QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

Depositor

Carolyn McBride

Article

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

3xP3-ECFP-jGCaMP7s-td-Tomato

Expression

Insect

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RiboL1-jGCaMP8f

Plasmid

#192612

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RiboL1-jGCaMP8m

Plasmid

#192613

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx46-IRES-GCaMP6s

Plasmid

#188238

Purpose

3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6s

Depositor

Fabio Mammano

Article

Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

Insert

GJA3 (GJA3 Human)

Use

Lentiviral

Tags

GCaMp6s

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx26-IRES-GCaMP6s

Plasmid

#188236

Purpose

3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 and GCaMP6s

Depositor

Fabio Mammano

Article

Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

Insert

GJB2 (GJB2 Human)

Use

Lentiviral

Tags

GCaMP6s

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE-dTomato-GCaMP6s

Plasmid

#99864

Purpose

expresses dTomato and GCaMP6s under control of the Tet-off system

Depositor

Venkatesh Murthy

Article

Wallace et al Elife. 2020 Mar 9;9. pii: 50531. doi: 10

Insert

dTomato, GCaMP6s

Promoter

Tet-responsive promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-synp-F-H2B-GCaMP6f

Plasmid

#102994

Purpose

Expresses histone fused GCaMP6f under the synapsin promoter

Depositor

David Cox

Article

Skocek et al Nat Methods. 2018 Jun;15(6):429-432. doi

Insert

H2B-GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

human synapsin I promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-GCaMP6f

Plasmid

#249680

Purpose

Expresses GCaMP6f under the CAG promoter

Depositor

Takeshi Imai

Article

Inagaki et al Nat Methods. 2026 Mar 12. doi: 10.1038/s

Insert

GCaMP6f

Expression

Mammalian

Promoter

CAG

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgCASP8

Plasmid

#211519

Purpose

Deletes CASP8

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgCASP8

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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vgCam-pRSETb

Plasmid

#193362

Purpose

A FRET-based calcium probe using Sumire

Depositor

Takeharu Nagai

Article

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Insert

vgCam

Use

E.coli expression

Tags

His X6 tag and Strep tag

Promoter

T7

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.NgCAM-Halo

Plasmid

#162719

Purpose

Mammalian expression of chicken NgCam

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

NgCam (L1CAM Chicken)

Tags

Halo

Expression

Mammalian

Mutation

E137K, K138E, and K642E

Promoter

Chicken Beta actin

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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spa-GCaMP6m

Plasmid

#67557

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6m

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
AAV Cag Flex H2B Gcamp6s reverse

Plasmid

#74157

Purpose

Calcium Sensor

Depositor

Loren Looger

Insert

Gcamp6s

Use

AAV

Tags

H2B

Promoter

SpeI

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s

Plasmid

#200400

Purpose

Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider ant

Depositor

Daniel Kronauer

Article

Hart et al Cell. 2023 Jul 6;186(14):3079-3094.e17.

Inserts

Discosoma red fluorescent protein
Q factor 2
GCaMP6s

Expression

Insect

Promoter

15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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Orai1-jGCaMP8f V203Y

Plasmid

#231631

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

Insert

Orai1-jGCaMP8f V203Y (ORAI1 Human, Synthetic)

Tags

jGCaMP8f V203Y

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f F366H

Plasmid

#204139

Purpose

Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Phenylalanine 366 changed to histidine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-jGCaMP8f

Plasmid

#172387

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

GCaMP8f

Use

Neurotropic virus

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-ProA18-GCaMP6s

Plasmid

#126004

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GCaMP6s

Use

AAV

Promoter

ProA18

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-RS14

Plasmid

#52651

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS14

Expression

Mammalian

Mutation

deleted G47 from GCaMP3

Promoter

T7

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
AAV-syn-axon-jGCaMP8s-WPRE

Plasmid

#186043

Purpose

jGCaMP8s expression in axons under the control of the synapsin promoter

Depositor

Loren Looger

Insert

axon-jGCaMP8s

Use

AAV

Tags

GAP43-6xHis

Expression

Mammalian

Promoter

synapsin

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

Plasmid

#117384

Purpose

An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 E484A (GCA)

Plasmid

#212471

Purpose

Encodes SARS-CoV-2 spike E484A for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484A (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484A

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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AAV Synapsin Intron H2B Gcamp 6f wpre Pzac2.1

Plasmid

#74144

Purpose

Calcium sensor

Depositor

Loren Looger

Insert

GCaMP6f

Use

AAV

Tags

H2B

Promoter

synapsin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Axon-jGCaMP8s

Plasmid

#239672

Purpose

Cre-dependent expression of axon targeted jGCaMP8s under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8s

Use

AAV

Tags

GAP43Pal

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-DIO-GCaMP6s

Plasmid

#184284

Purpose

Expresses GCaMP6s in genetically defined neurons expressing Cre recombinase

Depositor

William Wisden

Article

Yu et al Nat Neurosci. 2019 Jan;22(1):106-119. do

Insert

CCaMP6s

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP

Expression

Mammalian

Promoter

human synaptophysin

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.mCherry-GCaMP7s

Plasmid

#172385

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized mCherry + GCaMP7s

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pKL12-GCaMP6f-pHuji bb118

Plasmid

#158981

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-pHuji

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833 a…

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugCas9-NNG-P2A-puro

Plasmid

#214352

Purpose

Expresses SlugCas9-NNG and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9-NNG and puromycin resistance genes

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-syn-axon-jGCaMP8m-WPRE

Plasmid

#186042

Purpose

jGCaMP8m expression in axons under the control of the synapsin promoter

Depositor

Loren Looger

Insert

axon-jGCaMP8m

Use

AAV

Tags

GAP43-6xHis

Expression

Mammalian

Promoter

synapsin

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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