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Plasmid#217535PurposeAAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivoDepositorArticleInsert4X BRE reporter and miniXon casette (Smad1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterBMP response element (BRE)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -2
Plasmid#173891PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 Gatad2a sgRNA (for flox targeting)
Plasmid#110815PurposeUsed with pNTK Gatad2a flox allele targeting construct in mouse cellsDepositorInsertmGataD2a (Gatad2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW759_pCAG-PV2-lox2272-GFP-BGHpA
Plasmid#87575PurposePV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full AdderDepositorInsertEGFP
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW807_pCAG-PV4-loxP-mCherry-BGHpA
Plasmid#87583PurposePV4 for 3-input, 2-output Full AdderDepositorInsertmCherry
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW770_pCAG-PV4-loxP-mRuby2-BGHpA
Plasmid#87577PurposePV4 for 2-input, 4-output decoder circuitDepositorInsertmRuby2
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW707_pCAG-PV3-FRT-loxP-mCherry
Plasmid#87571PurposePV3 for 2-input, 2-output circuit libraryDepositorInsertmCherry
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW705_pCAG-PV1-FRT-lox2272-STOP
Plasmid#87569PurposePV1 for 2-input, 2-output circuit libraryDepositorInsertNeomycin Resistance Gene
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-IRES-Ofd1-Myc-S75F
Plasmid#24574DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationserine 75 changed to phenylalanine (codon TCT cha…PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-IRES-Ofd1-Myc-A80T
Plasmid#24575DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationAlanine 80 changed to threonine (codon GCA to ACA)PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-IRES-Ofd1-Myc-G139S
Plasmid#24576DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationGlycine 139 changed to serine (codon GGT to AGT)PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-Ofd1-Myc-KDD359-361FSY
Plasmid#24578DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationLysine-Aspartate-Aspartate 359-361 changed to Phe…PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNTK human MBD3 PGK-Neo-pA flox
Plasmid#52362PurposeTargeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.DepositorInsertMBD3 (MBD3 Human)
UseTargetingTagsExpressionMutationPromoterAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-loxp Kir2.1-2A-GFP
Plasmid#161574PurposeExpression of Kir2.1-2A-GFP in a Cre-dependent fashionDepositorInsertKir2.1-2A-GFP (Kcnj2 Mouse)
UseAAVTagsExpressionMutationNAPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAHR TH-p2a-TD:Tomato (floxed selection Puro)
Plasmid#135814PurposeFluorescent reporter for knock-in of TdTomato into the TH locusDepositorInsertTH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
UseTagsp2a-TdTomatoExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-double floxed-eNpHR-EYFP-WPRE-pA
Plasmid#20949PurposeCre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibitionDepositorHas ServiceAAV9Inserthalorhodopsin
UseAAVTagsEYFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-loxp hM4Di-mCherry
Plasmid#161581PurposeExpression of hM4Di-mCherry in a cre-dependent fashionDepositorInserthM4Di-mCherry
UseAAVTagsExpressionMutationNAPromoterhSynAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Triple ORF LoxP ifgMosaic Donor (LH208)
Plasmid#99625PurposeTo clone the 3 ORFs for Cre dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/Lox, Mouse Targeting, and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTubLinker-TYx3-mNeon-TYx3-HX(floxed).dna
Plasmid#166237PurposeC-terminal Ty-mNeon, floxed HXGPRT markerDepositorInsertTy tag fused to mNeon
UseTagsExpressionMutationnonePromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW-lox-mCherry-hyBRET-ERK-NLS
Plasmid#170290PurposeFor generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.DepositorInsertlox-mCherry-lox-hyBRET-ERK-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-GST-Nter-GWs-Lox (VE5586)
Plasmid#163766PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.DepositorInsertN-terminal GST tag
UseTagsGST TagExpressionInsectMutationPromoterPHAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-6His-Nter-GWs-Lox (VE5587)
Plasmid#163768PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter.DepositorInsertN-terminal 6His tag
UseTags6 His TagExpressionInsectMutationPromoterPHAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-10His-Nter-GWs-Lox (VE5588)
Plasmid#161806PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.DepositorInsertN-terminal 10His tag
UseTags10 HisExpressionInsectMutationPromoterPHAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpA
Plasmid#87586PurposePV7 for 3-input, 2-output Full AdderDepositorInsertmCherry
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-sfGFP-tdTomato T2A V XE
Plasmid#113853PurposeSuperfolder GFP and tdTomato linked by T2A (V linker)DepositorInsertsfGFP-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW708_pCAG-PV4-loxP-GFP-T2A-mCherry
Plasmid#89057PurposePV4 for 2-input, 2-output circuit libraryDepositorInsertGFP-T2A-mCherry
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA
Plasmid#87574PurposePV1 for 2-input, 4-output decoder circuitDepositorInsertmtagBFP
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
loxP-T2A-Myr-Casp8-ERT2-rox
Plasmid#182362PurposeShuttle vector for T2A-Myr-Casp8-ERT2DepositorInsertloxP-T2A-Myr-Casp8-ERT2-rox
UseCre/Lox and Mouse Targeting; Dre / roxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
loxP-T2A-Myr-DeadCasp8-ERT2-rox
Plasmid#182361PurposeShuttle vector for T2A-Myr-DeadCasp8-ERT2DepositorInsertloxP-T2A-Myr-DeadCasp8-ERT2-rox
UseCre/Lox and Mouse Targeting; Dre / roxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E
Plasmid#125797PurposeHistone H2B-superfolder GFP and tdTomato fused by unskippable linkerDepositorInsertH2BsfGFP-tdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only