We narrowed to 2,687 results for: fas

1. pFastBac-M13-6His-KIF1C-GFP

   Plasmid

   #130975

   Purpose

   Expression of 6His-KIF1C-GFP in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Use

   Tags

   6His and GFP

   Expression

   Insect

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   Polyhedrin

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b-His-TEV-CLIP-tag_fast

   Plasmid

   #167275

   Purpose

   Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   CLIPf-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   E30R

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac-His-MBP-SCov2-nsp12

   Plasmid

   #154759

   Purpose

   Expression of SARS-CoV-2 nsp12

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedron

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac Dual LIC cloning vector (5A)

   Plasmid

   #30121

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pan-Neurofascin (extracellular) scFv [A12/18]

   Plasmid

   #190485

   Purpose

   Mammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-FAS-EGFP-WPRE-pA

   Plasmid

   #37091

   Purpose

   Expresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   d1eGFP
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFAST-BAC BAF57/SMARCE1-His

   Plasmid

   #177861

   Purpose

   Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

   Depositor

   Yoshihiro Izumiya

   Article

   Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

   Insert

   SMARCE1 (SMARCE1 Human)
   

   Use

   Tags

   His tag at C-terminus with GGGGS linker

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn-FAS-DO-ReaChR-mCitrine-WPRE

   Plasmid

   #64035

   Purpose

   Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs

   Depositor

   Karel Svoboda

   Insert

   hSyn-FAS-DO-ReaChR-mCitrine-WPRE
   

   Use

   AAV

   Tags

   ReaChR tagged with mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pIG-835_NY-ESO-1_TCR_FAS/MyD88

   Plasmid

   #207498

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   FAS/MyD88, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET51b-His-TEV-SNAP-tag_fast

    Plasmid

    #167271

    Purpose

    Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac-Dual- GST-TEV-TBK1

    Plasmid

    #208875

    Purpose

    For expression in insect cells of TBK1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid

    #216377

    Purpose

    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

    Depositor

    William Weis

    Insert

    LDL receptor related protein 6 (LRP6 Human)
    

    Use

    Baculovirus

    Tags

    9xHis and HA signal sequence-FLAG-3C cleavage sit…

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin (LRP6)/p10 (MESD)

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIG-832_NY-ESO-1_TCR_FAS/4-1BB

    Plasmid

    #207495

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/4-1BB, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIG-834_NY-ESO-1_TCR_FAS/IL4R

    Plasmid

    #207497

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/IL4R, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid

    #64064

    Purpose

    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

    Depositor

    Andrew Carter

    Article

    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

    Insert

    Cytoplasmic dynein-2 (DYNC2H1 Human)
    

    Use

    Tags

    GFP, His tag, and ZZ tag

    Expression

    Insect

    Mutation

    Deleted amino acids 1-1090, extra C-terminal vali…

    Promoter

    Polyhedrin

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac (GST-preSci site-CD45)

    Plasmid

    #177889

    Purpose

    Baculo expression of GST tag fused to human CD45 ICD

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    CD45 (aa 598-1304 only) (PTPRC Human)
    

    Use

    Tags

    TEV-GST-PreScission cut site

    Expression

    Insect

    Mutation

    L652P

    Promoter

    Polyhedrin

    Available since

    Aug. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIG-836_NY-ESO-1_TCR_FAS/OX40

    Plasmid

    #207499

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/OX40, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIG-833_NY-ESO-1_TCR_FAS/CD28

    Plasmid

    #207496

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/CD28, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWZL Neo Myr Flag FASTK

    Plasmid

    #20485

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    FASTK (FASTK Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac1-OSF-TRIM5alpha(human)(R332P)

    Plasmid

    #79037

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(human R332P) (TRIM5 Human)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Changed Arg 332 to Pro

    Promoter

    polyhedrin

    Available since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGL3-Basic-Fascin-Promoter (-402-0)

    Plasmid

    #89823

    Purpose

    Promoter luciferase assay

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Fascin1 (FSCN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFastBac-His-Flag-Tipin-L195A

    Plasmid

    #23120

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 Mar 15. ():.

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    Flag- and His

    Expression

    Insect

    Mutation

    Changed Leucine 195 to Alanine

    Promoter

    Available since

    March 3, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pFastBac-M13-8xHis-ZZ-LTLT-HOOK3

    Plasmid

    #222287

    Purpose

    Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease).

    Depositor

    Anne Straube

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Insert

    HOOK3 (HOOK3 Human)
    

    Use

    Tags

    8 His and ZZ tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ER-mit RspA-splitFAST (lentiviral plasmid)

    Plasmid

    #233604

    Purpose

    Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-splitFAST
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV/SV40

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST

    Plasmid

    #223764

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFastBac HTB His-TEV-rDPP9

    Plasmid

    #217326

    Purpose

    Purification of rat DPP9 from insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid

    #141237

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    Human elongation factor-1 alpha (EF-1 alpha)

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

    Plasmid

    #216378

    Purpose

    Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    HA signal sequence-FLAG

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

    Plasmid

    #198037

    Purpose

    Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST-TEV-EGFP

    Expression

    Insect

    Mutation

    E696K

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFastBac-GST-TEV-mCherry-TBK1

    Plasmid

    #198033

    Purpose

    Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST-TEV and mCherry

    Expression

    Insect

    Mutation

    E696K

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge