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We narrowed to 573 results for: TRE3G

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Showing: 521 - 560 of 573 results

  1. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid
    #89463
    Purpose
    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)
    Depositor
    Brad Zuchero
    Article
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Tags
    E. coli DHFRdd destabilization domain and mCherry…
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    TRE3G
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    89463-plasmid-map-sequence-id-176808

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  5. pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR

    Plasmid
    #204151
    Purpose
    Lentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes
    Depositor
    Jesse Bloom
    Article
    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.
    Insert
    SARS-CoV-2 Delta variant spike
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    21 amino acid cytoplasmic tail deletion
    Promoter
    TRE3G
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    204151-plasmid-map-sequence-id-404782

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  9. pTetOne-human cGASdeltaN-HA

    Plasmid
    #186897
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186897-plasmid-map-sequence-id-366541

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  10. pTetOne-orangutan cGASdeltaN-HA

    Plasmid
    #186898
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Synthetic, Pongo abelii)
    Use
    Retroviral
    Tags
    HA
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186898-plasmid-map-sequence-id-366562

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  15. pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

    Plasmid
    #204146
    Purpose
    Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes
    Depositor
    Jesse Bloom
    Article
    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.
    Inserts
    SARS-CoV-2 BA.1 variant spike
    zsGreen-T2A-PuR
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    21 amino acid cytoplasmic tail deletion
    Promoter
    CMV and TRE3G
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    204146-plasmid-map-sequence-id-404779

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  16. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid
    #155189
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP
    Depositor
    Xiaoping Bao
    Article
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Insert
    beta-catenin (Ctnnb1 Mouse)
    Use
    Unspecified; Piggybac transposon
    Tags
    P2A eGFP NLS
    Expression
    Mammalian
    Mutation
    Sites phosphorylated by GSK-3beta have been mutat…
    Promoter
    TRE3G
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155189-plasmid-map-sequence-id-307963

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  21. pTetOne-FLAG-human cGASdeltaN-HA

    Plasmid
    #186899
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    FLAG and HA
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186899-plasmid-map-sequence-id-366534

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  22. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available Since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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  24. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-490369

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  28. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Article
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available Since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  29. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  30. pGFP_1

    Plasmid
    #238192
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP and SV40
    Expression
    Mammalian
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238192-plasmid-map-sequence-id-472672

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  31. pSNAP_1

    Plasmid
    #238196
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to SNAP-tag, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    SNAPtag and SV40
    Expression
    Mammalian
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238196-plasmid-map-sequence-id-472671

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  32. pSNAP_24

    Plasmid
    #238199
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to SNAPtag-FTH1(24-mer), under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    SNAPtag-FTH1(24mer) and SV40
    Expression
    Mammalian
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238199-plasmid-map-sequence-id-472676

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  33. pSNAP_6

    Plasmid
    #238198
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to SNAPtag-6mer_oligomerization_domain, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    SNAPtag-6mer and SV40
    Expression
    Mammalian
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238198-plasmid-map-sequence-id-472674

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  34. pGFP_24

    Plasmid
    #238195
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP-FTH1(24-mer), under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP-FTH1(24mer) and SV40
    Expression
    Mammalian
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238195-plasmid-map-sequence-id-472670

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  35. pSNAP_4

    Plasmid
    #238197
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to SNAPtag-4mer_oligomerization_domain, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    SNAPtag-4mer and SV40
    Expression
    Mammalian
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238197-plasmid-map-sequence-id-472669

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  36. pGFP_4

    Plasmid
    #238193
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP-4mer_oligomerization_domain, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP-4mer and SV40
    Expression
    Mammalian
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238193-plasmid-map-sequence-id-472663

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  37. pGFP_6

    Plasmid
    #238194
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP-6mer_oligomerization_domain, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP-6mer and SV40
    Expression
    Mammalian
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238194-plasmid-map-sequence-id-472655

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  38. pLV-FLAG-NF2-WT

    Plasmid
    #253101
    Purpose
    lentiviral FLAG-tagged NF2, dox-inducible
    Depositor
    Harish Vasudevan
    Insert
    FLAG-tagged NF2 (NF2 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    253101-plasmid-map-sequence-id-504888

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Showing: 521 - 560 of 573 results