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Showing: 651 - 700 of 790 results

  1. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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  3. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125199-plasmid-map-sequence-id-243587

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  4. pSBbi-Pur-AktAR2-D

    Plasmid
    #125196
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2-D
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125196-plasmid-map-sequence-id-243601

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  7. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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  22. pSBbi-Pur-Lyn-AktAR2-EV-D

    Plasmid
    #125200
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV-D
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125200-plasmid-map-sequence-id-243591

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  43. pRSETA_crE6G2

    Plasmid
    #172929
    Purpose
    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain
    Depositor
    Arnold Boersma
    Article
    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.
    Insert
    CrowdingSensorE6G2
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Val317Gly, Met316Gly
    Promoter
    T7
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172929-plasmid-map-sequence-id-339474

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Showing: 651 - 700 of 790 results