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Plasmid#127546PurposeA dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).DepositorInsertsUseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF, TEF2, URA3, bla (E. coli), and pTpA+MCSAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only
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pCMV-OptoSTIM1
Plasmid#70159PurposeMammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)DepositorInsertOptoSTIM1 (STIM1 Mustard Weed, Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag
Plasmid#216695PurposeSynchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)DepositorInsertsUseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-ATF4 mScarlet NLS
Plasmid#115970PurposeER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)DepositorInsertactivating transcription factor 4 (ATF4 Synthetic, Human)
UseRetroviralTagsHA tag, c-myc NLS, and mScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoSTIM1(Cry2(D387A))
Plasmid#70160PurposeLight-insensitive mutant of OptoSTIM1DepositorInsertOptoSTIM1(Cry2(D387A)) (STIM1 Mustard Weed, Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS-FCER1G-1
Plasmid#109192PurposeEncodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression systemDepositorInsertHuman full-length FCER1G, codon-optimized (FCER1G Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
NET23 pEGFP-N2 (1174)
Plasmid#62037Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET23 (STING1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-FLp300
Plasmid#61356Purposeencodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-Nm-dCas9-p300 Core
Plasmid#61365Purposeencodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertNm-dCas9-p300 Core (EP300 Synthetic, Human, Neisseria meningiditis)
UseCRISPR and Synthetic BiologyTagsHA tag and NLSExpressionMammalianMutationD16A, D587A, H588A, N611A in Nm Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core
Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAd-M3cherry
Plasmid#61041PurposeThis plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.DepositorUseAdenoviralTagsExpressionMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (Y1467F)
Plasmid#61362Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; Y1467F mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (1645/1646 RR/EE)
Plasmid#61359Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (1396/1397 SY/WW)
Plasmid#61363Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (C1204R)
Plasmid#61361Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; C1204R mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)
Plasmid#61364Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZH_tdPP7cp_mCherry
Plasmid#118267PurposeLow-noise, tetracycline inducible expression of tdPP7cp-mCherryDepositorInserttdPP7cp-mCherry
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH_mPP7cp_mCerulean3
Plasmid#118262PurposeLow-noise, tetracycline inducible expression of mPP7cp-mCerulean3DepositorInsertmPP7cp-mCerulean3
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH_tdPP7cp_mCerulean3
Plasmid#118263PurposeLow-noise, tetracycline inducible expression of tdPP7cp-mCerulean3DepositorInserttdPP7cp-mCerulean3
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH_mPP7cp_mCherry
Plasmid#118266PurposeLow-noise, tetracycline inducible expression of mPP7cp-mCherryDepositorInsertmPP7cp-mCherry
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH_tdPP7cp_ECFP
Plasmid#118265PurposeLow-noise, tetracycline inducible expression of tdPP7cp-ECFPDepositorInserttdPP7cp-ECFP
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH_mPP7cp_ECFP
Plasmid#118264PurposeLow-noise, tetracycline inducible expression of mPP7cp-ECFPDepositorInsertmPP7cp-ECFP
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBL7058
Plasmid#167227Purposeoverexpression of orthogonal T7 RNAPDepositorInsertJ23119-AKSIRV
UseSynthetic BiologyTags6X HisExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7055
Plasmid#167224Purposeorthogonal T7 RNAP sfGFP reporterDepositorInsertpRV-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7057
Plasmid#167226Purposeorthogonal T7 RNAP sfGFP reporterDepositorInsertpIRH-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7065
Plasmid#167234Purposecell-free cascaded nitrate sensorDepositorInsertpNar-AKSIRV
UseSynthetic BiologyTags6X HisExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7066
Plasmid#167235Purposecell-free cascaded mercury sensorDepositorInsertpMer-AKSIRV
UseSynthetic BiologyTags6X HisExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7069
Plasmid#167238Purposemalachite green aptamer tagged sfGFPDepositorInsertJ23119-sfGFP-MGA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7070
Plasmid#167239Purposemalachite green aptamer tagged sfGFPDepositorInsertpT7-sfGFP-MGA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7090
Plasmid#167252Purposecell-free mercury sensor with enzymatic outputDepositorInsertpMer-XylE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7091
Plasmid#167253Purposecell-free arsenic sensor with enzymatic outputDepositorInsertpArs-XylE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7093
Plasmid#167254Purposecell-free lead sensor with XylE outputDepositorInsertpPbr-XylE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7042
Plasmid#167213Purposeoverexpression of MerR in E. coli for cell-free mercury sensingDepositorInsertMerR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7047
Plasmid#167217Purposeoverexpression of CadR in E. coli for cell-free cadmium sensingDepositorInsertCadR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7049
Plasmid#167219Purposecell-free arsenic sensing reporterDepositorInsertpArs-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7050
Plasmid#167220Purposecell-free mercury sensing reporterDepositorInsertpMer-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7051
Plasmid#167221Purposecell-free cadmium sensing reporterDepositorInsertpCad-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7053
Plasmid#167222Purposecell-free lead sensing reporterDepositorInsertpPbr-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7054
Plasmid#167223Purposecell-free copper sensing reporterDepositorInsertpCue-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7064
Plasmid#167233Purposecell-free cascaded cadmium sensorDepositorInsertpCad-AKSIRV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT3TS
Plasmid#31830DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterLacAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJBL7068
Plasmid#167237PurposeHis-tagged sfGFP for protein purificationDepositorInsertpT7-6XHis-sfGFP-MGA
UseSynthetic BiologyTags6X HisExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7043
Plasmid#167214Purposeoverexpression of ArsR in E. coli for cell-free arsenic sensingDepositorInsertArsR EP3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7048
Plasmid#167218Purposecell-free nitrate sensing reporterDepositorInsertpNar-sfGFP
UseSynthetic BiologyTagsExpressionMutationydfJ115 promoterPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Snapin
Plasmid#118742PurposeExpresses EGFP tagged SnapinDepositorInsertSnapin cDNA (Snapin Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-U6
Plasmid#19799DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-Snapin
Plasmid#118743PurposeExpresses EGFP tagged SnapinDepositorInsertSnapin cDNA (Snapin Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only