Skip to main content
Addgene
Showing: 651 - 700 of 18313 results
  1. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid
    #216695
    Purpose
    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)
    Depositor
    Inserts
    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    Use
    Tags
    FIREtag, Streptavidin Binding Protein (SBP), and …
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pLHCX-ATF4 mScarlet NLS

    Plasmid
    #115970
    Purpose
    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)
    Depositor
    Insert
    activating transcription factor 4 (ATF4 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA tag, c-myc NLS, and mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid
    #61365
    Purpose
    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Insert
    Nm-dCas9-p300 Core (EP300 Synthetic, Human, Neisseria meningiditis)
    Use
    CRISPR and Synthetic Biology
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D16A, D587A, H588A, N611A in Nm Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid
    #61362
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  13. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  14. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. pZH_tdPP7cp_mCherry

    Plasmid
    #118267
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-mCherry
    Depositor
    Insert
    tdPP7cp-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. pZH_mPP7cp_mCerulean3

    Plasmid
    #118262
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-mCerulean3
    Depositor
    Insert
    mPP7cp-mCerulean3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pZH_tdPP7cp_mCerulean3

    Plasmid
    #118263
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-mCerulean3
    Depositor
    Insert
    tdPP7cp-mCerulean3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  18. pZH_mPP7cp_mCherry

    Plasmid
    #118266
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-mCherry
    Depositor
    Insert
    mPP7cp-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  19. pZH_tdPP7cp_ECFP

    Plasmid
    #118265
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-ECFP
    Depositor
    Insert
    tdPP7cp-ECFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. pZH_mPP7cp_ECFP

    Plasmid
    #118264
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-ECFP
    Depositor
    Insert
    mPP7cp-ECFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  21. pJBL7058

    Plasmid
    #167227
    Purpose
    overexpression of orthogonal T7 RNAP
    Depositor
    Insert
    J23119-AKSIRV
    Use
    Synthetic Biology
    Tags
    6X His
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  22. pJBL7055

    Plasmid
    #167224
    Purpose
    orthogonal T7 RNAP sfGFP reporter
    Depositor
    Insert
    pRV-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  23. pJBL7057

    Plasmid
    #167226
    Purpose
    orthogonal T7 RNAP sfGFP reporter
    Depositor
    Insert
    pIRH-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  24. pJBL7065

    Plasmid
    #167234
    Purpose
    cell-free cascaded nitrate sensor
    Depositor
    Insert
    pNar-AKSIRV
    Use
    Synthetic Biology
    Tags
    6X His
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  25. pJBL7066

    Plasmid
    #167235
    Purpose
    cell-free cascaded mercury sensor
    Depositor
    Insert
    pMer-AKSIRV
    Use
    Synthetic Biology
    Tags
    6X His
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  26. pJBL7069

    Plasmid
    #167238
    Purpose
    malachite green aptamer tagged sfGFP
    Depositor
    Insert
    J23119-sfGFP-MGA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  27. pJBL7070

    Plasmid
    #167239
    Purpose
    malachite green aptamer tagged sfGFP
    Depositor
    Insert
    pT7-sfGFP-MGA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  28. pJBL7090

    Plasmid
    #167252
    Purpose
    cell-free mercury sensor with enzymatic output
    Depositor
    Insert
    pMer-XylE
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  29. pJBL7091

    Plasmid
    #167253
    Purpose
    cell-free arsenic sensor with enzymatic output
    Depositor
    Insert
    pArs-XylE
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  30. pJBL7093

    Plasmid
    #167254
    Purpose
    cell-free lead sensor with XylE output
    Depositor
    Insert
    pPbr-XylE
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  31. pJBL7042

    Plasmid
    #167213
    Purpose
    overexpression of MerR in E. coli for cell-free mercury sensing
    Depositor
    Insert
    MerR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  32. pJBL7047

    Plasmid
    #167217
    Purpose
    overexpression of CadR in E. coli for cell-free cadmium sensing
    Depositor
    Insert
    CadR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  33. pJBL7049

    Plasmid
    #167219
    Purpose
    cell-free arsenic sensing reporter
    Depositor
    Insert
    pArs-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  34. pJBL7050

    Plasmid
    #167220
    Purpose
    cell-free mercury sensing reporter
    Depositor
    Insert
    pMer-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  35. pJBL7051

    Plasmid
    #167221
    Purpose
    cell-free cadmium sensing reporter
    Depositor
    Insert
    pCad-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  36. pJBL7053

    Plasmid
    #167222
    Purpose
    cell-free lead sensing reporter
    Depositor
    Insert
    pPbr-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  37. pJBL7054

    Plasmid
    #167223
    Purpose
    cell-free copper sensing reporter
    Depositor
    Insert
    pCue-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  38. pJBL7064

    Plasmid
    #167233
    Purpose
    cell-free cascaded cadmium sensor
    Depositor
    Insert
    pCad-AKSIRV
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  39. pJBL7068

    Plasmid
    #167237
    Purpose
    His-tagged sfGFP for protein purification
    Depositor
    Insert
    pT7-6XHis-sfGFP-MGA
    Use
    Synthetic Biology
    Tags
    6X His
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  40. pJBL7043

    Plasmid
    #167214
    Purpose
    overexpression of ArsR in E. coli for cell-free arsenic sensing
    Depositor
    Insert
    ArsR EP3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  41. pJBL7048

    Plasmid
    #167218
    Purpose
    cell-free nitrate sensing reporter
    Depositor
    Insert
    pNar-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ydfJ115 promoter
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  42. pEGFP-Snapin

    Plasmid
    #118742
    Purpose
    Expresses EGFP tagged Snapin
    Depositor
    Insert
    Snapin cDNA (Snapin Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  43. pCAG-EGFP-Snapin

    Plasmid
    #118743
    Purpose
    Expresses EGFP tagged Snapin
    Depositor
    Insert
    Snapin cDNA (Snapin Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 651 - 700 of 18313 results