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pGEX-Nedd4-C / 2523 JM15

Plasmid

#13311

Depositor

Allan Weissman

Publication

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 1-422 deleted

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEGFP-h53BP1 (siRNA resistant)

Plasmid

#110301

Purpose

Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

Depositor

Chris Kok-Lung Chan

Publication

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

GAACGAGGA to GAGCGGGGC

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pC003 - LshC2C2 locus into pACYC184 for spacer cloning

Plasmid

#79152

Purpose

LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Mutation

*

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

Plasmid

#86827

Purpose

Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

Depositor

Carl Novina

Publication

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

FLAG-NLS-MS2-eGFP

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pRK-flag-USP19

Plasmid

#78597

Purpose

Express flag-USP19 in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 (USP19 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Str-Ii_SBP-mCherry-Golgin84

Plasmid

#65304

Purpose

synchronize trafficking of Golgin-84 from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3_UnaG-Flag-Sec61B

Plasmid

#83413

Purpose

Expressed N-term tagged UnaG on Sec61B

Depositor

Hyun-Woo Rhee

Publication

Park et al ACS Chem Biol. 2016 May 27.

Insert

UnaG-Sec61B (SEC61B Human, Synthetic)

Expression

Mammalian

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CHMP2A_GFP_N_term

Plasmid

#31805

Depositor

Daniel Gerlich

Publication

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP2A (CHMP2A Human)

Expression

Mammalian

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGEX-Nedd4-N / 2524 JM16

Plasmid

#13312

Depositor

Allan Weissman

Publication

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa1-52 and aa422-777 deleted

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pLminP_Luc2P_RE4

Plasmid

#90338

Purpose

NRF2 - Antioxidant response element gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NRF2

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Carol Gross, Jason Peters, Stanley Qi

Publication

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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SM272_EF1A-dHyperLbCas12a-VPR-T2A-EGFP

Plasmid

#244210

Purpose

Mammalian expression of dHyperLbCas12a-VPR with EGFP selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEN-CAG-mRFP-PCNA pc2729

Plasmid

#166040

Purpose

Expresses mRFP tagged human PCNA in mammalian cells.

Depositor

Cristina Cardoso

Publication

Heinz et al Nucleic Acids Res. 2018 Jul 6;46(12):611

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBW759_pCAG-PV2-lox2272-GFP-BGHpA

Plasmid

#87575

Purpose

PV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full Adder

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-WBP2

Plasmid

#27478

Depositor

Marius Sudol

Publication

Chen et al J Biol Chem. 1997 Jul 4. 272(27):17070-7

Insert

WW domain binding protein-2 (WBP2 Human)

Tags

Flag

Expression

Mammalian

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
EGFP-E-Syt3

Plasmid

#66832

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Publication

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt3 (ESYT3 Human)

Tags

GFP

Expression

Mammalian

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-GFP1-10

Plasmid

#78591

Purpose

Express mCherry-GFP1-10 in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

GFP 1-10

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AgDD

Plasmid

#78289

Purpose

create protein aggregates in living cells

Depositor

Thomas Wandless

Publication

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Tags

MLALKLAGLDI

Expression

Mammalian

Promoter

pCAG

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3_erGAP3

Plasmid

#78118

Purpose

Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

Depositor

Teresa Alonso, Javier García-Sancho

Publication

Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

Insert

erGAP3

Tags

KDEL and calreticulin signal peptide

Expression

Mammalian

Mutation

S175G, D180Y, I167V and L15Q in GFP moiety and D…

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJC2722 - pCDNA4TO-CMV-3xFLAG-Halo-AARS1

Plasmid

#250254

Purpose

cDNA expression of affinity-tagged AARS1.

Depositor

Jacob Corn

Publication

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

AARS1 (AARS Human)

Tags

3xFLAG-Halo

Expression

Mammalian

Promoter

CMV

Available since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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