We narrowed to 396 results for: 42230

1. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pX330-sgRNA_Dgcr8_2

   Plasmid

   #73526

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

2. N-Terminal Halo XRCC4

   Plasmid

   #207541

   Purpose

   Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. N-terminal Halo-Ku70

   Plasmid

   #207547

   Purpose

   Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   HaloTag with flanked by human Ku70 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA TR KO 2

   Plasmid

   #207610

   Purpose

   sgRNA 2 to knockout TR by replacement with a PuroR cassette

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   TCAGGCCGCAGGAAGAGGAA
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA TR KO 1

   Plasmid

   #207609

   Purpose

   sgRNA 1 to knockout TR by replacement with a PuroR cassette

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   ACCCTAACTGAGAAGGGCGT
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. px330-mcherry

   Plasmid

   #98750

   Purpose

   Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

   Depositor

   Jinsong Li

   Article

   Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-EN479

   Plasmid

   #86234

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nickase and sgRNA against mouse rosa26 locus
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-EN1201

   Plasmid

   #92144

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
   

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330_SpCas9_USP7 Exon 3 gRNA

   Plasmid

   #131257

   Purpose

   pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   humanised S. pyogenes Cas9 nuclease
   

   Expression

   Mammalian

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX-evoCas9

    Plasmid

    #107550

    Purpose

    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

    Depositor

    Anna Cereseto

    Article

    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

    Insert

    Humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    M495V/Y515N/K526E/R661Q

    Promoter

    CBh

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKC123

    Plasmid

    #134240

    Purpose

    expresses gRNA targeting CENP A 3'

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP A 3' CRISPR construct
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-PCNT

    Plasmid

    #227284

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of PCNT (PCNT Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xMS2 TR knockin sgRNA 1

    Plasmid

    #207607

    Purpose

    sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    cgactcgcccggcagcgcac
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xMS2 TR knockin sgRNA 2

    Plasmid

    #207608

    Purpose

    sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    acccccaaacctgactgact
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKM328

    Plasmid

    #134243

    Purpose

    Mammalian expression construct expressing sgRNA against PLK1

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    gRNA targeting PLK1 3'
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-CENPA

    Plasmid

    #227282

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CENPA (CENPA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-RPA194

    Plasmid

    #247352

    Purpose

    Expresses SpCas9 and a sgRNA targeting the human RPA194 loci for knock-in.

    Depositor

    Kazuhiro Maeshima

    Article

    Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

    Insert

    RPA194 (POLR1A Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Nov. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-CETN2

    Plasmid

    #227291

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CETN2 (CETN2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

    Plasmid

    #85041

    Purpose

    This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    loxP-stop-loxP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-(BsaI)_CBh-Cas9

    Plasmid

    #135010

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Tags

    3xFLAG (N terminal on insert)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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