We narrowed to 143,240 results for: ins;

1. binder/tag-WT-FAK-insertionA

   Plasmid

   #190439

   Purpose

   The tag is SsrA (AANDENY). Tag is inserted after R35 of FAK.

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   FAK (Ptk2 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   SsrA inserted after aa 35

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1 Insulator ObLiGaRe/EPB71

   Plasmid

   #90018

   Purpose

   Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

   Depositor

   Eric-Paul Bennett

   Article

   Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

   Insert

   MCS flanked by insulator and inverted ZFN binding sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. R777-E108 Hs.INSRR-nostop

   Plasmid

   #70392

   Purpose

   Gateway ORF clone of human INSRR [NM_014215.2] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   INSRR (INSRR Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmTol-ins-UAS-R1R3-CC

   Plasmid

   #67745

   Purpose

   miniTol Destination vector with MAR-flanked UAS

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3796-38

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

   Plasmid

   #67687

   Purpose

   UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3796-38

   Insert

   dusp6 (dusp6 Zebrafish)
   

   Use

   Tags

   6xmyc-NLS-tdTomato-2A

   Expression

   Mutation

   S159A, S196A

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins

   Plasmid

   #31440

   Depositor

   Gerhart Ryffel

   Article

   Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Cre/Lox; Flp/frt

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A263insGG

   Promoter

   Available since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pStreptagIIx2 Alfatag 3C InsR EABR

   Plasmid

   #234996

   Purpose

   For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

   Depositor

   Daniel Leahy, Monica Gonzalez-Magaldi

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   InsR-EABR (INSR Human)
   

   Use

   Tags

   2xStreptag-II, Alfatag, HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF

   Plasmid

   #200865

   Purpose

   AjTn6022_TniQ expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   AjTn6022_TnsF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-601[insert(+1) TA-rich]

   Plasmid

   #221196

   Purpose

   601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   601[insert(+1)TA-rich]
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   601 has an additional mutation added 22 bp from d…

   Promoter

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX304-HsTtyh1-TwinStrep-His

    Plasmid

    #211332

    Purpose

    Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Tweety homology protein 1
    

    Use

    Lentiviral

    Tags

    TwinStrep, 10xHis

    Expression

    Mammalian

    Mutation

    W417R

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-HsProm1s1-TwinStrep-His

    Plasmid

    #210816

    Purpose

    Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1-His6-E3(1)-CUbo-TwinStrep

    Plasmid

    #212712

    Purpose

    Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Tags

    6xHis and CUbo-TwinStrep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDestTol2pA ins:Apollo-NADP+ mVenus

    Plasmid

    #210518

    Purpose

    Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

    Depositor

    Jonathan Rocheleau

    Article

    Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

    Insert

    Apollo-NADP+
    

    Use

    Zebrafish expression, tol2 kit gateway-compatible

    Tags

    mVenus

    Expression

    Mutation

    Promoter

    Zebrafish insulin promoter

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS2409-Tier1-PhCMV-FURINsite

    Plasmid

    #169488

    Purpose

    Tier-1 vector encoding PhCMV-controlled FURIN protease recognition site (PhCMV-FURINsite-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Furin cleavage site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCh-2xFKBP (no insert) (pBS1139)

    Plasmid

    #185322

    Purpose

    The empty vector used for the FKBP experiments in our paper

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

16. pG.Lantern-INS-SM50-GFP

    Plasmid

    #135594

    Purpose

    Propogation of Insulator-SM50 Promoter-GFP DNA for microinjection to visualize Insulator-GFP promoter activity in S. purpuratus

    Depositor

    Koji Akasaka, Mamiko Yajima

    Article

    Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

    Insert

    INS-SM50 promoter (SM50 )
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Green Lantern GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pR0306 CcaCas13b His6-TwinStrep-SUMO

    Plasmid

    #182687

    Purpose

    Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    CcaCas13b
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG001 His6-TwinStrep-SUMO-AapCas12b

    Plasmid

    #153162

    Purpose

    Bacterial expression plasmid for AapCas12b

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

    Insert

    AapCas12b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINS-USP21 (USP, aa 196-565)

    Plasmid

    #61585

    Purpose

    Expresses human USP21 (USP domain) in E. coli.

    Depositor

    David Komander

    Article

    Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

    Insert

    USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Deleted aa 1-195.

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid

    #183790

    Purpose

    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Inserts

    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    G>A in the gRNA scaffold- please see depositor…

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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