We narrowed to 148,387 results for: ins;

1. proinsulin-msGFP2 (C48S C70S)

   Plasmid

   #218956

   Purpose

   Expresses proinsulin fused to msGFP2 (C48S C70S) in bacteria

   Depositor

   Benjamin Glick

   Insert

   proinsulin-msGFP2 C48S C70S
   

   Tags

   proinsulin

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. INS-GFP-CD19-EPT

   Plasmid

   #210469

   Purpose

   Donor plasmid for knock-in GFP-CD19 into the human INS locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
   

   Use

   CRISPR

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMS17: pTwinStrep-SUMO-AvTnsD

   Plasmid

   #168150

   Purpose

   AvTnsD expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   AvCAST TnsD
   

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS15: pTwinStrep-SUMO-AvTnsB

   Plasmid

   #168148

   Purpose

   AvTnsB expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   AvCAST TnsB
   

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS16: pTwinStrep-SUMO-AvTnsC

   Plasmid

   #168149

   Purpose

   AvTnsC expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   AvCAST TnsC
   

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS14: pTwinStrep-SUMO-AvTnsA

   Plasmid

   #168147

   Purpose

   AvTnsA expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   AvCAST TnsA
   

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. binder/tag-WT-FAK-insertionB

   Plasmid

   #190440

   Purpose

   The tag is SsrA (AANDENY). Tag is inserted after D405 of FAK.

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   FAK (Ptk2 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   SsrA inserted after aa 405

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS32: pTwinStrep-SUMO-PmcTnsC

   Plasmid

   #168165

   Purpose

   PmcTnsC expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsC
   

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p240 pPr-CREM/Ins

   Plasmid

   #8405

   Purpose

   Androgen-inducible expression of Cre, with chimeric/β-globin intron within the Cre coding sequence and chicken globin insulators.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   probasin driven CREM with insulators
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   modified Cre

   Promoter

   Probasin promoter

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pLL3.7 shRNA against CRTC2

    Plasmid

    #72711

    Purpose

    Lentiviral expression of shRNA against CRTC2 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC2
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pAC8-TwinStrep-3C-DrHDAC6 (40-831)

    Plasmid

    #180226

    Purpose

    Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

    Depositor

    Patrick Matthias

    Article

    Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

    Insert

    Histone deacetylase 6 (hdac6 Zebrafish)
    

    Tags

    TwinStrep-3C

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMS33: pTwinStrep-SUMO-PmcTnsD

    Plasmid

    #168166

    Purpose

    PmcTnsD expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsD
    

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL3.7 shRNA against CRTC3

    Plasmid

    #72712

    Purpose

    Lentiviral expression of shRNA against CRTC3 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC3
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pLL3.7 shRNA against CRTC1

    Plasmid

    #72710

    Purpose

    Lentiviral expression of shRNA against CRTC1 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC1
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

15. binder/tag-WT-FAK-insertionA

    Plasmid

    #190439

    Purpose

    The tag is SsrA (AANDENY). Tag is inserted after R35 of FAK.

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    FAK (Ptk2 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    SsrA inserted after aa 35

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1 Insulator ObLiGaRe/EPB71

    Plasmid

    #90018

    Purpose

    Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

    Depositor

    Eric-Paul Bennett

    Article

    Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

    Insert

    MCS flanked by insulator and inverted ZFN binding sites
    

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. R777-E108 Hs.INSRR-nostop

    Plasmid

    #70392

    Purpose

    Gateway ORF clone of human INSRR [NM_014215.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    INSRR (INSRR Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmTol-ins-UAS-R1R3-CC

    Plasmid

    #67745

    Purpose

    miniTol Destination vector with MAR-flanked UAS

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Type

    Empty backbone

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

    Plasmid

    #67687

    Purpose

    UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    dusp6 (dusp6 Zebrafish)
    

    Tags

    6xmyc-NLS-tdTomato-2A

    Mutation

    S159A, S196A

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins

    Plasmid

    #31440

    Depositor

    Gerhart Ryffel

    Article

    Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Cre/Lox; Flp/frt

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A263insGG

    Available Since

    Oct. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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