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161,570 results

Showing: 2781 - 2800 of 161570 results

  1. 783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

    Plasmid
    #228362
    Purpose
    mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.
    Depositor
    Michael Boettcher
    Article
    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral; Rfxcas13d grna expression …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mU6 and hU6
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    228362-plasmid-map-sequence-id-457033

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  3. pET24a-VHH-std

    Plasmid
    #109417
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    109417-plasmid-map-sequence-id-218403

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  11. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190033
    Purpose
    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13X
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y672A,Y676A
    Promoter
    CBh, SV40, hU6
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190033-plasmid-map-sequence-id-371640

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  12. pcDNA3.1.Gαi3-LgB91

    Plasmid
    #134359
    Purpose
    Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi3-LgB91 (GNAI3 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134359-plasmid-map-sequence-id-306392

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  14. miniCRISPRoff-v1

    Plasmid
    #197030
    Purpose
    Vector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized miniCRISRPoff-v1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197030-plasmid-map-sequence-id-388972

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  16. pET24a-VHH-mCherry

    Plasmid
    #109421
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109421-plasmid-map-sequence-id-211587

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Showing: 2781 - 2800 of 161570 results