169,348 results

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mRFP-FKBP12

Plasmid

#67514

Purpose

FKBP12 only

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12 (FKBP1A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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HaloTag-beta2-adrenergic receptor

Plasmid

#66994

Purpose

Beta-2-adrenergic receptor tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-beta2-adrenergic receptor (ADRB2 Human)

Tags

Human Growth hormone Signal Sequence-HaloTag

Expression

Mammalian

Mutation

The signal sequence from human growth hormone was…

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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AiP13563 - pAAV-AiE0170h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3563)

Plasmid

#224029

Purpose

AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Puro

Plasmid

#125594

Purpose

Lentiviral expression of sgRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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dimericTomato-2xrGBD

Plasmid

#176098

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Lentiviral

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRIF-CFP

Plasmid

#13644

Depositor

Doug Golenbock

Insert

TRIF (TICAM1 Human)

Tags

CFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-ASAP5-WPRE

Plasmid

#225709

Purpose

Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5

Use

AAV

Promoter

human synapsin

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV INS2x mRFP-3MREs (AAV-beta)

Plasmid

#206928

Purpose

Beta cell-specific gene expression from AAV in human pancreatic islets

Depositor

Markus Grompe

Article

Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

Insert

mRFP

Use

AAV

Expression

Mammalian

Promoter

INSx2

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLIX403_Capture1_APOBEC_HA_P2A_mRuby

Plasmid

#183901

Purpose

Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

Capture1_APOBEC_HA_P2A_mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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PB-SAM

Plasmid

#102559

Purpose

Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Inserts

MS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Mutation

D10A and N863A in Cas9 and N55K in MS2

Promoter

CAG and EF1A

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Dendra2-Lifeact-7

Plasmid

#54694

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

Lifeact-Dendra2

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

Plasmid

#67977

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pYS29

Plasmid

#202086

Purpose

Parent vector for producing universal minicircle donors for HaloTag N-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

NT HygR-T2A-Halo Minicircle Parent

Use

CRISPR

Tags

HaloTag

Promoter

None

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMBP-MS2

Plasmid

#65104

Purpose

can be used to isolate RNA binding complexes

Depositor

Josep Vilardell

Article

Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

Insert

MS2

Tags

MBP

Expression

Bacterial

Mutation

double mutation (V75Q and A81G) that prevents oli…

Promoter

tac

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGluSnFR4s-NGR-WPRE

Plasmid

#234451

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al bioRxiv 2025.03.20.643984

Insert

iGluSnFR4s-NGR

Use

AAV

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pREP-NFAT2

Plasmid

#11788

Depositor

Anjana Rao

Insert

NFAT2 (NFATC1 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-EEA1

Plasmid

#174451

Purpose

Express EGFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with early endosome-targeting sequence

Tags

early endosomal antigen 1 (EEA1)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGY577

Plasmid

#166663

Purpose

Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

Cre: E340A, D341A

Promoter

CMV

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 YFP

Plasmid

#84910

Purpose

expression of YFP in mammalian cells

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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