161,488 results

1. 5E26 (PANK2)

   Plasmid

   #79516

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PANK2 (PANK2 Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis and TEV cleavage site

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   June 15, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. hCdh1 (1-125)

   Plasmid

   #8508

   Depositor

   Joan Ruderman

   Article

   Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

   Insert

   hCdh1 (1-125) (FZR1 Human)
   

   Use

   Retroviral

   Tags

   His

   Expression

   Mammalian

   Mutation

   Cdh1 N-terminus. Dominant Negative mutant.

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

3. SMC2 CRISPR

   Plasmid

   #140649

   Purpose

   SMC2 tagging CRISPR

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   SMC2 (SMC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTBRII / CEP-Zeo / Hygro

   Plasmid

   #16622

   Depositor

   Bert Vogelstein

   Article

   Zhou et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Insert

   TGF beta type II receptor (TGFBR2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

5. 2XMyc-LRRK2-Kinase

   Plasmid

   #25071

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   2XMyc

   Expression

   Mammalian

   Mutation

   Contains only the kinase domain aa 1863-2218

   Promoter

   Available since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-CAG-tTA

   Plasmid

   #193338

   Purpose

   AAV-mediated expression of tTA under the CAG promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   tTA2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EC59103

   Plasmid

   #191785

   Purpose

   LbCas12a expression in plants

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   AtUbi10::Cas12a+ 8 introns-T-E9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D156R

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-PTBP1CCtoSS

   Plasmid

   #89157

   Purpose

   Recombinant expression of PTBP1 C250S,C251S in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
   

   Use

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHAGE-PIK3CA-R88Q

   Plasmid

   #116538

   Purpose

   Lentiviral expression of PIK3CA R88Q

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   R88Q

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-4T-1_RAF1 (51-131)

    Plasmid

    #119218

    Purpose

    purification of GST-RAF1 RBD from E. coli

    Depositor

    Helen Morrison

    Article

    Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

    Insert

    RAF1 aa 51-131 (RAF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE-PIK3CA-C420R

    Plasmid

    #116457

    Purpose

    Lentiviral expression of PIK3CA C420R

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C420R

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pD649-HAsp-IGSF8-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156912

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    IGSF8 (IGSF8 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221 Spinophilin

    Plasmid

    #87123

    Purpose

    Gateway donor vector for Spinophilin

    Depositor

    Anthony Baucum

    Article

    Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

    Insert

    Spinophilin (PPP1R9B Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRAM18dSFA[MCS]

    Plasmid

    #84685

    Purpose

    Can be used for the transformation of rickettsia

    Depositor

    Ulrike Munderloh

    Article

    Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-6P1-hPXR-LBD

    Plasmid

    #67769

    Purpose

    Expression GST-hPXR-LBD in bacteria

    Depositor

    Sridhar Mani

    Article

    Li et al J Biol Chem. 2013 May 10;288(19):13655-6

    Insert

    human pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains amino acids 107 to 434 of human PXR

    Promoter

    Tac

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAVS1-tdTomato

    Plasmid

    #159275

    Purpose

    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11

    Insert

    tdTomato
    

    Use

    AAV; S1 knockin donor vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7-EGFP-C1-HsYTHDF3_AB

    Plasmid

    #148313

    Purpose

    Mammalian Expression of HsYTHDF3

    Depositor

    Elisa Izaurralde

    Insert

    HsYTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124493

    Purpose

    Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipYFP(TEV cleavage seq) T2A mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA GFP10-RHOA

    Plasmid

    #133068

    Purpose

    Expresses RHOA GTPase with tripartite split GFP10

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    ras homolog family member A (RHOA Human)
    

    Use

    Tags

    GFP10 (split-GFP tripartite)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCH68 (dAsCas12a-3xKRAB piggyBac)

    Plasmid

    #217334

    Purpose

    piggyBac expression of dAsCas12a-3xKRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    dAsCas12a
    

    Use

    CRISPR

    Tags

    nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    E993A

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge