We narrowed to 16,947 results for: She
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pLenti spCas9 T2A TagRFP-T P2A puro
Plasmid#122200PurposeThe plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsspCas9
TagRFP-T
UseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_MDA5∆2CARD
Plasmid#216800PurposeExpresses melanoma differentiation-associated protein 5 lacking its 2 CARD domainsDepositorInsertmelanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
UseTagsExpressionMammalianMutation∆1-287PromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXP418
Plasmid#26843DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pXP422
Plasmid#26845DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
hSyn-FAS-DO-ReaChR-mCitrine-WPRE
Plasmid#64035PurposeExpresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructsDepositorInserthSyn-FAS-DO-ReaChR-mCitrine-WPRE
UseAAVTagsReaChR tagged with mCitrineExpressionMammalianMutationPromoterhSynAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBJKS-mPar4
Plasmid#53232Purposemammalian expression of mouse Par4DepositorInsertPar4 (F2rl3 Mouse)
UseTagsFLAG and bovine prolactin signal sequence (MDSKGS…ExpressionMammalianMutationDeletion of aa1-17 (native signal peptide)PromoterAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS11D-blast
Plasmid#158476PurposeConstitutive lentiviral expression of human TMPRSS11D.DepositorInsertTMPRSS11D (TMPRSS11D Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mMDM2-IRES-GFP
Plasmid#66954Purposeretroviral construct for expression of mouse Mdm2 cloned into the EcoRI siteDepositorInsertMdm2 (Mdm2 Mouse)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationPromoterMSCVAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-4EBP1-T37A/T46A/Y54A/L59A
Plasmid#136421PurposeStable overexpression of elF4EBP1 T37A/T46A/Y54A/L59ADepositorInserteIF4EBP1 (EIF4EBP1 Human)
UseLentiviralTagsExpressionMammalianMutationT37A/T46A/Y54A/L59APromoterCMVAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti puro HA-LMNB1
Plasmid#227591PurposeLentiviral plasmid expressing human LMNB1 proteinDepositorInsertLMNB1 (LMNB1 Human)
UseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHA AKT2 T309A/S474A
Plasmid#60128Purposeexpresses a mutant AKT2DepositorInsertprotein kinase B (AKT2 Human)
UseRetroviralTagsHAExpressionMutationSerine 474 and Threonine 309 changed to alaninesPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXP420
Plasmid#26844DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
VRK1
Plasmid#39067PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertVRK1 (VRK1 Human)
UseTagsHis TevExpressionBacterialMutationSurface mutant for crystallisation K34A, K35A, E3…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
EPB41L3A-c000
Plasmid#175458PurposeProtein expression in bacterial cells. Entire CDS, M1-D1087.DepositorInsertEPB41L3 (EPB41L3 Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
4RCI
Plasmid#64653PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertYTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceApril 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pLVX-CMV600
Plasmid#110723PurposeLentiviral system for reduced ectopic expression.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-Nsp14-TEV-6His
Plasmid#154952PurposeExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsertNsp14-TEV-6His (ORF1ab SARS-CoV-2)
UseTagsTEV-6HisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLEX307-CTSS-puro
Plasmid#158469PurposeConstitutive lentiviral expression of human CTSS.DepositorInsertCTSS (CTSS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-mKate2
Plasmid#51092PurposeForebrain principal neuron expression of oChIEF kinetic variant E163A/T199C with physically uncoupled mKate2 red fluorophoreDepositorInsertoChIEF(E163A/T199C)
UseAAVTagsP2A-mKate2ExpressionMammalianMutationE163A/T199CPromoterCaMKIIaAvailable sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNY-351
Plasmid#231048PurposeUsed for staining GFPDepositorInsertanti GFP DARPin fused to goat Fc
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR
Plasmid#68347PurposeAAV plasmid expressing the FlpO recombinase. with empty gRNADepositorInsertFlPO
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV FST-FLAG
Plasmid#175167PurposeLentiviral expression of human FST-FLAGDepositorInsertFST (FST Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXP318
Plasmid#26837DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-77:TERF2-mEGFP
Plasmid#168798PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human TERF2.DepositorInsertTERF2 (TERF2 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceApril 14, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-MAP3K5 (R256C)
Plasmid#47105PurposeExpresses mutant (R256C) human MAP3K5 in mammalian cellsDepositorInsertMAP3K5 (MAP3K5 Human)
UseTagsFLAGExpressionMammalianMutationchanged arginine 256 to cysteinePromoterCMVAvailable sinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ GEMIN6 myc HIS
Plasmid#71659PurposeMammalian expression of GEMIN6DepositorInsertGEMIN6 (GEMIN6 Human)
UseTagsHIS and mycExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-FLAG-mMdm2-IRES-GFP
Plasmid#66955PurposeRetroviral vector to express a FLAG-tagged mouse Mdm2DepositorInsertMdm2 (Mdm2 Mouse)
UseRetroviralTagsFLAG and IRES-GFPExpressionMammalianMutationPromoterMSCVAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TMPRSS2
Plasmid#158441PurposeGateway Cloning compatible entry vector for the human TMPRSS2 gene.DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEU-Gm23
Plasmid#53738PurposeUsed for in vitro transcription/translation of soybean His-tagged arginyl-tRNA synthetase in the Cell Free Sciences systemDepositorInsertarginyl-tRNA synthetase
UseSynthetic BiologyTagsHis tagExpressionMutationPromoterSP6Available sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
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Mouse-Dysferlin-Transcript-Variant1
Plasmid#60214PurposeMouse dysferlin transcript variant-1 (accession #NM_021469) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.DepositorInsertDysferlin (Dysf Mouse)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
mT2A-sfGFP-linker-iCre-ERT2-FNF
Plasmid#182341PurposeRecombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNFDepositorInsertT2A-sfGFP-linker-iCre-ERT2-FNF
UseMouse Targeting; Flp / frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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CAPZA1-TwinStrepII
Plasmid#83193PurposeExpression of tagged protein in mammalian cellsDepositorInsertCAPZA1 (Capza1 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS
Plasmid#191527PurposeDonor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locusDepositorInserthGEM(1-110)_mNG_hSLBP(18-108)
UseCRISPR and Synthetic BiologyTagsmNeonGreen (mNG) + 3xFLAG_TwinStrepExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXP416
Plasmid#26842DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRK5M-TIMP3
Plasmid#31715DepositorInsertTIMP3 (TIMP3 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-CMV300
Plasmid#110720PurposeLentiviral system for reduced ectopic expression.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLIPf-NK1R Control Plasmid
Plasmid#101125PurposeExpression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cellsDepositorInsertNeurokinin-1 Receptor, Truncated (TACR1 Human)
UseTags10xHis, CLIP-tag (CLIPf), and FLAG EpitopeExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103 BAG3 WW domain
Plasmid#104266PurposeBacterial expression of WW domain from BAG3DepositorInsertBAG3 WW domain (BAG3 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IV-3xFLAG/HA/NBL10
Plasmid#110568PurposeCre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tagDepositorInsert3xFLAG/HA-tagged anti-GFP VHH
UseAAV, Cre/Lox, and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Galectin-C-18
Plasmid#62745PurposeLocalization: Membrane Receptor, C1 Cloning Vector, Excitation:587, Emission:610DepositorInsertGalectin (LGALS1 Human)
UseTagsmCherryExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCXLE_hePOU_shp53
Plasmid#206397PurposeIntegration-free (episomal) expression of human ePOU and shRNA against p53.DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-Flag-RELISH
Plasmid#63750PurposeExpresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoterDepositorInsertRelish (Rel Fly)
UseTagsFlagExpressionInsectMutationPromoterpMTAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIC144
Plasmid#63067PurposeRetroviral expression of EGFP-TEV-S-tag CENP-K for protein localization and affinity purification (LAP)DepositorInsertCENP-K, IMAGE clone 3511065 (CENPK Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only
