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pBK-YE1-BE3

Plasmid

#85174

Purpose

Expresses YE1-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YE1-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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mKO2-SLBP(18-126)

Plasmid

#83914

Purpose

Fluorescent probe for S/G2 cell cycle transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mKO2-SLBP(18-126) (SLBP Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9P35S-N

Plasmid

#66191

Purpose

expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

cauliflower mosaic virus 35S promoter (P35S)

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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CHMP2A_GFP_N_term

Plasmid

#31805

Depositor

Daniel Gerlich

Article

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP2A (CHMP2A Human)

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pBF3060

Plasmid

#26853

Purpose

Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Insert

CreA recombinase

Use

Cre/Lox

Expression

Yeast

Mutation

N/A

Promoter

GAL1

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZAP70

Plasmid

#23887

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ZAP70 (ZAP70 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PTK2

Plasmid

#20610

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PTK2 (PTK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pBTR1

Plasmid

#22468

Depositor

Gary Pielak

Article

Olteanu et al Biochem Biophys Res Commun. 2003 Dec 19.

Insert

cytochrome c (CYCS Human)

Expression

Bacterial

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pmRFP_LAP2beta_IRES_puro2b

Plasmid

#21047

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

LAP2_beta (TMPO Rat)

Tags

mRFP

Expression

Bacterial and Mammalian

Available Since

July 14, 2009

Availability

Academic Institutions and Nonprofits only

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pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85008

Purpose

For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB454

Plasmid

#74836

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-Dsup

Plasmid

#90020

Purpose

To express GFP-tagged Dsup in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Golgi-Ras-LOCKR-S

Plasmid

#216499

Purpose

FRET-based reporter for Ras activity, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLN381 (FuGW-S(NFY)p-mKate2)

Plasmid

#105179

Purpose

synthetic promoter NFY drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(NFY)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLN592 (FuGW-S(USF1)p-mKate2)

Plasmid

#105204

Purpose

synthetic promoter USF1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(USF1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLN593 (FuGW-S(MAFK)p-mKate2)

Plasmid

#105205

Purpose

synthetic promoter MAFK drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(MAFK)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiFUCCI(CA)5

Plasmid

#223176

Purpose

Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

Depositor

Neville Sanjana

Article

Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

Insert

FUCCI(CA)5

Use

Lentiviral

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pER-mScarlet3_N1

Plasmid

#189772

Purpose

Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

ER-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide-zeo

Plasmid

#160091

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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