59,474 results

1. pLVX-opto-hCaspase-4 dCARD

   Plasmid

   #208785

   Purpose

   Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Cry2 mCherry Caspase4 dCARD (CASP4 Human)
   

   Use

   Lentiviral

   Tags

   Cry2 mCherry

   Expression

   Mammalian

   Mutation

   CARD domain is removed

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-C1-Nir1-LNS2

   Plasmid

   #220084

   Purpose

   high affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Tags

   mCherry-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGfa2-nLac

   Plasmid

   #53126

   Purpose

   Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.

   Depositor

   Michael Brenner

   Article

   Brenner et al J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.

   Insert

   Gfa2 (GFAP Human)
   

   Use

   Mouse Targeting

   Tags

   LacZ, NLS, and mP1

   Expression

   Mammalian

   Mutation

   contains -2163 to +47 (the human gfa2 segment), w…

   Promoter

   human GFAP

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. nLuc-GLP-1R-His-FLAG

   Plasmid

   #124831

   Purpose

   Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells

   Depositor

   Sam Gellman

   Article

   Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. d

   Insert

   GLP1R (GLP1R Human, Synthetic)
   

   Use

   Luciferase

   Tags

   FLAG, His6, and NanoLuc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPR v2-sgSLC7A11/xCT-1

   Plasmid

   #161818

   Purpose

   knock out SLC7A11/xCT in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

   Insert

   SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1-pGC-A

   Plasmid

   #186626

   Purpose

   pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

   Depositor

   Debra Hansen

   Article

   Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

   Insert

   NPR1 (NPR1 Human)
   

   Tags

   hemagglutinin (HA) signal peptide + His10-tag + T…

   Expression

   Insect

   Mutation

   deleted amino acids 1-32

   Promoter

   polyhedring

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Fab_8D3_2_H-H6

   Plasmid

   #176075

   Purpose

   Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   His6 tagged heavy chain of Fab_8D3_2
   

   Tags

   His tag

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Fab_8D3_2_L

   Plasmid

   #176076

   Purpose

   Express light chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   Light chain of Fab_8D3_2
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-hLC3B-pcDNA3.1

   Plasmid

   #40827

   Depositor

   David Rubinsztein

   Article

   Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

   Insert

   LC3B (MAP1LC3B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid

    #129593

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPR v2-sgLKB1 clone1

    Plasmid

    #162125

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 HA Arf6 ActQ67L

    Plasmid

    #10835

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 Q67L (ARF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q67L. Defective in GTP hydrolysis. Constitutive…

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1a-GFP-SFPQ-IRES-mCherry

    Plasmid

    #166950

    Purpose

    Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    GFP and mCherry

    Promoter

    eF1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. scFv-sfGFP-DNMT3A1

    Plasmid

    #102278

    Purpose

    The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

    Depositor

    Margaret Goodell

    Article

    Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

    Insert

    DNMT3A1 (DNMT3A Human)
    

    Use

    Lentiviral

    Tags

    scFV-sfGFP

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Neo-Bam p53 R175H

    Plasmid

    #16436

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R175H (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    CMV

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_OTX2_P2A_Hygro_Barcode

    Plasmid

    #120471

    Purpose

    Barcoded lentiviral vector to express OTX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    OTX2 (OTX2 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMIG-hNFATc2

    Plasmid

    #74050

    Purpose

    retroviral expression plasmid for human NFATc2/C

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc2, isoform C (NFATC2 Human)
    

    Use

    Retroviral

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    silent mutation A1723C in the sequence of NM_1730…

    Promoter

    pMSCV-LTRs

    Available Since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCWXPGR-pTF-betaCatenin

    Plasmid

    #114281

    Purpose

    TET-inducible expression of a constitutively active form of the BetaCatenin protein

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    Catenin beta 1 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    deletion of AA 1 to 45

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TLCV2-RB1

    Plasmid

    #87836

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Insert

    sgRB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Expression

    Mammalian

    Promoter

    Tight TRE promoter and U6

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-XRCC1

    Plasmid

    #215142

    Purpose

    Expresses mCherry-XRCC1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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