We narrowed to 13,038 results for: Mpl

1. pEBTet-SNAP-KIAA0841

   Plasmid

   #136810

   Purpose

   Mammalian expression of the centrosomal protein KIAA0841 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-KIAA0841 (HAUS5 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEBTet-SNAP-Centrin2

   Plasmid

   #136784

   Purpose

   Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Centrin2 (CETN2 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmiRGLO-ATF5-201

   Plasmid

   #117340

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR ATF5-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-FRT-TO-FH-Nsp15

   Plasmid

   #157721

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp15 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDEST27-GST-ATXN1 FL(2Q)

   Plasmid

   #21752

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains 2Q (2 glutamines) and there isno …

   Promoter

   CMV

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pAPM-D4 miR30-SETDB1 ts3

   Plasmid

   #115861

   Purpose

   SETDB1 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SETDB1 shRNA (SETDB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAPM-D4 miR30-SETDB1 ts2

   Plasmid

   #115860

   Purpose

   SETDB1 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SETDB1 shRNA (SETDB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CD8a-f-CTS-EGFP

   Plasmid

   #86063

   Purpose

   A mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains CD8a luminal and transmembrane domains a…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HOXB1 (human) HIS-tag pET

   Plasmid

   #8520

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB1 (HOXB1 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   PCR from cDNA clone

   Promoter

   Available since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pBABE hygro MEN1 L22R

    Plasmid

    #11021

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 L22R (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L22R

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3-HA/Bmal L95E

    Plasmid

    #47344

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L95E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3 HRPT2 136X

    Plasmid

    #11049

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 136X (CDC73 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

13. p2Flag CMV2-ZO-2-N

    Plasmid

    #27418

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Deletion of the carboxy-terminus of ZO-2, so only…

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5-FRT-TO-FH-Nsp7

    Plasmid

    #157689

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. HBM-mVEGF-C-HisTag-pENTR

    Plasmid

    #64066

    Purpose

    Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

    Insert

    vascular endothelial growth factor C (Vegfc Mouse)
    

    Use

    Tags

    His-tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAPM-D4 miR30-HDAC1 ts3

    Plasmid

    #115849

    Purpose

    HDAC1 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HDAC1 shRNA (HDAC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAPM-D4 miR30-HDAC1 ts2

    Plasmid

    #115848

    Purpose

    HDAC1 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HDAC1 shRNA (HDAC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJZC77

    Plasmid

    #62338

    Purpose

    sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    COM-KRAB
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT-TO-Nsp15-HF

    Plasmid

    #157722

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp15-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pJZC101

    Plasmid

    #62335

    Purpose

    sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    COM-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EZH2-TALEN_Right_No_FLAG

    Plasmid

    #79900

    Purpose

    Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Insert

    EZH2-TALEN_Right_No_FLAG (EZH2 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV-U6-UL29-egfp-U3-UL8

    Plasmid

    #166694

    Purpose

    To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

    Depositor

    Yujia Cai

    Article

    Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

    Insert

    UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pU6-(BsaI)_CBh-Cas9

    Plasmid

    #135010

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    3xFLAG (N terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBABE hygro MEN1 T344R

    Plasmid

    #11019

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 T344R (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    T344R

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

25. pJZC73

    Plasmid

    #62341

    Purpose

    sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    COM-KRAB
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HOXB3 (human) HIS-tag pET

    Plasmid

    #8524

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA 3.3 HA-TSPAN12^11-LEL

    Plasmid

    #115785

    Purpose

    Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    large extracellular loop of TSPAN12 replaced with…

    Promoter

    CMV

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET14b-mEKLF

    Plasmid

    #67834

    Purpose

    Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution

    Depositor

    James Bieker

    Article

    Armstrong et al Cell. 1998 Oct 2;95(1):93-104.

    Insert

    EKLF (Klf1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Full-length mEKLF is aa 20-376; amino acid 19 is …

    Promoter

    T7

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

29. [pSK497] pLJC5 Flag-Depdc5(AB)

    Plasmid

    #109341

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant AB

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5FRT/TO PP1gamma-2*Strep

    Plasmid

    #113506

    Purpose

    Expresses dual Strep-tagged PP1gamma in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    PP1gamma (PPP1CC Human)
    

    Use

    Tags

    Strep-Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Gal4-entry

    Plasmid

    #128014

    Purpose

    Vector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    Gal4-DBD-3xFlag
    

    Use

    Gal4 entry vector

    Tags

    Gal4-DBD-3xFlag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBABE hygro MEN1 W436R

    Plasmid

    #11018

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 W436R (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    W436R

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA3 HRPT2 227X

    Plasmid

    #11050

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 227X (CDC73 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

34. HA-VHL 1-187-pRc/CMV

    Plasmid

    #22079

    Depositor

    William Kaelin

    Article

    Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deletion mutant covering only aa 1-187 of wt VHL …

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Brm BDmut-Flag pShuttle

    Plasmid

    #122108

    Purpose

    Expression of full length Brm containing point mutations in its bromodomain in mammalian cells

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brm (SMARCA2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBABE hygro MEN1 P12L

    Plasmid

    #11020

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 P12L (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    P12L

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBABE hygro MEN1 A309P

    Plasmid

    #11023

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 A309P (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    A309P

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

    Plasmid

    #90011

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C S216A
    NBirA*-14-3-3e
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Ser 216 to Ala

    Promoter

    CMV

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV10/3xflag-Clock L57E/L113E/W284A

    Plasmid

    #47343

    Purpose

    triple mutation used for co-IP

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L57E/L113E/W284A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

40. p28BIOHTEV-LIC: nsp16_SARS-CoV-2

    Plasmid

    #167846

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp16

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp16
    

    Use

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pmiRGLO-LFNG-203

    Plasmid

    #117351

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR LFNG-203
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pX330-Utf1 gRNA

    Plasmid

    #128842

    Purpose

    gRNA for targeting mouse Utf1 locus using CRISPR-cas technique

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Utf1 gRNA (Utf1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. R725-X09-635: His6-tev-Hs.SPRED1(13-125)

    Plasmid

    #159581

    Purpose

    Baculovirus protein expression of His6-tev-Hs.SPRED1(13-125)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-tev-Hs.SPRED1(13-125) (SPRED1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBScMXT + mSlo1 MBr5/23

    Plasmid

    #16151

    Depositor

    Larry Salkoff

    Article

    Butler et al Science. 1993 Jul 9. 261(5118):221-4.

    Insert

    Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

45. pcDNA5-FRT-TO-Nsp2

    Plasmid

    #157682

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp2 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pCDNA3-HA/Bmal I317D

    Plasmid

    #47347

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    I317D

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

47. pEBTetBl-CLIP-Cep57

    Plasmid

    #136866

    Purpose

    Mammalian expression of the centrosomal protein Cep57 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep57 (CEP57 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA5-FRT-TO-Nsp2-HF

    Plasmid

    #157684

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp2-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. G protein alpha-o-R179C-EE

    Plasmid

    #55780

    Purpose

    This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    G protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
    

    Use

    Tags

    EE epitope was introduced internally with D167E a…

    Expression

    Bacterial and Mammalian

    Mutation

    Arginine 179 was replaced with cysteine

    Promoter

    CMV

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pmiRGLO-CD164-201

    Plasmid

    #117329

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR CD164-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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